To determine the content of polysaccharide in Guiyang com. available Radix pseudostellariae. The polysaccharides were extracted with hot water-ethanol deposit method. The assay of polysaccharides was measured with UV-Vis method by the anthrone-sulfuric acid using glucose as the standard in the 626 nm wavelength. The content of polysaccharide in Radix pseudostellariae from Fukangtang Chinese and Western Pharmacy was the highest, but the content from Chinese crude drug wholesale department was the lowest. The contents of polysaccharides in Radix pseudostellariae from Fukangtang Chinese and Western Pharmacy, Beijing Tongrentang Guiyang pharmacy, Renhe special local products store, Guiyang l- Huaguoyuan Chinese crude drug wholesale market, Taisheng Wuying Chinese crude drug store, Sheng sheng Pharmacy, Baihe Chinese and Western Pharmacy, Tongjitang linkage Pharmacy, Chinese crude drug wholesale department were 18.43%, 17.48%, 15.15%, 13.65%, 12.52%, 12.13%, 11.60%, 10.23%, 9.74% , resp. The contents of polysaccharide in Guiyang com. available Radix pseudostellariae were different significantly, which may affect its action.