Comparison of the efficacy of single vs. divided dose of gonadotropin-releasing hormone agonist (GNRHa) as a trigger in overresponding patients undergoing intracytoplasmic sperm injection (ICSI)

开始日期2024-06-09

申办/合作机构

Avicenna Research Institute

NCT05019287

/ Completed临床1/2期IIT

Safety and Efficacy Study of Allogeneic Human Menstrual Blood Stem Cells Secretome to Treat Severe Covid-19 Patients, Clinical Trial Phase I&II

In this randomized controlled trial (RCT), severe cases of COVID-19 infection will be treated with secretome of menstrual blood stem cells. The improvement in the clinical, laboratory and radiological manifestations will be evaluated in treated patients compared with the control group.

开始日期2021-04-17

申办/合作机构

Avicenna Research Institute

[+1]

IRCT20200421047152N1

/ RecruitingN/AIIT

Evaluating the effect of sequential day3/ day5 embryo transfer on Improvement of pregnancy rate in patients with repeated implantation failure

开始日期2020-06-21

申办/合作机构

Avicenna Research Institute

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项与 Avicenna Research Institute 相关的文献（医药）

2025-08-01·Biosensors and Bioelectronics

A dual-Color closed bipolar electrochemiluminescence platform for visual simultaneous diagnosis of two pluripotency markers in cancer patients’ urine

Article

作者: Rabbani, Hodjattallah ; Xu, Guobao ; Hosseini, Morteza ; Arab, Nastaran ; Sadeghi, Niloufar

Here, for the first time, we introduce a simple yet effective one-step visual approach for the concurrent detection of octamer-binding transcription factor 4 (Oct-4) and Nanog, two pivotal homeobox genes involved in regulating pluripotency in both stem cells and certain cancer cells, within human urine samples. We utilized a closed bipolar electrochemistry system to enable the electrochemiluminescence (ECL) detection of pluripotency biomarkers. This was accomplished by capturing the ECL of Ru(bpy)₃²⁺ loaded into mesoporous SiO₂ nanoparticles and luminol incorporated into luminol@MIL-53 on the upper and lower anodic poles of BPEs in separate chambers of a bipolar cell using a digital camera. An identical electric potential was applied across the bipolar electrodes, driving the reduction of thionine acetate at the cathodic poles and thereby facilitating simultaneous light emission of the luminophores. Moreover, the incorporation of electroactive Ti₃C₂T_x MXene-TiO₂ nanosheets catalyzed the electro-oxidation of co-reactants within both the Ru(bpy)₃²⁺/TPrA and luminol/H₂O₂ systems, thereby enhancing the signal-to-background ratio and eliciting a significantly amplified visual ECL response. Under optimized conditions, the immunosensor displayed a linear response across a broad range of 100 pg mL^-1 to 400 ng/mL for Nanog, with a detection limit of 21.58 pg mL^-1, and a linear response from 200 pg mL^-1 to 400 ng/mL with a detection limit of 106.24 pg mL^-1 for Oct-4. This study supports the immunosensor's potential for broader clinical application, where its high specificity and sensitivity could offer significant benefits in cancer diagnostics and stem cell research.

2025-06-01·Reproductive Biology

Increased circulating T helper 17 (TH17) cells and endometrial tissue IL-17-producing cells in patients with endometriosis compared with non-endometriotic subjects

Article

作者: Delbandi, Ali-Akbar ; Farhangnia, Pooya ; Mahmoudi, Mahmoud ; Zarnani, Amir-Hassan ; Mohammadi, Tahereh ; Shervin, Adel

Endometriosis, an inflammatory disease, is characterized by the aberrant presence of endometrial tissues at ectopic locations. Accumulating evidence suggests that inflammatory cells, such as interleukin-17 (IL-17)-producing cells, may be involved in the pathogenesis of endometriosis. This investigation assessed the frequency of IL-17A (commonly known as IL-17)-producing cells in peripheral blood mononuclear cells (PBMCs), ectopic, and eutopic endometrial tissues in patients with endometriosis compared to non-endometriotic subjects. PBMCs, ectopic, and eutopic endometrial tissues were collected from 23 patients with endometriosis. PBMCs and endometrial tissues from 20 non-endometriotic women were used as the control group. The frequency of T helper 17 (T_H17) lymphocytes in PBMCs was assessed using flow cytometry, and the expression level of IL-17 in eutopic and ectopic endometrial tissues was evaluated through immunohistochemistry. The percentage of T_H17 and IL-17-producing lymphocytes was significantly higher in the PBMCs of patients with endometriosis compared to non-endometriotic subjects (P < 0.01 and P < 0.001, respectively). The expression of IL-17 protein in ectopic (P < 0.001) and eutopic (P < 0.05) endometrial tissues of patients with endometriosis increased compared to controls' endometrial tissue. Furthermore, the eutopic endometrium of patients with endometriosis showed a higher expression of IL-17 protein than the eutopic endometrial tissue of control subjects (P < 0.05). The findings suggest that the higher frequency of IL-17-producing cells in the PBMCs and endometrial tissues of patients with endometriosis contributes to the pathogenesis of endometriosis.

2025-05-01·Cryoletters

Efficient procedure for human adipose tissue cryopreservation without specialized freezing equipment

Article

作者: Heidari, Banafsheh ; Golshahi, Hannaneh ; Mehdi Tabaie, Seyed ; Soltanghoraei, Haleh ; Soltani, Azadeh ; Jafary, Hanieh ; Shams, Soroosh

BACKGROUND: Adipose tissue grafting is one of the reconstruction methods for damaged tissue repair. OBJECTIVE: To develop a convenient procedure for human adipose tissue cryopreservation without any special equipment. MATERIALS AND METHODS: Adipose tissues were frozen using different combinations of permeating and non-permeating cryoprotectants at a cooling rate of −1°C/min and stored at −20°C for 1, 3, 6 and 9 months. Histo‐morphological characteristics, mitochondrial activity, oil ratio (OR) index, survival and differentiation potential of mesenchymal stem cells of thawed adipose tissue were evaluated. RESULTS: The most damage or degeneration and OR indices of adipose tissues were detected in phosphate‐buffered saline without any cryoprotectant at 1, 3, 6, and 9 months after cryopreservation (P≤0.05). The best protection of adipose tissue against freezing damage was observed when using a solution of 0.5 M DMSO + 9% FBS + 0.2 M trehalose (P≤0.05). Similarly, mitochondrial activities of thawed adipose tissues were the highest in the 0.5 M DMSO + 9% FBS + 0.2 M trehalose, but lowest in the phosphate‐buffered saline. There was no difference in the stemness and differentiation potential of adipose tissue‐derived mesenchymal stem cells among different cryopreservation treatments. CONCLUSION: The combination of 0.5 M DMSO, 9% FBS and 0.2 M trehalose has the best protection for human adipose tissue during cryopreservation.

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