Xinjiang Yinduolan Uighur Medicine Co., Ltd.

The effect of the gingerol on the melanogenesis in melanoma B16 cells was studied.Melanoma cells were cultured with gingerol at 12.5, 25.0, 50.0, 100.0, 200.0 μmol·L^-1 and pos. control drug hydroquinone, resp., using Dulbecco's modified eagle's medium (DMEM) as the blank control group.The cell proliferation was measured by Me thiazolyltet tetrazolium (MTT) colorimetric assay.The tyrosinase activity and melanin content were measured by colorimetry assay.Gingerol at different concentrations had inhibitory effect on B16 cell proliferation compared with the blank control group (P < 0.05 or P < 0.01), the inhibition rate being more than 48% at the dosage of 200.0 μmol · L^-1.Tyrosinase activity was inhibited significantly compared with blank control group (P < 0.05 or P < 0.01), the inhibition rate being up to 50% at the dosage of 200 μmol·L^-1.Melanin content was also decreased at all levels of gingerol compared with blank control group (P < 0.05 or P < 0.01), but not in a dose-dependent manner.The inhibition rate of melanin content reached the plateau at gingerol levels greater than 25.0 μmol · L^-1.Gingerol can inhibit the cell proliferation, tyrosinase activity and decrease melanin synthesis in certain range of concentrations

The aim of this study is to establish quality standard of Jinsuokunduer tablets.Cyperus tuber, Olibanum and Pepper were identified resp. by TLC, the contents of costunolide and dehydro-α-curcumene were determined by HPLC.The characteristic identification by TLC was distinct and highly specific.Costunolide was linear between 0.03∼0.07 mg/mL (r=0.9995), and the average recovery was 99.31% with RSD of 0.62%.Dehydro-α-curcumene was linear between 0.06∼0.14mg/mL(r=1), and the average recovery was 99.33% with RSD of 0.69%.The method is reliable, accurate and specific, and it can be used for quality control of this preparation

A review.The mechanism of melanogenesis is closely related with melanocytes, keratinocytes, lymphocytes and neurocytes; melanogenesis is also connected with the occurrence of dermatosis such as vitiligo.The paper reviews the relationship between the mechanism of melanogenesis and the related melanocytes, keratinocytes, T lymphocytes and neurocytes, and proves a theor. basis for further study.