Article
作者: Kishi, Hiroyuki ; Matsushita, Hirokazu ; Adachi, Katsutoshi ; Takahashi, Yusuke ; Doi, Kiyoshi ; Stratford, Richard ; Masago, Katsuhiro ; Fukuyama, Takashi ; Clancy, Trevor ; Miura, Daiki ; Demachi-Okamura, Ayako ; Shinohara, Shuichi ; Muraoka, Daisuke ; Hamana, Hiroshi ; Yamashita, Yoshiko ; Kuroda, Hiroaki ; Matsui, Takuya ; Watanabe, Fumiaki ; Nishida, Reina ; Sugita, Yusuke ; Fukushima, Yasunori ; Onoue, Kousuke ; Tanaka, Yuki ; Komuro, Hiroyasu ; Takashima, Chieko ; Onoguchi, Kazuhide ; Yamaguchi, Rui ; Iwata, Hisashi ; Ohki, Takashi ; Shigematsu, Yoshiki
BackgroundCD8+tumor infiltrating lymphocytes (TILs) are often observed in non-small cell lung cancers (NSCLC). However, the characteristics of CD8+TILs, especially T-cell populations specific for tumor antigens, remain poorly understood.MethodsHigh throughput single-cell RNA sequencing and single-cell T-cell receptor (TCR) sequencing were performed on CD8+TILs from three surgically-resected lung cancer specimens. Dimensional reduction for clustering was performed using Uniform Manifold Approximation and Projection. CD8+TIL TCR specific for the cancer/testis antigen KK-LC-1 and for predicted neoantigens were investigated. Differentially-expressed gene analysis, Gene Set Enrichment Analysis (GSEA) and single sample GSEA was performed to characterize antigen-specific T cells.ResultsA total of 6998 CD8+T cells was analyzed, divided into 10 clusters according to their gene expression profile. An exhausted T-cell (exhausted T (Tex)) cluster characterized by the expression ofENTPD1(CD39),TOX,PDCD1(PD1),HAVCR2(TIM3) and other genes, and by T-cell oligoclonality, was identified. The Tex TCR repertoire (Tex-TCRs) contained nine different TCR clonotypes recognizing five tumor antigens including a KK-LC-1 antigen and four neoantigens. By re-clustering the tumor antigen-specific T cells (n=140), it could be seen that the individual T-cell clonotypes were present on cells at different stages of differentiation and functional states even within the same Tex cluster. Stimulating these T cells with predicted cognate peptide indicated that TCR signal strength and subsequent T-cell proliferation and cytokine production was variable but always higher for neoantigens than KK-LC-1.ConclusionsOur approach focusing on T cells with an exhausted phenotype among CD8+TILs may facilitate the identification of tumor antigens and clarify the nature of the antigen-specific T cells to specify the promising immunotherapeutic targets in patients with NSCLC.