Objective:To establish an HPLC method for determining trace genotoxic impurities in furosemide and its preparationsMethods:The chromatog. column was filled with octadecylsilane bonded silica gel, with phosphoric acid solution and acetonitrile as mobile phase, and the detection wavelength was 270 nm.Results:The known impurities in the pharmacopoeia did not interfere with the detection.The detection limit of impurities was 12 ng·mL∼(-1).The linearity between 0.04-4.088 μg·mL∼(-1) was good, r∼2=1.000.The average recoveries of furosemide and its preparations were 99.8%(RSD=2.8%), 98.9%(RSD=0.9%)and 100.9%(RSD=2.4%).There were 0.003%-0.004% furfural detected in furosemide and its com. preparationsConclusion:This method has high specificity, sensitivity and good accuracy, and can be used to detect furfural, a trace gene toxin impurity in furosemide and its preparationsThis method ensures the efficacy and safety of furosemide preparations