更新于：2024-11-01

Rh factor x Immunoglobulin

更新于：2024-11-01

基本信息

关联

项与 Rh factor x Immunoglobulin 相关的药物

Rh immunoglobulin-VF

人免疫球蛋白气雾剂

靶点

Immunoglobulin x Rh factor

作用机制

免疫球蛋白激动剂 [+1]

在研机构

CSL Behring LLC

原研机构

CSL Behring LLC

在研适应症

血液疾病

非在研适应症-

最高研发阶段批准上市

首次获批国家/地区

澳大利亚 [+1]

首次获批日期2015-09-21

100 项与 Rh factor x Immunoglobulin 相关的临床结果

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100 项与 Rh factor x Immunoglobulin 相关的转化医学

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0 项与 Rh factor x Immunoglobulin 相关的专利（医药）

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项与 Rh factor x Immunoglobulin 相关的文献（医药）

2006-03-27·Clinical and Experimental Immunology3区 · 医学

The analysis and quantification of a clonal B cell response in a hyperimmunized anti-D donor

3区 · 医学

Article

作者: Stott, L M ; Verhagen, O J H M ; Dohmen, S E ; Aalberse, R C ; Van Der Schoot, C E ; Urbaniak, S J ; De Groot, S M

SummaryHealthy volunteers are hyperimmunized with RhD-positive red cells in order to obtain plasma containing high titres of anti-D immunoglobulin, which is used for the prevention of haemolytic disease of the fetus and newborn. We analysed the anti-D immune response in a donor who had been hyperimmunized for 7 years and who showed declining anti-D titres despite re-immunization. A phage display library representing the complete immunorepertoire and a second library representing the IGHV3 superspecies family genes (IGHV3s) repertoire in the donor were constructed and analysed. A clonal Ig-gene rearrangement was quantified in the peripheral blood by limiting dilution polymerase chain reaction (PCR) All RhD-binding phages from both libraries, except one, had heavy chains with IGH–VDJ rearrangements of the same clonal origin, but with different patterns of somatic mutations and joined with different light chains. Limiting dilution PCR performed on mRNA and genomic DNA showed a frequency of 1 clonal B cell in 2000 IgG1/3-positive B cells. We show the presence of clonally related RhD-specific B cells in a hyperimmunized anti-D donor who had declining anti-D titres and who was unresponsive to re-immunization. Furthermore, we found a high frequency of clonal B cells. These results contribute to the understanding of the immune response against RhD in hyperimmunized anti-D donors.

2001-04-15·Blood1区 · 医学

Coexpression of band 3 mutants and Rh polypeptides: differential effects of band 3 on the expression of the Rh complex containing D polypeptide and the Rh complex containing CcEe polypeptide

1区 · 医学

Article

作者: Anstee, David J. ; Smythe, Jonathan S. ; Beckmann, Roland ; Tanner, Michael J. A.

AbstractK562 cells were stably transfected with cDNAs encoding the band 3 found in Southeast Asian ovalocytosis (B3SAO, deletion of residues 400-408), band 3 with a transport-inactivating E681Q point mutation (B3EQ), or normal band 3 (B3). Flow cytometric analysis and quantitative immunoblotting revealed that B3SAO expressed alone was translocated to the plasma membrane, at levels similar to B3 or B3EQ. Nine monoclonal antibodies that reacted with extracellular loops of B3 also reacted with B3SAO, although the affinity of most antibodies for the mutant protein was reduced. Both known Wrb epitopes were expressed on K562/B3SAO cells, demonstrating that B3SAO interacts with glycophorin A. The growth rates of K562 clones expressing equivalent amounts of B3 and B3EQ were the same, suggesting that the potentially toxic transport function of band 3 may be regulated in K562 cells. The band 3–mediated enhancement of Rh antigen reactivity and the depression of Rh epitopes on SAO erythrocytes were investigated by comparing the coexpression of B3, B3SAO, or B3EQ in K562 clones expressing exogenous RhcE or RhD polypeptides. The results are consistent with an interaction between band 3 and the Rh polypeptide–Rh glycoprotein (RhAG) complex, which may enhance translocation of the complex or affect its conformation in the plasma membrane. The data suggest that the interaction between band 3 and the RhD–RhAG complex is weaker than it is between band 3 and the RhCcEe–RhAG complex.

1995-09-01·Human Antibodies

The biological activity of human monoclonal IgG anti-D is reduced by β-galactosidase treatment

Article

作者: Rook, Graham A.W. ; Hadley, Andrew G. ; Griffiths, Helen L. ; Kumpel, Belinda M. ; Wang, Yan

The contribution to IgG effector function of exposed galactose residues on the oligosaccharide chains in IgG has been tested experimentally. We studied a human monoclonal antibody (BRAD-5) to the Rh D blood group antigen. The preparation used contained a very low percentage of agalactosyl IgG (3.6%). After digestion with beta-galactosidase there was an increase in terminal GlcNAc indicating an increase in % agalactosyl IgG to approximately 30%. Comparison was made of the Fc receptor-(Fc gamma R)-mediated functional interactions of the two glycoforms of BRAD-5 in assays which measured the recognition and destruction of sensitised erythrocytes by various effector cells. After beta-galactosidase treatment, there was a slight reduction in Fc gamma RI-mediated adherence of erythrocytes to U937 cells and phagocytosis of erythrocytes by monocytes. Fc gamma RII-mediated binding of erythrocytes to K562 cells was also reduced. However there was little difference in adherence of erythrocytes to either Daudi cells (via Fc gamma RII) or NK cells (via Fc gamma RIII). There was a consistent reduction in lysis of erythrocytes mediated through Fc gamma RIII on K cells with the beta-galactosidase treated anti-D. Overall the results showed that reduced levels of galactose on IgG anti-D were associated with reduced biological activity in these assays, but the experiments failed to cast light on the physiological role of the agalactosyl glycoform of IgG.