(+)-Epicatechin

点击蓝字，关注我们 Volume 16· Issue 8 · August 2026 长按扫码 直接阅读 Review papers Gut-brain axis dysregulation in Parkinson's disease: Mechanisms linking microbiota to neuroinflammation and α-synuclein pathology Zhenxiong Zhao, Mengdie Dong, Zhen Mu, Zhencang Zheng, Zhengwei Zhang J. Pharm. Anal. 2026. 16(8) 101521 https://doi.org/10.1016/j.jpha.2025.101521 人类对帕金森病（PD）的认知正在被重塑—它不仅是大脑疾病，更与肠道密切相关。近年来，“肠-脑轴”理论揭示了肠道微生物如何通过神经、免疫和代谢途径影响大脑健康。研究表明，PD患者普遍存在肠道菌群失调，这可导致肠道屏障受损，产生“肠漏”，使细菌内毒素—脂多糖等有害物质进入血液，引发全身性炎症，继而诱发神经炎症，加速多巴胺能神经元退化。更关键的是，肠道菌群失调可能触发α-突触核蛋白在肠道神经系统中错误折叠和聚集，再经由迷走神经扩散至大脑，形成PD典型的病理蛋白团块——路易小体。这一“肠道起源”假说，为PD的早期干预提供了全新思路。基于肠-脑轴机制，益生菌、益生元、粪便菌群移植等微生态干预策略正在积极探索中，有望成为PD早期诊断和治疗的新方向。关注肠道健康，或许就是守护大脑的第一步。 Highlights This review supports the gut-first hypothesis, wherein α-synuclein pathology originates in the gut and propagates to brain. Gut dysbiosis induces neuroinflammation and dopaminergic neurodegeneration via metabolites and barrier disruption. This highlights the promise of microbiota-targeted interventions and gut-derived biomarkers for PD diagnosis and therapy. Advanced analytical approaches for endocannabinoids and endocannabinoid-like substances: Applications in liver disease research Jun-Yi Zhou, Yan-Qing Chen, Hai-Yu Zhao, Jian-Bo Wan J. Pharm. Anal. 2026. 16(8) 101546 https://doi.org/10.1016/j.jpha.2026.101546 内源性大麻素（eCBs）及其类似物是一类重要的脂质信号分子，主要通过与大麻素受体相互作用，在能量代谢、炎症调控及多种疾病发生发展过程中发挥关键作用。由于其在生物样本中含量极低、结构多样且易受基质干扰，建立高灵敏度、高选择性的分析方法是实现准确定量与机制研究的基础。 本文系统综述了近年来eCBs及其类似物在样品前处理策略与分析检测技术方面的研究进展，重点比较和评估了不同方法在灵敏度、选择性、稳定性及适用性方面的优势与局限。同时，本文进一步探讨了eCBs在肝脏脂质代谢调控中的功能及其参与相关病理过程的分子机制。本综述旨在为eCBs及其类似物的高效分析提供方法学参考，并为其作为潜在诊断生物标志物在基础医学研究与临床转化中的应用提供理论支持。 Highlights eCBs and eCB-like substances are key lipid mediators in physiological and pathological regulation. Recent advancements in sample preparation and analytical techniques for eCBs have been comprehensively summarized. Chemical derivatization strategies enhance MS signal intensity and stability, and enable isomer differentiation. The roles of eCBs in liver diseases and their involvement in pathological processes have been thoroughly explored. PKM2: A gatekeeper in macrophage metabolic reprogramming Ke Li, Mingyu Wu, Qiuyu Zhang, Jiabin Wu, Xianyi Ding, Weihua Xiao J. Pharm. Anal. 2026. 16(8) 101564 https://doi.org/10.1016/j.jpha.2026.101564 本综述系统梳理了丙酮酸激酶M2（PKM2）在巨噬细胞代谢重编程中的关键调控作用。在炎症及多器官损伤等病理过程中，巨噬细胞的功能命运在很大程度上受代谢状态所塑造。文章指出，PKM2不仅是糖酵解末端的关键酶，更是连接代谢调控与免疫反应的核心节点。其在二聚体与四聚体构象间的动态转换，决定了维持糖酵解代谢通量或入核参与转录调控（如协同HIF-1α等），进而推动炎症相关基因表达与代谢表型重塑。此外，文章总结了PKM2介导的代谢失衡在脓毒症、肝脏疾病、心血管疾病等多种跨器官疾病中的病理意义，并梳理了通过小分子激动剂或抑制剂干预PKM2构象及核转位的潜在药理策略。本研究为靶向PKM2进行代谢炎症性疾病的精准干预提供了新的理论基础与潜在方向。 