In this article, we introduced the optimization of high d. fermentation process of Streptococcus thermophilus.Starting with the optimization of culture medium, the pros and cons of single factor anal. method and orthogonal testing method were discussed.Then the optimization of culture condition and fermentation process such as the pH, temperature, agitator speed, inoculating quantity, and incubation time was described.Finally, the post-treatment process of bacteria including cooling treatment, ultrasound treatment, as well as centrifugation and lyophilization was introduced.