The quality standard for Danggui blood tonic oral solution was established. Radix angelica sinensis, Rhizoma Chuanxiong, Glycyrrhiza uralensis and Astragalus membranaceus were identified by TLC, the content of paeoniflorin was determined by HPLC. Radix angelica sinensis, Rhicoma Chuanxiong, Glycyrrhiza uralensis and Astragalus membranaceus could be detected by TLC, the linear range was 0.12-3.05 μg for paeoniflorin, the average recovery was 98.2%, RSD 1.1%. The method is simple, feasible and reproducible, and it provides a method for quality control of Danggui blood tonic oral solution