Highlights Propose multi-level evidence for PKM2 as a key regulatory factor in macrophage glucose metabolism reprogramming. Analyze the regulatory mechanism of the dynamic equilibrium of PKM2 conformation. Map the cross-tissue disease spectrum of PKM2-mediated macrophage metabolic reprogramming. Evaluate the efficacy of chemical/natural modulators targeting PKM2. Propose the future development of intervention methods targeting PKM2 to regulate macrophage metabolic reprogramming. Exploring the potential of natural products in treating alopecia areata Baoli He, Yujia Weng, Peihua Luo, Zhifei Xu, Hao Yan, Bo Yang, Qiaojun He, Jiabin Lu, Xiaochun Yang J. Pharm. Anal. 2026. 16(8) 101539 https://doi.org/10.1016/j.jpha.2025.101539 斑秃是一种由毛囊免疫豁免破坏引发的自身免疫性脱发疾病，现有疗法存在疗效差异、复发等问题，亟需多靶点治疗新策略。本综述系统总结了天然产物（萜类、黄酮类、多酚类及复方）治疗斑秃的研究进展。其作用机制涵盖激活Wnt/β-catenin和PI3K-Akt通路，促进毛囊周期转换与细胞增殖；抑制JAK-STAT、TGF-β、NF-κB等炎症通路，改善免疫微环境，减轻毛囊损伤。但同时，天然产物面临活性成分复杂、生物利用度低及临床证据不足等挑战。未来需借助靶向递送、机制验证和高质量临床试验推动其转化应用。本综述为天然产物干预斑秃提供了系统的理论依据，并为新型多靶点药物开发提供参考。 Highlights Systematically elucidates the pathogenic mechanisms of alopecia areata. Provides novel insights to guide future research on natural products for alopecia areata. Identifies promising molecular targets for the treatment of alopecia areata. Multifunctional MOF composites for advanced drug identification and screening: Innovations, applications and challenges Min Wang, Yanjie Cheng, Manyao Zhang, Shuang Liu, Linyuan Luo, Xiang Han, Jingdi Chen, Zhining Xia, Hongli Luo J. Pharm. Anal. 2026. 16(8) 101543 https://doi.org/10.1016/j.jpha.2025.101543 药物识别与筛选是精准医学与新药研发的关键环节，传统方法受限于复杂生物基质干扰、痕量检测灵敏度不足及特异性欠佳等问题。金属有机框架（MOF）复合材料具有孔道可调、比表面积高及功能可模块化集成的显著优势。本文系统综述四类MOF复合材料（碳点-MOF、磁性纳米颗粒-MOF、生物大分子-MOF及共价有机框架-MOF）的设计策略与性能优势，并总结其在多模态传感平台（荧光、电化学、比色及表面增强拉曼散射）和高效筛选平台（离线配体垂钓与固定化酶微反应器）中的创新应用。此外，文章讨论该领域面临的结构稳定性、构效关系解析、生物相容性评估、复杂体系多药区分及规模化制备等挑战，并展望未来发展方向，旨在为新型药物识别与筛选技术的开发提供设计参考。 Highlights Outlined the development, classification, and characteristics of MOF composites. Detailed preparation strategies and performance advantages in four MOF composites. Presented multimodal sensing and screening platforms based on MOF composites. Progressed in various MOF composites for drug identification and screening. Analyzed the existing challenges and countermeasures. Designing lysate-based hydrogels for on-demand therapy Yang Lv, Siteng Tieng, Xiaoling Xu, Wei Chen J. Pharm. Anal. 2026. 16(8) 101514 https://doi.org/10.1016/j.jpha.2025.101514 水凝胶是通过物理或化学交联形成的三维亲水性网络。由于其结构上模拟了天然细胞外基质，并且具有可调节的药物递送能力，因此在生物医学工程领域备受关注。传统水凝胶主要作为药物包封的被动支架；然而，已有大量研究转向了整合从细胞或组织中提取的生物活性裂解物，这些裂解物富含生长因子、细胞因子和抗原成分，用于构建动态、多功能的平台。基于裂解物的水凝胶将裂解物衍生因子的内在生物活性与水凝胶基质提供的时空控制相结合。这种协同作用能够调节肿瘤微环境、免疫启动和组织再生。在本综述中，我们重点介绍了基于各类裂解物的水凝胶，这些水凝胶对整个生物工程领域具有显著的指导价值。该技术代表了设计理念和治疗策略的创新。通过提出开发未充分探索的裂解物来源和整合刺激响应性材料的策略，旨在推动基于裂解物的水凝胶成为精准肿瘤学的下一代平台。 