Delving into the Latest Updates on VIB-VUB Center for Structural Biology with Synapse

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疾病领域	数量

肿瘤	1

排名前五的药物类型	数量

纳米抗体	1

关联

3

项与 VIB-VUB Center for Structural Biology 相关的药物

Cortivazol

可的伐唑

靶点

GR

作用机制

GR激动剂

在研机构-

原研机构-

在研适应症

关节疾病

非在研适应症

炎症

最高研发阶段批准上市

首次获批国家/地区-

首次获批日期-

Nanobodies(VIB)

靶点-

作用机制-

在研机构

VIB-VUB Center for Structural Biology

原研机构

VIB-VUB Center for Structural Biology

在研适应症

肿瘤

非在研适应症-

最高研发阶段临床前

首次获批国家/地区-

首次获批日期-

AZD-2906

靶点

GR

作用机制

GR调节剂

在研机构

AstraZeneca PLC

原研机构

AstraZeneca PLC

在研适应症

炎症

非在研适应症-

最高研发阶段临床前

首次获批国家/地区-

首次获批日期-

100 项与 VIB-VUB Center for Structural Biology 相关的临床结果

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0 项与 VIB-VUB Center for Structural Biology 相关的专利（医药）

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71

项与 VIB-VUB Center for Structural Biology 相关的文献（医药）

2025-06-01·Current Opinion in Structural Biology

Allosteric modulation of protein–protein interactions in signal transduction with Nanobodies

Review

作者: Steyaert, Jan ; Pardon, Els ; Wohlkönig, Alex

Nanobodies (Nbs), the variable domains of heavy-chain only antibodies that naturally occur in camelids, are exquisite molecular tools to stabilize dynamic proteins in unique functional conformations. Recent developments in Nb discovery allow to select allosteric Nbs that perturb the distribution of conformational ensembles of protein complexes that mediate signaling, leading to the allosteric modulation of the signals they transmit. Evidence is also accumulating that such conformational specific Nbs do not stabilize new conformational states but rather change the distribution of existing states to allosterically induce transitions, imprinted by the natural ligands of the system.

2025-06-01·STAR Protocols

Protocol for real-time glycolytic monitoring in mammalian cells using confocal microscopy and HYlight, a biosensor for fructose 1,6-bisphosphate

Article

作者: Baeyens, Nicolas ; Messens, Joris ; Ezeriņa, Daria ; Pérez-Chávez, Israel ; Gurzov, Esteban N

Here, we present a protocol for a glycolytic stress test using HYlight, a fluorescent protein-based biosensor that specifically binds fructose 1,6-bisphosphate (FBP), a product of the rate-limiting step of glycolysis. We describe steps for HYlight delivery into cells, real-time imaging by confocal microscopy, and data analysis. Compared with other techniques, HYlight allows glycolytic activity assessment in intact cells with single-cell resolution. This protocol can be applied to studies in which glycolysis might play a role, such as cancer or diabetes research. For complete details on the use and execution of this protocol, please refer to Pérez-Chávez et al.¹.

2025-04-18·Science Advances

Molecular basis of pyruvate transport and inhibition of the human mitochondrial pyruvate carrier

Article

作者: Stanik, Klaudia ; Cooper, Lee ; Pardon, Els ; Tavoulari, Sotiria ; Ding, Shujing ; Ruprecht, Jonathan J. ; Sowton, Alice P. ; Palmer, Shane M. ; Forrest, Lucy R. ; Paris, Giulia ; Kunji, Edmund R. S. ; Leone, Vanessa ; Hardwick, Steven W. ; Sichrovsky, Maximilian ; King, Martin S. ; Jones, Scott A. ; Chirgadze, Dimitri Y. ; Dionysopoulou, Mariangela ; Fearnley, Ian M. ; Lacabanne, Denis ; Steyaert, Jan ; Rana, Jessica J.

The mitochondrial pyruvate carrier transports pyruvate, produced by glycolysis from sugar molecules, into the mitochondrial matrix, as a crucial transport step in eukaryotic energy metabolism. The carrier is a drug target for the treatment of cancers, diabetes mellitus, neurodegeneration, and metabolic dysfunction–associated steatotic liver disease. We have solved the structure of the human MPC1L/MPC2 heterodimer in the inward- and outward-open states by cryo–electron microscopy, revealing its alternating access rocker-switch mechanism. The carrier has a central binding site for pyruvate, which contains an essential lysine and histidine residue, important for its ΔpH-dependent transport mechanism. We have also determined the binding poses of three chemically distinct inhibitor classes, which exploit the same binding site in the outward-open state by mimicking pyruvate interactions and by using aromatic stacking interactions.

100 项与 VIB-VUB Center for Structural Biology 相关的药物交易

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100 项与 VIB-VUB Center for Structural Biology 相关的转化医学

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