A method for the determination of Oxiracetam for Injection by HPLC was established.Chromatog. was performed on the Ultimate C18 column (4.6 mm × 250 mm, 5 μm), using methanol-water (5:95) as the mobile phase under the detection wavelength of 214 nm, and the flow rate was 1.0 mL/min-1.The linear range of oxiracetam was 0.0404-0.3232 mg/mL-1 (r = 0.9998).The average recovery rate was 99.6% (RSD = 1.0%).The method is simple and accurate with good reproducibility, which can be used for the quality control of Oxiracetam for Injection.