Highlights Lysate hydrogels advance biomedicine by merging biological complexity with engineering precision for future therapies. The diverse bioactive factors in lysates drive therapies across immunotherapy, tissue regeneration, and oncology. Stable, biocompatible lysate hydrogels transform regenerative medicine, drug delivery, and immunoengineering. Original articles TCM-Agent: Advancing network pharmacology and herbal medicine discovery with LLM-based multi-agent systems Xiting Wang, Yuanrong Wang, Wenqing Dong, Shanshan Guo, Kai Wang, Shuangshuang He, Yuqi Wang, Haorui Li, Jian Lyu, Meng Liu, Lantian Zhang, Yinghao Zhu, Yiyuan Peng, Liantao Ma, Yu Li J. Pharm. Anal. 2026. 16(8) 101581 https://doi.org/10.1016/j.jpha.2026.101581 北京中医药大学DeepTCM实验室与北京大学医维矩阵实验室合作，研发出了面向网络药理学与中药研究的大语言模型多智能体系统——TCM-Agent，旨在破解传统中药"成分复杂、靶点众多、机制难解"的分析困境。该研究不同于当前依赖人工操作或单一自动化工具的传统流程，TCM-Agent 将大语言模型的语义理解、逻辑推理与任务编排能力引入中药研究全流程，通过多个智能体协同工作，让系统像资深药理学家一样，自主完成知识推理、数据分析、文献验证与可视化呈现。基于100项研究和563个问答对的评测结果显示，TCM-Agent在不同大语言模型（LLM）基座上表现稳定，并将传统分析时间由平均5.5小时缩短至约2.1分钟。这一工作为"如何让AI主导中药复杂机制解析"提供了前沿解决方案，并为中医药研究的智能化转型与标准化发展提供范式参考。 Highlights TCM-Agent is the first LLM-powered multi-agent system for autonomous network pharmacology and herbal medicine, integrating knowledge reasoning, interactive visualization, and literature validation. Multi-agent collaboration helps address key challenges such as heterogeneous data integration, analytical bias, and manual verification, supporting more standardized and evidence-based interpretations. Benchmarking across 100 validated studies demonstrates that TCM-Agent significantly outperforms existing methods in answer accuracy, literature retrieval precision, and computational efficiency. Leveraging an AI-agent architecture, TCM-Agent drastically reduces analysis time from hours to minutes for network pharmacology and herbal medicine tasks, while preserving high-quality results. Arctigenin discovered by high-throughput screening ameliorates doxorubicin-induced cardiotoxicity as a novel natural KEAP1-NRF2 inhibitor Xinyu Heng, Chao Ye, Shaohong Huang, Ke Zhang, Yankui Liu, Yueyue Wu, Ning Sun, Hai Yang J. Pharm. Anal. 2026. 16(8) 101606 https://doi.org/10.1016/j.jpha.2026.101606 阿霉素（DOX）是临床常用化疗药物，但其诱导的心脏毒性（DIC）严重制约应用，亟需开发低毒高效的心脏保护剂。本研究对含有960种化合物的天然产物库进行高通量筛选，发现亚洲草药牛蒡的活性成分牛蒡子苷元（ATG）具备改善DIC的潜力。体内外实验证实，ATG可显著减轻DOX诱导的细胞死亡，降低活性氧积累，抑制铁死亡，维持线粒体功能，同时改善小鼠心脏功能和心肌结构完整。机制层面，ATG直接靶向Kelch样ECH相关蛋白1（KEAP1）的602位丝氨酸，竞争性阻断KEAP1-核因子E2相关因子2（NRF2）相互作用，减少NRF2泛素化降解，促使NRF2入核激活抗氧化反应元件（ARE）通路，上调抗氧化和抗铁死亡相关基因表达。总之，ATG作为一种新型NRF2激活剂，为改善DIC提供全新候选药物。 