The issue of Cervical cancer has received considerable critical attention. It was the leading cause of death in female cancer patients. Ginsenoside Rg5 has been implicated in the growth of human cancer cells, but the lack of research to determine the influence mechanism has been a significant challenge for many years. In this study, ginsenoside Rg5 was used to treat HeLa cells, and RT-qPCR, Western Blotting and RNA-seq were used to analyze the differentially expressed genes in HeLa cells to study the preliminary mechanism of ginsenoside Rg5 on cervical cancer. The results of RT-qPCR and Western Blotting were in agreement with RNA-seq analysis, which showed a down-regulation of proteins in the cell cycle pathway. These results all indicate that the expression of Minichromosome Maintenance Complex Component 6 (MCM6), Cyclin A2 (CCNA2), Cyclin-dependent kinase 6 (CDK6) and Cell division cycle 6 (CDC6) in HeLa cells treated with ginsenoside Rg5 is significantly reduced, with MCM6 exhibiting the most significant inhibitory effect, making it a potential target for the treatment of cervical cancer.

2025-04-01·FREE RADICAL BIOLOGY AND MEDICINE

Targeting LKB1–AMPK–SIRT1–induced autophagy and mitophagy pathways improves cerebrovascular homeostasis in APP/PS1 mice

Article

作者: Li, Hongrong ; Jiang, Yuning ; Li, Mengnan ; Kang, Ning ; Li, Yawen ; Kang, Tianyu ; Lu, Xuan ; Zhang, Runtao ; Wang, Tongxing ; Shen, Xiaogang ; Wu, Yiling ; Guo, Zhifang ; Hou, Yunlong

BACKGROUND:

Alzheimer's disease (AD) is the most common and severe degenerative disorder of the central nervous system in the elderly, profoundly impacting patients' quality of life. However, effective therapeutic agents for AD are still lacking. Bazi Bushen (BZBS) is a traditional Chinese herbal compound with potential neuroprotective effects, yet its underlying mechanisms remain poorly understood.

METHODS:

In this study, we utilized APP/PS1 transgenic mice to assess the therapeutic efficacy of BZBS. Initially, we evaluated the spatial learning and memory of the mice using the Barnes maze. The brain microcirculation was assessed through a small-animal ultrasound system, two-photon in vivo imaging, and micro-computed tomography angiography. Molecular, biochemical, and pathological analyses were conducted on brain tissues. Through network pharmacology, we identified potential intervention pathways and targets for BZBS in the treatment of AD, which we subsequently validated both in vivo and in vitro. Additionally, we employed molecular virtual docking screening and bio-layer interferometry to elucidate the direct interactions of ginsenoside Rg5 and ginsenoside Ro in BZBS with AMPK and LKB1 proteins.

RESULTS:

The BZBS intervention significantly enhanced spatial learning and memory in APP/PS1 mice while decreasing Aβ deposition. Furthermore, BZBS protected cerebrovascular homeostasis and mitigated neuroinflammation, as evidenced by decreased blood-brain barrier permeability, increased expression of tight-junction proteins, and restored cerebral blood flow. Mechanistically, ginsenosides Rg5 and Ro in BZBS directly bind to AMPK and LKB1 proteins, activating the LKB1-AMPK-SIRT1 signaling pathway, promoting autophagy and mitochondrial autophagy, and alleviating oxidative stress damage in endothelial cells.

CONCLUSIONS:

BZBS enhances autophagy-related activity, decreases Aβ deposition, and improves endothelial cell homeostasis through the activation of the LKB1-AMPK-SIRT1 signaling pathway, ultimately leading to improved cognitive function in mice with AD. This study highlights the importance of enhancing autophagic activity and maintaining cerebrovascular homeostasis in mitigating cognitive decline in AD, providing evidence and new insights into the application of compound medicines for treating age-related neurological disorders.

2025-03-01·JOURNAL OF SEPARATION SCIENCE

An Efficient Method to Separate the Main Components from Black Ginseng of Panax quinquefolium L. by Recycling and Consecutive High‐Speed Counter‐Current Chromatography Coupled With Evaporative Light Scattering Detection

Article

作者: Dong, Xiaodi ; Cao, Guiyun ; Park, Jeonghill ; Yu, Hongxia ; Wang, Zheng ; Sohail, Ammara ; Wang, Daijie ; Meng, Zhaoqing ; Sun, Huaming ; Qin, Xiuheng ; Van Le, Thi Hong ; Zhao, Yan

ABSTRACT:

Black ginseng (BG), a novel manufactured ginseng product, is gaining market attention due to its high polarity and similar structure constituents. In this work, the primary constituents of Panax quinquefolium L. BG, including ginsenosides and derivatives, were separated using high‐speed counter‐current chromatography in conjunction with evaporative light scattering detection by recycling and consecutive mode, as well as the elution‐extrusion method for continuous sample loading. Sample loading and mobile phase ratios were among the optimized primary separation parameters. Nine compounds, including ginsenoside Rg6 (1), 22(E)‐ginsenoside F4 (2), ginsenoside Rk3 (3), ginsenoside Rh4 (4), ginsenoside Rg3 (5), 20(R)‐ginsenoside Rg3 (6), (3R,9R,10R)‐panaxytriol (7), ginsenoside Rk1 (8), and ginsenoside Rg5 (9), were ultimately effectively isolated. Mass spectrometry and nuclear magnetic resonance spectrometry were used to investigate the structures. Based on our research, the developed process was a successful way to separate the primary ingredients from BG. Purification of additional comparable high‐polarity chemicals from complicated natural products might be accomplished using the stated methodology.

