Objective: To determine the content of Adenosine in LIFEI Tablets by HPLC.Method: Waters Spherisorb C18(4.6 mm × 250 mm, 5 μm) column was used.The mobile phase was methanol-0.05 mol/L KH2PO4 (11: 89), and UV detection wavelength was 260 nm.Adenosine showed a good linear relationship in the range of 14-420 ng (r = 0.9997, n = 6).The average recovery was 102.3%, and RSD was 1.48% (n=5).Conclusion: The established method is accurate, reliable and specific.The results are stable with good reproducibility.The quality of the tablets can be controlled by the method.