Highlights Arctigenin (ATG) is identified as a cardioprotectant for doxorubicin (DOX)-induced cardiotoxicity (DIC). ATG attenuated DIC by inhibiting oxidative stress and ferroptosis both in vitro and in vivo. ATG suppressed oxidative stress and ferroptosis in cardiomyocytes by serving as natural Kelch-like ECH-associated protein 1 (KEAP1)-nuclear factor erythroid 2-related factor 2 (NRF2) inhibitor. Rapid and sensitive screening of carbonic anhydrase natural inhibitors in traditional Chinese medicine via a carbon quantum dot-affinity adsorption nanosystem Lingling Yang, Shuxian Zhang, Xiaoxuan Fan, Keshuai Liu, Rui Mai, Wangyan Zhao, Qiang Han, Yongjian Ai, Qionglin Liang, Xueqin Ma, Guoning Chen J. Pharm. Anal. 2026. 16(8) 101607 https://doi.org/10.1016/j.jpha.2026.101607 碳酸酐酶（CA）活性检测及抑制剂的筛选对药物研发至关重要。本研究建立了一种基于碳量子点（CDs）荧光传感探针结合靶向亲和识别模型的方法，用于CA的灵敏检测及其抑制剂筛选。该方法首先以CDs作为探针用于快速检测中药样品中潜在的CA抑制剂，然后通过靶向亲和策略精确捕获、分离并鉴定中药样品中具有抑制作用的活性成分，两种模型联用显著提高了筛选的准确性与重现性。经阳性和阴性对照药物验证后，应用于80种不同中药样品的筛选，发现木瓜中的熊果酸和齐墩果酸，以及桂枝中的顺式肉桂酸和反式肉桂酸是具有抑制CA的活性成分。最后采用等温滴定量热法、分子对接及动力学模拟验证了相互作用效果。该方法对于快速筛选CA抑制剂药物具有很大的潜力。 Highlights A method was developed for CA inhibitor screening based on carbon quantum dot fluorescence sensing and affinity technology. Ursolic acid, oleanolic acid, cis-cinnamic acid, and trans-cinnamic acid were identified as CA inhibitors. This method offers great potential for identifying enzyme inhibitors in TCM samples. Chemical characterization of aconite polysaccharide and its ameliorative effect on ulcerative colitis by regulating gut microbiota and THDCA-driven TGR5/cAMP/PKA/NLRP3 pathway Hao Cheng, Dandan Zhang, Jinlu Wu, Hui Feng, Yaochuan Zhou, Yan Wan, Yuzhu Tan, Wuwen Feng, Cheng Peng J. Pharm. Anal. 2026. 16(8) 101642 https://doi.org/10.1016/j.jpha.2026.101642 中药附子抗炎作用显著，其多糖成分的免疫调节潜力近年备受关注。本研究从附子中分离了一种以α-1,4-葡萄糖为主链、含多分支的多糖APA-1，并探究其改善溃疡性结肠炎（UC）的作用机制。肠道菌群测序发现，APA-1能显著富集Dubosiella、Lactobacillus等有益菌，并抑制Escherichia-Shigella等致病菌。代谢组学分析显示APA-1可有效提升菌群代谢物牛磺猪去氧胆酸（THDCA）水平。粪菌移植证实APA-1调节后的肠道菌群可传递其UC保护效应。进一步研究发现，THDCA通过激活G蛋白偶联胆汁酸受体1-环磷酸腺苷-蛋白激酶A信号轴（TGR5/cAMP/PKA）信号通路，抑制核苷酸结合结构域富含亮氨酸重复序列和含热蛋白结构域受体3（NLRP3）炎症小体的组装与白细胞介素-1β（IL-1β）的释放，从而减轻UC。本研究为开发附子多糖治疗UC的产品提供了理论依据。 Highlights APA-1 is a glucan composed of a (1→ 4)-linked glucose backbone. APA-1 ameliorates ulcerative colitis by remodeling gut microbiota. THDCA metabolized by gut microbiota ameliorates ulcerative colitis by targeting TGR5/cAMP/PKA/NLRP3 pathway. APA-1 ameliorates ulcerative colitis by regulating taurohyodeoxycholic acid-driven TGR5/cAMP/PKA/NLRP3 pathway. Multi-omics uncovers the critical role of ceramide-mediated Acinetobacter growth suppression in pressure injury healing under aging and malnutrition Zhichao Wang, Yan Zhang, Jinli Hou, Bingyu Lv, Xue Li, Li Gao, Xuemei Qin, Yuetao Liu J. Pharm. Anal. 2026. 16(8) 101587 https://doi.org/10.1016/j.jpha.2026.101587 衰老和营养不良（MN）是压力性损伤的重要易感因素，作为个体耐受性和敏感性的关键环节常共同延迟创面愈合。然而，其协同作用对创面愈合延迟的影响尚未充分阐明。本研究构建衰老-MN双重打击模型，整合随机森林、多组学分析及体内外验证，发现表皮层神经酰胺（Cer）缺乏促进不动杆菌（Acinetobacter）过度定植，继而扰乱花生四烯酸代谢，增强自噬并抑制WNT信号，最终削弱皮肤再生、延迟创面愈合。