项与 Ginsenoside Rg5 相关的新闻（医药）

2024-01-26

·PRNewswire

Three Journal of Pharmaceutical Analysis Studies Explore the Use of Traditional Chinese Medicine for Various Diseases

XI'AN, China, Jan. 26, 2024 /PRNewswire/ -- Despite demonstrating significant clinical efficacy against various diseases, the widespread use of Traditional Chinese Medicine (TCM) is still limited. This is mainly due to the complexity in their formulation and lack of sufficient pharmacological and safety-related data. To address this gap, a new issue of the Journal of Pharmaceutical Analysis presents three independent studies which assessed the potency of TCM-based compounds for various human diseases, along with their molecular mechanisms of action. Continue Reading Latest research unveils the potential of Traditional Chinese Medicine compounds for the treatment of human diseases The accumulation of excess fat in the liver leads to non-alcoholic fatty liver disease (NAFLD). Oridonin (ORI), a TCM derived from the Chinese herb Rabdosia rubescens, exhibits anti-inflammatory effects. An article available online on 21 August 2023 and published in Volume 13, Issue 11 of the journal in November 2023, now reveals that ORI regulates lipid homeostasis in the liver by maintaining the balance between triglyceride (TG) and phosphatidylethanolamine (PE), through modulation of adipose triglyceride lipase (ATGL) and ethanolamine phosphotransferase 1 (EPT1) expression via the liver X receptor alpha (LXRα) signaling pathway. "When the TG-PE lipid balance is disturbed, the seesaw tilts towards TG, increasing the risk of NAFLD. Restoring lipid homeostasis using compounds like oridonin can alleviate lipid accumulation and liver cytotoxicity," explains Professor Lan Tang. Tumor heterogeneity compromises the efficacy of radiotherapy for treating lung cancer. Radiosensitizers help prime tumor cells and improve their response to radiation. Now, in an article available online on 7 June 2023 and published in Volume 13, Issue 11 of the journal in November 2023, researchers found that ginsenoside Rg5 induced cell-cycle arrest and enhanced radiation-induced cell death. Ginsenoside Rg5 interacts with heat shock protein alpha (HSP90α) with high affinity and reduces the binding between HSP90 and cell division cycle 37 (CDC37), promoting HSP90-CDC37 client protein degradation. "Ginsenoside Rg5 can be potentially developed into a new drug for improving the sensitivity of lung cancer and other types of tumors to radiation therapy," the authors explain. Diabetic retinopathy (DR) is characterized by the activation of oxidative stress pathways in response to high glucose. In a study available online on 12 May 2023 and published in Volume 13, Issue 11 of the journal in November 2023, researchers synthesized a polymeric drug complex by combining a natural flavonoid antioxidant known as dihydromyricetin (DMY) with iron (Fe) ions in nano-coordinated polymer particles (NCPs). Fe-DMY NCPs can alleviate glucose-induced oxidative damage and reverse the pathological features of DR by decreasing the expression of key proteins involved in microvascular dysfunction. Fe-DMY NCPs could also inhibit the activation of Poldip2-Nox4-H2O2 signaling pathway and down-regulate important vascular function indicators such as VCAM-1, HIF-1α, and VEGF. Explaining its applications, the authors say, "We report for the first time the synthesis and validation of ultra-small Fe-DMY NCPs. Fe-DMY complexes bear the potential to alleviate the impact of DR on vision, thus improving the quality of life of affected individuals." These studies provide a scientific basis for the development of TCM-based compounds into effective targeted therapies for various diseases. Reference Titles of original papers: Oridonin restores hepatic lipid homeostasis in an LXRa-ATGL/EPT1 axis-dependent manner Ginsenoside Rg5 enhances the radiosensitivity of lung adenocarcinoma via reducing HSP90-CDC37 interaction and promoting client protein degradation Nanoscale coordination polymer Fe-DMY downregulating Poldip2-Nox4-H2O2 pathway and alleviating diabetic retinopathy Journal name: Journal of Pharmaceutical Analysis DOI: Media contact Mengjie Wang [email protected] Xi'an, China +86-(0)29-88960092 SOURCE Journal of Pharmaceutical Analysis

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适应症	最高研发状态	国家/地区	公司	日期
单纯疱疹病毒性脑炎	临床前	韩国	Korea Institute of Oriental Medicine	2024-07-01
胶质母细胞瘤	临床前	中国	中国医科大学附属第一医院	2024-07-01
心脏病	临床前	中国	四川省肿瘤医院（四川省第二人民医院四川省癌症防治中心）	2024-05-24
非酒精性脂肪性肝炎	临床前	中国	Northwest University (China)	2024-02-01