更新于：2024-11-01

Glycosidases x β-N-乙酰氨基己糖苷酶

更新于：2024-11-01

基本信息

关联

项与 Glycosidases x β-N-乙酰氨基己糖苷酶相关的药物

OPT-33

靶点

β-N-乙酰氨基己糖苷酶

作用机制

β-N-乙酰氨基己糖苷酶抑制剂 [+1]

在研机构-

原研机构

Optimer Pharmaceuticals LLC

在研适应症-

非在研适应症

骨关节炎

最高研发阶段无进展

首次获批国家/地区-

首次获批日期1800-01-20

100 项与 Glycosidases x β-N-乙酰氨基己糖苷酶相关的临床结果

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100 项与 Glycosidases x β-N-乙酰氨基己糖苷酶相关的转化医学

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0 项与 Glycosidases x β-N-乙酰氨基己糖苷酶相关的专利（医药）

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项与 Glycosidases x β-N-乙酰氨基己糖苷酶相关的文献（医药）

2021-05-01·Plant Biology2区 · 生物学

Effects of heat treatment on metabolism of tobacco plants infected with Potato virus Y

2区 · 生物学

Article

作者: Hýsková, V. ; Doričová, V. ; Čeřovská, N. ; Synková, H. ; Henke, S. ; Gillarová, S. ; Bělonožníková, K. ; Ryšlavá, H. ; Kavan, D.

Abstract

Many factors affect successful virus propagation and plant defence responses. Heat shock protein (Hsp) expression after heat shock plays an ambiguous role in viral infection. On the one hand, Hsp70 participates in plant defence response; on the other hand, Hsp70 could interact with viral proteins and facilitate virus propagation.

Here, we studied metabolic adaptations of Nicotiana tabacum L. subjected to heat shock (42 °C, 2 h) before or after inoculating the plants with Potato virus Y (potyvirus).RT‐qPCR and ELISA were used for potyvirus quantification. Hsp70 and Hsp90 isoforms were analysed by Western blotting. Salicylic, quinic and chlorogenic acid content was determined by LC‐MS. The activity of Hatch–Slack enzymes (as markers of potyviral infection in tobacco) and glycosidases was assayed.

Application of heat shock before or after inoculation showed accelerated potyviral propagation in comparison with only inoculated plants. Plants exposed to heat shock and concurrently inoculated showed higher potyviral content, higher amount of Hsp70, together with late decline of quinic acid content and low chlorogenic acid content. Spread of potyviral infection correlated with enhanced salicylic acid content and activities of enzymes of the Hatch–Slack cycle, α‐ and β‐galactosidase, α‐mannosidase, α‐glucosidase and β‐N‐acetylhexosaminidase.

Heat shock proteins accelerate potyviral propagation. The lower weight cytosolic and mitochondrial Hsp70 (~50‐75 kDa) persist throughout the viral infection. Also, the plant defense response results in increase of salicylic and chlorogenic acids but decrease of quinic acid content.

2014-01-01·Organic & Biomolecular Chemistry3区 · 化学

Synthesis of the enantiomers of XYLNAc and LYXNAc: comparison of β-N-acetylhexosaminidase inhibition by the 8 stereoisomers of 2-N-acetylamino-1,2,4-trideoxy-1,4-iminopentitols

3区 · 化学

Article

作者: Shinpei Nakagawa ; R. Fernando Martínez ; Terry D. Butters ; Andreas F. G. Glawar ; George W. J. Fleet ; Atsushi Kato ; Isao Adachi ; Elizabeth V. Crabtree

The enantiomers of XYLNAc (2-N-acetylamino-1,2,4-trideoxy-1,4-iminoxylitol) are prepared from the enantiomers of glucuronolactone; the synthesis of the enantiomers of LYXNAc (2-N-acetylamino-1,2,4-trideoxy-1,4-iminolyxitol) from an L-arabinono-δ-lactone and a D-ribono-δ-lactone is reported. A comparison is made of the inhibition of β-N-acetylhexosaminidases (HexNAcases) and α-N-acetylgalactosaminidase (α-GalNAcase) by 8 stereoisomeric 2-N-acetylamino-1,2,4-trideoxy-1,4-iminopentitols; their N-benzyl derivatives are better inhibitors than the parent compounds. Both XYLNAc and LABNAc are potent inhibitors against HexNAcases. None of the compounds show any inhibition of α-GalNAcase.

2014-01-01·Journal of Proteomics & Bioinformatics

Mass Spectrometric Analysis of Whole Secretome and Amylase-precipitated Secretome Proteins from Streptococcus gordonii

Article

作者: Haase, Em ; Maddi, A ; Scannapieco, Fa

Oral biofilm (dental plaque) is formed by the initial adhesion of "pioneer species" to salivary proteins that form the dental pellicle on the tooth surface. One such pioneer species, Streptococcus gordonii, is known to bind salivary amylase through specific amylase-binding proteins such as amylase-binding protein A (AbpA). Recent studies have demonstrated that once bound, salivary amylase appears to modulate gene expression in S. gordonii. However, it is not known if this amylase-induced gene expression leads to secretion of proteins that play a role in plaque biofilm formation. In this study we examined the differences in secreted proteomes between S. gordonii KS1 (wild type) and AbpA-deficient (ΔAbpA) strains. We also examined the differentially precipitated secretome proteins following incubation with salivary amylase. The culture supernatants from KS1 and ΔAbpA were analyzed by nano-LC/MS/MS to characterize the whole secreted proteomes of the KS1 and ΔAbpA. A total of 107 proteins were identified in the KS1 and ΔAbpA secretomes of which 72 proteins were predicted to have an N-terminal signal peptide for secretion. Five proteins were differentially expressed between the KS1 and ΔAbpA secretomes; AbpA and sortase B were expressed exclusively by KS1, whereas Gdh, AdcA and GroEL were expressed only by ΔAbpA. Incubation of culture supernatants from KS1 and ΔAbpA with amylase (50 μg/ml) at room temperature for 2 h resulted in the differential precipitation of secretome proteins. Hypothetical protein (SGO_0483), cation-transporting ATPase YfgQ (Aha1), isocitrate dehydrogenase (Icd), sortase A (SrtA), beta-N-acetylhexosaminidase (SGO_0405), peptide chain release factor 1(PrfA) and cardiolipin synthase (SGO_2037) were precipitated by amylase from the KS1 culture supernatant. Among the identified secreted proteins and amylase-precipitated proteins, transcriptional regulator LytR (SGO_0535) and cation-transporting ATPase YfgQ (Aha1) are potential signaling proteins.