局部补充Cer可呈剂量依赖性抑制Acinetobacter，降低细菌负荷并加速创面愈合。该研究从病理机制中发现具有干预潜力的关键内源性代谢物，拓展了Cer的应用场景，为老年压力性损伤的“菌群-代谢物”靶向干预提供新依据，体现了内源性代谢物的功能再发现与应用拓展。 Highlights Malnutrition-aging double hit disrupts healing via Acinetobacter overgrowth and ceramide deficit. Ceramide-Acinetobacter-AA-autophagy/WNT axis occurs in epidermis. Ceramide deficit initiates inflammation/autophagy-WNT suppression. Ceramide supplement inhibits Acinetobacter colonization, promoting wound healing. Aptamer-based nanoparticle drug delivery system designed for targeting GPC3-positive liver cancer Hongxin Zhou, Shuangshuang Dong, Zhan Si, Hao Niu, Jianwen Mao, Yiran Chen, Yuhuan Li, Weizhong Wu J. Pharm. Anal. 2026. 16(8) 101614 https://doi.org/10.1016/j.jpha.2026.101614 肝细胞肝癌（HCC）占原发性肝癌的80%−90%。现有治疗手段缺乏肿瘤特异性，毒副作用突出。磷脂酰肌醇蛋白聚糖3（GPC3）在多数肝癌组织中高表达，是极具潜力的靶向标志物。本研究基于DNA折纸技术，将靶向GPC3的核酸适配体AP613-1 组装为DNA纳米结构mTriangle-AP613-1，借助噬菌粒-辅助噬菌体生物合成方案实现大批量制备，并利用脂质体包载药阿霉素（DOX）构建药物递送系统mTriangle-AP613-1-liposomes（DOX）。体外细胞实验证实递送系统可特异性识别GPC3阳性肝癌细胞，载药后抗肿瘤活性优于游离DOX。体内动物实验表明，该纳米载药体系可在24h内富集于肿瘤组织，显著抑制肿瘤生长，对心、肝、肾损伤轻微。本研究提供了一种可规模化生产、靶向高效、安全性良好的纳米递送平台，为GPC3阳性肝细胞癌精准靶向治疗提供新思路。 Highlights An aptamer-based nanoparticle drug delivery system targeting GPC3-positive tumors was developed. The nanostructure showed an improved KD of 15.2 ± 0.21nM. The nanoparticle drug delivery system inhibited GPC3-positive tumor growth effectively in vivo. Aptamer-functionalized nanostructures show great potential for precision oncology advancement. DT-TRAP enables accurate target recognition via simplified dosing and low-temperature incubation Hanqing Zhang, Wenjie Yuan, Yi Li, Chunyu Li, Yizhou Xie, Ning Wan, Haiping Hao, Hui Ye J. Pharm. Anal. 2026. 16(8) 101605 https://doi.org/10.1016/j.jpha.2026.101605 药物靶标鉴定是解析药物作用机制和推进新药研发的重要基础。针对现有化学蛋白质组学方法质谱资源消耗较大等问题，研究者在靶标响应可及性分析（TRAP）基础上，对药物剂量和孵育温度进行优化，建立了剂量–温度优化的DT-TRAP策略。研究表明简化的三剂量设计与较低的温度孵育能有效保持药物蛋白相互作用并降低非特异性背景，可稳定识别模型化合物的已知靶标PKM2和HSP90家族蛋白。将该策略应用于广谱药物staurosporine和阿司匹林后，DT-TRAP能够在复杂蛋白质组中有效筛选高可信候选靶标，并在肽段和赖氨酸位点水平提供与配体结合区域相对应的结构信息。该研究从实验参数优化的角度完善了TRAP技术体系，为提高化学蛋白质组学靶标发现的准确性、效率和可推广性提供了新的技术方案。 Highlights Rational optimization of dose and temperature improves target identification accuracy in TRAP. A simplified three-dose TRAP strategy distinguishes true targets while reducing experimental burden. Low-temperature incubation (4 °C) suppresses nonspecific interactions and reduces false positives. DT-TRAP identifies multiple targets of staurosporine and aspirin with peptide-level binding site resolution. DT-TRAP provides a robust workflow for accurate and efficient drug target discovery. Integrating mass spectrometry imaging and data-driven segmentation for spatial metabolic mapping of diabetic eye disease Shuohan Cheng, Shuo Wang, Tianfang Lan, Hongtao Jin, Zhi Zhou, Zhonghua Wang, Zeper Abliz J. Pharm. Anal. 2026. 