Background:Hematologic diseases have seriously threatened human health. Although hematopoietic
stem cell transplantation (HSCT) is an effective curative option, the complications, especially
graft-versus-host disease (GVHD), are a big problemMethods:TNF-α pretreatment of hematopoietic stem cells. Apoptosis was detected by flow cytometry,
Transwell, and wound healing assays were used to assess cell migration and invasion, E-selectin expression
was observed by fluorescence imaging, the levels of NO were measured by a kit, the expression of Ecadherin,
MMP2, and MMP9 was detected in cells by qRT-PCR, and western blot was used to analyze
the expression of E-cadherin, CXCL12, MCP-1, MCP-3, MMP2, and MMP9.Results:TNF-α induces a high apoptosis rate of CD3, CD19, and CD133 and a low apoptosis rate of
CD34. The level of Fas and TNF-R1 was significantly high than that of TNF-R2. HSCs treated with TNF-
α declined the invasion and migration of HUVECs. E-selectin, MMP2 and MMP9 mRNA levels of HUVECs
and MMP2, CXCL12, MCP-1, and MCP-3 were decreased after HSCs-TNF-α treatment, while the
E-cadherin mRNA and protein level of HUVECs was enhanced with HSCs-TNF-α treatment.Conclusion:TNF-α pretreated HSCs can lead to reduced levels of migration, adhesion, and chemokines
of HUVECs, thereby declining the inflammatory response and GVHD.