3区 · 医学
Article
作者: Muñoz-Alía, Miguel Á ; Tesfay, Mulu ; Lathrum, Chase ; Kyratsous, Christos A ; Zhang, Lianwen ; Carey, Timothy ; Lee, Rachael M ; Petro, Christopher D ; Vandergaast, Rianna ; Peng, Kah-Whye ; Narjari, Riya ; Reiter, Samantha ; Suksanpaisan, Lukkana ; Sakuma, Toshie ; Ziegler, Christopher ; Ramachandran, Sabarinathan ; Graham, Melanie L ; Balakrishnan, Baskar ; Haselton, Michelle ; Baum, Alina ; Gnanadurai, Clement ; Waniger, Scott ; Russell, Stephen J ; Lech, Patrycja ; Packiriswamy, Nandakumar ; Sevola, Kara ; Krotova, Karina ; Russell, Luke ; Recker, Jordan ; Janecek, Jody L
An orally active vaccine capable of boosting SARS-CoV-2 immune responses in previously infected or vaccinated individuals would help efforts to achieve and sustain herd immunity. Unlike mRNA-loaded lipid nanoparticles and recombinant replication-defective adenoviruses, replicating vesicular stomatitis viruses with SARS-CoV-2 spike glycoproteins (VSV-SARS2) were poorly immunogenic after intramuscular administration in clinical trials. Here, by G protein trans-complementation, we generated VSV-SARS2(+G) virions with expanded target cell tropism. Compared to parental VSV-SARS2, G-supplemented viruses were orally active in virus-naive and vaccine-primed cynomolgus macaques, powerfully boosting SARS-CoV-2 neutralizing antibody titers. Clinical testing of this oral VSV-SARS2(+G) vaccine is planned.