16(8) 101596 https://doi.org/10.1016/j.jpha.2026.101596 本研究聚焦于糖尿病眼病（DED）的空间代谢异常及其干预机制，构建了融合AFADESI-MSI与高空间分辨率MALDI-MSI的双平台空间代谢组学研究策略。通过对糖尿病大鼠全眼矢状切片进行原位代谢成像，共注释135种代谢物，揭示了不同眼组织及视网膜层间显著的代谢异质性；进一步结合基于质谱特征的数据驱动分区，实现视网膜精细代谢区域的空间解析。研究发现，DED进程中伴随氨基酸、糖类、脂质、核苷酸及氧化还原代谢的区域特异性重塑。阿魏酸干预可改善多种异常代谢物水平及视网膜组织损伤，显示出潜在的眼组织保护作用。本研究为解析糖尿病眼病的空间代谢特征及评价药物干预效应提供了新的技术框架。 Highlights AFADESI-MSI and MALDI-MSI were used for mapping rat eye metabolic atlas. Regional metabolic heterogeneity in the rat eye was revealed. Identified and spatially resolved 39 key metabolites in diabetic eye disease eyes. Provided novel insights into diabetic eye disease pathology and therapeutic potential of ferulic acid. Diosgenin presents a novel role in promoting diabetic wound healing: A mechanism involving Sirt6/Nrf2-mediated inhibition of ferroptosis Ji-qi Wang, Lu Chen, Yu-zhe Lin, Xiu-zhi Zhang, Yi-tian Yu, Yi-han Lin, Li-jiang Han, Yi-yun Lv, Nai-feng Tian, Zhen Lin, Wei-jun Guo J. Pharm. Anal. 2026. 16(8) 101635 https://doi.org/10.1016/j.jpha.2026.101635 薯蓣皂素（DG）是一种源自野山药等传统中草药的甾体皂苷类活性成分。本研究首次揭示了DG通过激活沉默信息调节因子6（Sirt6）/核因子E2相关因子2（Nrf2）信号通路抑制铁死亡（Ferroptosis），从而促进糖尿病创面（DW）愈合的新机制。在高糖高脂（HGHF）微环境下，人脐静脉内皮细胞（HUVECs）发生由铁依赖性脂质过氧化驱动的铁死亡，导致细胞功能障碍；DG处理可显著逆转上述损伤，恢复线粒体膜电位，降低活性氧（ROS）和丙二醛（MDA）水平，并上调谷胱甘肽（GSH）及抗铁死亡蛋白GPX4、SLC7A11的表达。分子对接与细胞热位移实验证实DG可与Sirt6直接结合；DG通过促进Nrf2核转位、削弱Keap1-Nrf2相互作用，转录激活下游抗氧化基因。体内实验进一步表明，DG可加速糖尿病小鼠创面闭合，促进血管新生，并抑制创面组织铁死亡。总之，本研究阐明了DG通过Sirt6/Nrf2通路抑制铁死亡以促进糖尿病创面愈合的新机制，为糖尿病创面的天然药物治疗提供了理论依据与新策略。 Highlights HGHF induce ferroptosis in HUVECs in vitro and promote ferroptosis in diabetic wound tissues in vivo. Diosgenin inhibits HGHF-induced HUVECs ferroptosis and restores HUVECs functional impairment in vitro. Diosgenin exerts anti-ferroptotic effects by activating the Sirt6/Nrf2 signaling pathway. Diosgenin promotes diabetic wound healing in vivo in a Sirt6-dependent manner. Probucol inhibits palmitic acid-mediated renal calcium oxalate stone formation by targeting KLF5-PPARγ axis-mediated lipotoxicity Haotian Ren, Yutong Chen, Yang Li, Jingdong Zhang, Yi Shao, Bangxian Yu, Linguo Xie, Shiyong Qi, Rui Wang, Zhiqun Shang, Chunyu Liu J. Pharm. Anal. 2026. 16(8) 101575 https://doi.org/10.1016/j.jpha.2026.101575 泌尿系结石，尤其是草酸钙（CaOx）结石，是全球范围内常见且易复发的疾病。近年来，越来越多研究发现，代谢异常，特别是脂质代谢紊乱，与肾结石形成密切相关。本研究发现，饱和脂肪酸软脂酸（PA）在CaOx结石患者中显著升高，并可通过诱导肾小管上皮细胞脂毒性损伤，促进CaOx晶体粘附和沉积。通过高通量药物筛选及多维度实验验证，本研究发现传统降脂药物普罗布考（Pro）能够有效减轻PA诱导的肾小管损伤，并抑制CaOx晶体形成。进一步机制研究表明，PA可激活KLF5-PPARγ信号轴，促进异常脂质积聚、线粒体功能障碍及细胞损伤，而Pro可直接结合KLF5锌指结构域中的Arg440位点，抑制KLF5与PPARγ启动子的结合，从而降低PPARγ介导的脂毒性反应。本研究首次揭示了KLF5-PPARγ介导的脂毒性在CaOx结石形成中的关键作用，并发现Pro作为一种潜在干预药物，可通过靶向该信号通路减少结石形成。本研究为理解代谢异常驱动肾结石发生提供了新的理论依据，并为CaOx结石的精准防治提供了新的治疗策略。 