预约演示

更新于：2025-05-07

气溶素

更新于：2025-05-07

基本信息

别名-

简介-

关联

项与气溶素相关的药物

Lung-H1

靶点

气溶素

作用机制

气溶素刺激剂

在研机构-

原研机构

University Of Victoria Innovation And Development Corp

在研适应症-

非在研适应症

非小细胞肺癌

最高研发阶段无进展

首次获批国家/地区-

首次获批日期1800-01-20

100 项与气溶素相关的临床结果

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100 项与气溶素相关的转化医学

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0 项与气溶素相关的专利（医药）

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327

项与气溶素相关的文献（医药）

2025-07-01·Fish & Shellfish Immunology

Dual-seq transcriptomics of Aeromonas salmonicida infection in Atlantic salmon (Salmo salar) primary macrophages reveals lysosome and apoptosis impairments

Article

作者: Soto-Davila, Manuel ; Gnanagobal, Hajarooba ; Vasquez, Ignacio ; Santander, Javier ; Hall, Jennifer R ; Hossain, Ahmed

A. salmonicida subsp. salmonicida is one of the oldest-known marine pathogens causing furunculosis in freshwater to marine fish species. A. salmonicida causes septicemia and fish death due to a systemic shock. Early stages of A. salmonicida infection, including intracellular macrophage infection, are not fully comprehended. Here, we conducted a dual RNA-seq study and functional analyses in Atlantic salmon primary macrophages infected with A. salmonicida to identify relevant genes for fish cellular immunity and A. salmonicida pathogenesis. At 1-h post-infection (hpi), A. salmonicida modulated the expression of genes associated with inflammation, fatty acids synthesis, and apoptosis. While at 2 hpi A. salmonicida hijacked pathways related to myeloid cell differentiation, cytoskeleton and actin filament organization, lysosome maturation, and apoptosis. In contrast, A. salmonicida upregulated genes encoding for hemolysin, aerolysin, type IVa pili, and T3SS effectors. In conclusion, these results suggest that A. salmonicida induces endocytosis, impairs lysosome maturation, and reduces apoptosis.

2025-03-01·Biotechnology and Bioengineering

High‐Efficiency Genome Editing in Naturally Isolated Aeromonas hydrophila and Edwardsiella Piscicida Using the CRISPR‐Cas9 System

Article

作者: Zhang, Dunhua ; Ma, Yuechao ; Feng, Jun ; Wang, Yi

ABSTRACTAeromonas hydrophila and Edwardsiella piscicida are significant bacterial pathogens in aquaculture, causing severe diseases and tremendous economic losses worldwide. Additionally, both of them can act as opportunistic pathogens in humans, leading to severe infections. Efficient genome editing tools for these pathogens are essential for understanding their pathogenic mechanisms and physiological behaviors, enabling the development of targeted strategies to control and mitigate their effects. In this study, we adapted the CRISPR‐Cas9 system for high‐efficiency, marker‐less genome editing in multiple naturally isolated strains of these two aquaculture pathogens. We developed a streamlined procedure that successfully generated deletion mutants of the aerA gene (encoding for aerolysin, a pore‐forming toxin that plays a critical role in the pathogenicity) and the gfp insertion mutants in three naturally isolated A. hydrophila strains. Additionally, we deleted five putative hemolysin‐encoding genes in both A. hydrophila ML10‐51K and its ∆aerA derivative. The same system was also applied to the naturally isolated E. piscicida S11‐285 strain, successfully deleting the ssaV gene (a component of the Type III Secretion System—a critical virulence mechanism in many pathogenic bacteria). The methodologies developed herein could be broadly applied to other pathogenic strains from natural environments, providing valuable tools for studying bacterial pathogenesis and aiding in the development of effective control strategies.

2025-03-01·Fish & Shellfish Immunology

Pathogenic Aeromonas veronii with the cheZ-mshK-aerA triple-gene mutant is attenuated and exhibits a potential candidate as a live attenuated vaccine

Article

作者: Pan, Xiaoyi ; Yu, Yongyao ; Lu, Jiahui ; Wei, Jinming ; Xu, Hao ; Liu, Kemei ; Li, Detao ; Li, Ziyang ; Ye, Hua ; Wu, Ronghua ; Li, Yun

Aeromonas veronii is a zoonotic pathogen that is commonly found in various aquatic environments and causes serious damage to the aquaculture industry. Anti-virulence strategies based on mutating the virulence factors are important antibiotic alternative methods against A. veronii infection. The type Ⅳ pili, polar flagella, and aerolysin are considered to be the major virulence factors. In this study, we constructed a triple-gene deletion mutant strain (cheZ/mshK/aerA, ΔCMA-AV) and evaluated its potential as a live attenuated vaccine (LAV) candidate. Pathogenicity test showed that the LD₅₀ of ΔCMA-AV in crucian carp (Carassius auratus) was 5.34 times higher than that of WT-AV. Further research found that the decline of pathogenicity was associated with the decrease of hemolysis, biofilm formation ability and expression levels of virulence-related genes (type IV pili and flagella). Our preliminary results on ΔCMA-AV as a LAV showed that crucian carp immunized with ΔCMA-AV at a concentration of 3 × 10⁶ CFU/mL did not show any evident pathological alterations or clinical symptoms. Non-specific immune indicators, including serum immune-related enzyme activities and the expression of immune-related genes in immune organs, showed an up-regulation trend in the ΔCMA-AV group. A. veronii specific antibody levels increased significantly from 2 to 4 weeks. The relative percent survival in ΔCMA-AV groups was 73.97 % ± 3.57 %, significantly higher than that of the inactivated A. veronii groups (53.73 % ± 10.87 %). These results indicate that the three genes (cheZ/mshK/aerA) play an important role in the pathogenicity of A. veronii, in additionΔCMA-AV could induce an effective immune response and provide strong protection against A. veronii in crucian carp without adverse effects.

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