OBJECTIVE:T o explore an approach to the reversal of multidrug resistance(MDR) of tumor cells.
METHODS:Antisense oligodeoxynucleotide (mdr-1-AS PS-ODN) was used.
RESULTS:A large amount of DNA fragments was found in mdr-1-AS PS-ODN treated K562/ADM cells and almost all the mdr-1+ K562/ADM cells underwent apoptosis.
CONCLUSION:mdr-1-AS PS-ODN could inhibit specifically mdr-1 gene expression, effectively reverse MDR of the tumor cells and promote ADM induced apoptosis of mdr-1+ K562/ADM.