Highlights High-throughput screening identifies probucol as a potent inhibitor of PA-mediated renal CaOx stone formation. RNA-seq shows probucol suppresses PA-induced PPARγ, revealing its pathogenic role in benign kidney disease for the first time. Probucol binds Arg440 in KLF5’s zinc finger, impairing its DNA binding and thereby reducing KLF5-driven PPARγ transcription. Probucol alleviates PA-induced lipotoxicity by targeting the KLF5-PPARγ axis, thereby reducing renal CaOx stone formation. Clinical validation confirms PA-associated upregulation of KLF5 and PPARγ in patients with renal CaOx stone. Short communications A facile antibody-mediated CMC platform for targeted screening of CCR1 ligands from Magnolia officinalis Ting Zhang, Shengjie You, Wenhao Chen, Hao Liu, Taiping He, Qiao Zhang, Xiaojuan Tian, Xiang Sun, Limei Liang, Suleixin Yang, Sanyin Zhang, Huachao Bin, Shilin Chen J. Pharm. Anal. 2026. 16(8) 101699 https://doi.org/10.1016/j.jpha.2026.101699 本研究借助Flag标签，成功构建M2抗体介导的C-C趋化因子受体1型（CCR1）靶向细胞膜色谱（Flag-CCR1/CMC）筛选平台，拓展了CMC固定化策略。利用该平台，首次从厚朴提取物中鉴定出和厚朴酚与厚朴酚为选择性CCR1拮抗剂。表面等离子体共振（SPR）及竞争结合实验表明，两者与CCR1均具有较强亲和力，且能阻断拮抗剂BX471结合。环磷酸腺苷（cAMP）积累实验和ONE-GO生物传感器显示，二者可逆转CCL3诱导的Gi信号抑制，效果与BX471相当。在脂多糖（LPS）诱导的RAW264.7巨噬细胞炎症模型中，和厚朴酚与厚朴酚显著抑制白介素-6（IL-6）和一氧化氮（NO）释放，并下调CCR1及下游蛋白激酶B磷酸化（p-AKT）、细胞外信号调节激酶1/2磷酸化（p-ERK1/2）蛋白表达。以上结果揭示了厚朴通过靶向CCR1发挥抗炎作用的新机制，该策略在针对其他G蛋白偶联受体（GPCR）配体的高通量筛选中亦具重要应用潜力。 Highlights A Flag-tag-based CMC platform was developed to enable highly specific and sensitive screening of CCR1 receptor antagonists. Magnolol and honokiol, two natural compounds derived from Magnolia officinalis, were identified as novel CCR1 antagonists. Competitive binding of magnolol, honokiol, and BX471 to CCR1 was confirmed by a newly designed Nile Reda probe. Both compounds showed potent anti-inflammatory effects in vitro/vivo, supporting therapeutic potential. This study established a high-throughput CMC-based platform to identify bioactive components in traditional medicine. Identification of lotus seeds stored over different periods by analyzing the changes in the chemical structure of starch Xi-Jun Lian, Ruo-Meng Zhang, Yu-Jia Shen, Xue-Xiao Cao, Qiu-Ping Wei, Chang-Jiang-Sheng Lai J. Pharm. Anal. 2026. 16(8) 101594 https://doi.org/10.1016/j.jpha.2026.101594 Highlights The wavenumber shift of lotus seed starch from 1019.6 cm−1 to 1016.3 cm−1 happens during storage. The resonance of C4 in lotus seed starch shifts to higher fields during storage. Intensities of diffraction angle at 2θ 11.40°/17.96° of lotus seed starch increases/decreases during storage. The single helical conformations of lotus seed starch transform into double ones. The crystallinity of lotus seed starch increases during storage. Unraveling the mechanism of Lianqiaobaidu powder in viral myocarditis: Complementary actions within cardiac cell types Na Tang, Shuqiao Xing, Ting Zhou, Wen-Hui Qi, Bingyan Wang, Chengwen Li, Wei Cao, Zhi-Jing Zhao, Yingfeng Lei, Xiao-Qiang Li J. Pharm. Anal. 2026. 