Article
作者: Wang, Hui ; Hua, Xianwu ; Fan, Huifen ; Jia, William ; Huang, Hongwei ; Liu, Lianxiao ; Wang, Xuewei ; Xu, Jiang ; Hu, Chunxia ; Lin, Kang ; Jia, Rui ; Liu, Xiaoke ; Li, Jing ; Cheng, Xinhua ; Yang, Guohuan ; Zhang, Qiu ; Zhao, Ronghua ; Hou, Fujun ; Yu, Zhibin ; Wang, Zejun ; Yang, Xiaoming ; Liu, Xiaohu ; Lu, Jia ; Liu, Fei ; Lu, Changrui ; Pan, Xinping ; Zhang, Yuntao ; Xu, Fangjingwei ; Cheng, Hang ; Li, Yuwei ; Cai, Rujie ; Ding, Jun ; Li, Xinguo
The coronavirus SARS-CoV-2 has mutated quickly and caused significant global damage. This study characterizes two mRNA vaccines ZSVG-02 (Delta) and ZSVG-02-O (Omicron BA.1), and associating heterologous prime-boost strategy following the prime of a most widely administrated inactivated whole-virus vaccine (BBIBP-CorV). The ZSVG-02-O induces neutralizing antibodies that effectively cross-react with Omicron subvariants. In naïve animals, ZSVG-02 or ZSVG-02-O induce humoral responses skewed to the vaccine's targeting strains, but cellular immune responses cross-react to all variants of concern (VOCs) tested. Following heterologous prime-boost regimes, animals present comparable neutralizing antibody levels and superior protection against Delta and Omicron BA.1variants. Single-boost only generated ancestral and omicron dual-responsive antibodies, probably by "recall" and "reshape" the prime immunity. New Omicron-specific antibody populations, however, appeared only following the second boost with ZSVG-02-O. Overall, our results support a heterologous boost with ZSVG-02-O, providing the best protection against current VOCs in inactivated virus vaccine-primed populations.
