更新于：2024-11-01

elastin x albumin

更新于：2024-11-01

基本信息

关联

项与 elastin x albumin 相关的药物

flexibzumab

靶点

albumin x elastin

作用机制

albumin调节剂 [+1]

在研机构

Clemson University

原研机构

Clemson University

在研适应症-

非在研适应症-

最高研发阶段临床前

首次获批国家/地区-

首次获批日期1800-01-20

100 项与 elastin x albumin 相关的临床结果

登录后查看更多信息

100 项与 elastin x albumin 相关的转化医学

登录后查看更多信息

0 项与 elastin x albumin 相关的专利（医药）

登录后查看更多信息

项与 elastin x albumin 相关的文献（医药）

2020-01-01·The American Journal of Pathology2区 · 医学

Single-Cell RNA Sequencing Identifies Yes-Associated Protein 1–Dependent Hepatic Mesothelial Progenitors in Fibrolamellar Carcinoma

2区 · 医学

Article

作者: Hyun, Jeongeun ; Gibson, Jason R ; Du, Kuo ; Oh, Seh-Hoon ; Guy, Cynthia D ; Diehl, Anna Mae E ; Ribar, Thomas ; Gregory, Simon G ; Premont, Richard T ; Hsu, David S ; Jewell, Mark L

Fibrolamellar carcinoma (FLC) is characterized by in-frame fusion of DnaJ heat shock protein family (Hsp40) member B1 (DNAJB1) with protein kinase cAMP-activated catalytic subunit α (PRKACA) and by dense desmoplasia. Surgery is the only effective treatment because mechanisms supporting tumor survival are unknown. We used single-cell RNA sequencing to characterize a patient-derived FLC xenograft model and identify therapeutic targets. Human FLC cells segregated into four discrete clusters that all expressed the oncogene Yes-associated protein 1 (YAP1). The two communities most enriched with cells coexpressing FLC markers [CD68, A-kinase anchoring protein 12 (AKAP12), cytokeratin 7, epithelial cell adhesion molecule (EPCAM), and carbamoyl palmitate synthase-1] also had the most cells expressing YAP1 and its proproliferative target genes (AREG and CCND1), suggesting these were proliferative FLC cell clusters. The other two clusters were enriched with cells expressing profibrotic YAP1 target genes, ACTA2, ELN, and COL1A1, indicating these were fibrogenic FLC cells. All clusters expressed the YAP1 target gene and mesothelial progenitor marker mesothelin, and many mesothelin-positive cells coexpressed albumin. Trajectory analysis predicted that the four FLC communities were derived from a single cell type transitioning among phenotypic states. After establishing a novel FLC cell line that harbored the DNAJB1-PRKACA fusion, YAP1 was inhibited, which significantly reduced expression of known YAP1 target genes as well as cell growth and migration. Thus, both FLC epithelial and stromal cells appear to arise from DNAJB1-PRKACA fusion in a YAP1-dependent liver mesothelial progenitor, identifying YAP1 as a target for FLC therapy.

2015-09-01·Journal of Biomedical Materials Research Part A3区 · 工程技术

Scaffold materials from glycosylated and PEGylated bovine serum albumin

3区 · 工程技术

Article

作者: Wu, Yonnie ; Choi, Young‐Suk ; David, Allan E. ; Wang, Kai ; Buschle‐Diller, Gisela

AbstractBovine serum albumin has been PEGylated and glycosylated to create mimetic materials for the extracellular matrix (ECM) with potential tissue engineering applications. Different surfaces for cell adhesion were achieved by crosslinking the initial albumin product and forming either a coating or a sponge‐like three‐dimensional morphology to mimic the mesh structure of natural ECM. The biocompatibility of the albumin matrix with mammalian cells was evaluated using cell culture assays with NIH 3T3 cells. The results indicated that glycoprotein composition and specific morphology of the assembly can improve the cell growth environment. These ECM mimetic structures might eventually be considered to serve as alternatives for the more expensive collagen and elastin based ECM substances currently in use in tissue engineering. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 2839–2846, 2015.

2015-07-01·Nan fang yi ke da xue xue bao = Journal of Southern Medical University

[Preparation of a decellularized scaffold derived from human liver tissue].

Article

作者: Liu, Wenyan ; Xiang, Junxi ; Wu, Wanquan ; Lü, Yi ; Liu, Xuemin ; Zheng, Xinglong

OBJECTIVETo develop a method for preparing a decellularized scaffold based on human liver tissue.METHODSA surgical specimen of the left lateral lobe of the liver was obtained from a patients with hepatic hemangioma. The decellularization process was performed by repeated freezing-thawing, sequential perfusion with 0.01% SDS, 0.1% SDS and 1% Triton X-100 through the portal vein, and sterilization with peracetic acid. L-02 cells were then engrafted onto the decellularized liver scaffold.RESULTSHE staining, DAPI staining and scanning electron microscopy all verified the absence of residual cellular components in the decellularized scaffold. The residual DNA content in the decellularized scaffolds was 25.3∓14.6 ng/mg (dry weight), which was less than 1% of the total DNA content in a fresh human liver. Immunohistochemistry demonstrated that type I and IV collagens, fibronectin and elastin were all retained in the scaffold. The engrafted L-02 cells survived well on the scaffold with active proliferation and expressed albumin and G6pc.CONCLUSIONIt is feasible to prepare decellularized scaffolds using surgical specimens of human liver, which can be a new approach to constructing a tissue-engineered liver for clinical purposes.