16(8) 101595 https://doi.org/10.1016/j.jpha.2026.101595 Highlights Lianqiaobaidu powder alleviates cardiac dysfunction and injury in CVB3-infected mice. Nodakenin, isosakuranin, and epicatechin gallate are key LQBDP anti-VMC constituents. Nodakenin inhibits CVB3 replication in cardiac fibroblasts via the MEK pathway. Isosakuranin reduces inflammation in CVB3-infected macrophages via the RAGE pathway. Epicatechin gallate blocks CVB3-induced cardiomyocyte apoptosis via the TNFR pathway. Multicomponent coordination-driven self-assembled metallacycles as efficient photosensitizers for photodynamic therapy Jinping Zhang, Yali Hou, Qian Feng, Dengke Han, Qi Sun, Yuling Wu, Sida Liu, Dong Liu, Fei Xue, Jin Zhang, Jianing Wang, Xianglong Duan, Feng Liu, Mingming Zhang J. Pharm. Anal. 2026. 16(8) 101644 https://doi.org/10.1016/j.jpha.2026.101644 光动力疗法（PDT）因低毒、无耐药性优势成为肿瘤治疗研究热点，但现有光敏剂仍存在活性氧产率不足、功能单一等缺陷。本研究通过多组分配位自组装构筑3种新型金属环状光敏剂4a–4c，集成聚集诱导发光与电子受体特性，引入硫族元素调控电子耦合作用，其中含碲光敏剂4c单线态氧量子产率达0.86，性能远超商用玫瑰红。细胞与动物实验证实，4c光照下可高效诱导结肠癌细胞凋亡，有效抑制体内肿瘤生长，暗毒性与系统毒副作用极低，为高性能PDT光敏分子的设计提供了新路径。 Highlights Three metallacycles (4a–4c) were constructed via multicomponent coordination-driven self-assembly as enhanced PDT platforms. Cationic metallacycles promote preferential accumulation in tumor cells, improving PDT efficacy. In vitro/in vivo assays showed potent cytotoxicity against HCT116/SW480 cells under light, with negligible dark toxicity. This self-assembly strategy enables precise integration of functional motifs, offering a promising supramolecular approach for PDT advancement. 近期阅读： JPA文章推荐 | 南通大学孟国梁教授科研团队综述——调节性细胞死亡在动脉粥样硬化中的作用及研究进展 江南大学孙宁教授/吴可嘉副教授科研团队成果——基于DNA四面体纳米结构的EGFR/Ca²⁺双靶标高通量筛选平台用于抗心肌肥厚药物发现 期刊简介 Journal of Pharmaceutical Analysis（JPA,《药物分析学报（英文）》）创刊于2011年，由教育部主管，西安交通大学主办，是国内第一本有关药物分析的专业英文学术期刊，JPA 始终秉承服务国家重大战略需求、建设世界一流科技期刊的办刊宗旨，重点报道药物发现与药品全生命周期质量控制的新理论、新技术、新方法，临床精准用药，以及药物与生物、人工智能等交叉领域的技术方法方面的最新研究成果，为全球药物研发和药品质量控制提供高水平的国际学术交流平台，持续推动药物分析学科以及药学领域的快速发展。 JPA目前已组建一支以主编贺浪冲教授为核心的国际化的学术团队和专业的编辑出版团队，已实现编委国际化、稿源国际化、同行评议国际化、读者国际化和出版国际化。已被SCIE、PubMed、Scopus、DOAJ、中国科学引文数据库（CSCD）及中国科技论文与引文数据库（中国科技核心期刊）等多种重要国际和国内数据库和评价体系定为刊源。JPA连续8年入选“中国最具国际影响力学术期刊”。2019年入选“中国科技期刊卓越行动计划”重点期刊，2024年入选“中国科技期刊卓越行动计划二期”英文领军期刊。2025年影响因子 11.2，位于全球药理学和药学类学术期刊第15位（15/356），继续稳居于Q1区前列。2026年新锐期刊分区表1区，Top期刊。 收稿范围 药物分析新技术、新方法，分析药理学，药物代谢与递送，中药与天然药物，生物传感，可视化分析，生物功能分析，生物技术药物，药物分析装备，人工智能应用 收稿栏目 原创论文、综述、快报、展望、观点、新闻、社评等 期刊官网 https://www.journals.elsevier.com/journal-of-pharmaceutical-analysis 投稿网址 https://www.editorialmanager.com/jpa/Default.aspx  编辑 | 李   蕾  校对 | 朱丹丹 审核 | 王梦杰、马维娜 阅读原文 了解更多