Quality standard of Zhonggan tablet was improved.Aristolochic acid A in Zhonggan tablet was identified by TLC, and gentiopicroside level in Zhonggan tablet was detected by HPLC.The separation was carried out on a Hyperisil ODS2 column (4.6 mm × 250 mm, 5 μm), using methanol-water-acetic acid (25:74:1) as mobile phase.The flow rate was 1.0 mL/min-1, detection wavelength was 270 nm, and column temperature was room temperatureAristolochic acid A was not identified by TLC in Zhonggan tablet.The linear range for gentiopicroside was 0.23-5.97 μg (r = 0.9998), average recovery was 99.3% with RSD 0.99% (n = 5).This method is simple, accurate, sensitive and reproducible, which is available for the quality control of Zhonggan tablets.