Isoxsuprine Hydrochloride

The screening of drugs in plasma and urine often requires initial extraction (such as liquid–liquid extraction and solid‐phase extraction) before the samples are submitted to instrumental analyses. These extraction procedures are often laborious and time‐consuming. In this manuscript, a high‐throughput automated assay based on liquid chromatography–high‐resolution mass spectrometry (LC–HRMS) suitable for use as an initial testing procedure covering multiple classes of compounds prohibited in horse racing is described. The assay requires a 600‐μL plasma aliquot, which is subjected to solid phase extraction (SPE) using OASIS HLB 96‐well SPE with Biotage Extrahera system, evaporation, and reconstitution in a 96‐well collection plate. LC–HRMS analyses were carried out on a Thermo Q‐Exactive Mass spectrometer coupled with Thermo UHPLC system equipped with Thermo Accela ALS 2.4.0 autosampler linked to ACE Excel column. Drug targets were detected by retention time and accurate mass, with a mass tolerance window of 5 ppm in positive and negative ionization mode. The screening method was validated for over 300 drug targets in a 13‐min run. Validation data including sensitivity, specificity, extraction recovery, and precision are presented. As the method employs full‐scan mass spectrometry, unlimited number of drug targets can theoretically be incorporated into this method.

The present study was conducted to determine the clinical effects of isoxsuprine hydrochloride (IP) administered prior to elective and emergency cesarean operations on the mother and newborn. The study was carried out on 40 pregnant Awassi sheep aged 2 years, divided into two operation groups: elective cesarean and emergency cesarean. From these groups, a total of four groups were formed: Group I (n = 10), which received 5 mL saline solution 45 min before the cesarean operation; Group II (n = 10), which received 0.6 mg/kg IP 45 min before the cesarean operation; Group III (n = 10), which received 16 mg dexamethasone 24 h before the cesarean operation; and Group IV (n = 10), which received 16 mg dexamethasone 24 h before and 0.6 mg/kg IP 45 min before the cesarean operation. Preoperative Doppler ultrasound measurements were performed on the uterine artery, umbilical artery, fetal aorta, ductus venosus, fetal vena cava, and placentome arteries. Both preoperative and postoperative oxidative stress parameters, as well as postoperative blood gas and APGAR parameters, were evaluated. In the groups administered isoxsuprine, PI and RI values of the measured vessels decreased, while PSV, EDV, and A values increased significantly (p < 0.001). Blood gas parameters such as pH, pO₂, and HCO₃ levels were higher, whereas pCO₂ levels were lower in the isoxsuprine-treated groups. Lambs born to the isoxsuprine-treated groups exhibited higher APGAR scores (p < 0.05). Furthermore, antioxidant capacities (TAS) were higher, and oxidative stress levels (TOS, OSI) were lower in lambs from the isoxsuprine-treated groups (p < 0.001). In conclusion, isoxsuprine administration improved maternal and fetal circulation, enhancing blood flow and positively affecting offspring viability.