Objective: To study the pharmacol. effects and mechanism of Renshen Baizhu Zaoren Granules on invigorating spleen and nourishing heart, quieting the heart and tranquilizing mind, improving sleep function. Methods: The mice model of heart-spleen deficiency syndrome was established by weight-bearing swimming method combined with drugs induction, and the effects of Renshen Baizhu Zaoren Granules on body weight, intestinal propulsion, tail suspension immobility, swimming time, sleep behavior, and the content of neurotransmitters in the brain were observed Effects of Renshen Baizhu Zaoren Granules on autonomous activity and sleep function of normal mice were observed Results: Compared with model group, Chinese medicine high-dose group could improve the symptoms of deficiency of heart-spleen in model mice, prolong swimming time and sleep time, decrease tail immobile time, increase 5-HT content and decrease Glu content (P < 0.05, P < 0.01). In addition, it could reduce the number of autonomous activities, prolong the sleep time and shorten the sleep latency of normal mice (P < 0.05, P < 0.01). Conclusion: Renshen Baizhu Zaoren Granules has the effects of invigorating spleen and nourishing heart, quieting the heart and tranquilizing mind, improving sleep function, and its mechanism may be related to the regulation of the contents of 5-HT and Glu in brain.