更新于：2024-11-01

ACTA1

更新于：2024-11-01

基本信息

别名

ACTA、ACTA1、actin alpha 1, skeletal muscle

+ [4]

简介

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

关联

项与 ACTA1 相关的药物

WO2023186189

专利挖掘

靶点

ACTA1

作用机制-

在研机构

扬州大学

原研机构

扬州大学

在研适应症

肿瘤

非在研适应症-

最高研发阶段药物发现

首次获批国家/地区-

首次获批日期1800-01-20

100 项与 ACTA1 相关的临床结果

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100 项与 ACTA1 相关的转化医学

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0 项与 ACTA1 相关的专利（医药）

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4,842

项与 ACTA1 相关的文献（医药）

2024-12-01·International Immunopharmacology

Empagliflozin protects the heart from atrial fibrillation in rats through inhibiting the NF-κB/HIF-1α regulatory axis and atrial remodeling

Article

作者: Badreldin, Hussein ; Ibrahim, Tarek ; Elshal, Mahmoud ; El-Karef, Amr

Atrial fibrillation (AF) is the most common form of sustained cardiac arrhythmia. The current study aimed to investigate the potential of empagliflozin (EMPA) to protect against acetylcholine (ACh)/calcium chloride (CaCl₂)-induced AF in rats and elucidate the possible underlying mechanism of action. Rats were randomly assigned to five groups, as follows: CTRL group: received 1 ml/kg isotonic saline; AF group: received 1 ml/kg induction mixture of ACh/CaCl₂ (60 µg ACh and 10 mg CaCl₂ per ml); EMPA group: received 30 mg/kg EMPA; AF + EMPA10 group: received the induction mixture concurrent with 10 mg/kg EMPA; AF + EMPA30 group: received the induction mixture concurrent with 30 mg/kg EMPA. Our results showed that EMPA administration inhibited the AF-related electrocardiographic abnormalities and decreased the serum brain natriuretic peptide levels. EMPA treatment maintained the cardiac redox balance, as indicated by reduced levels of the lipid peroxidation biomarker malonaldehyde while enhancing the antioxidant glutathione levels. Moreover, EMPA markedly repressed ACh/CaCl₂-induced C-reactive protein, tumor necrosis factor, and interleukin-6 production. Interestingly, EMPA administration strongly suppressed cardiac transforming growth factor beta1, collagen type I, and alpha-smooth muscle actin expression levels in the AF rats. These results were consistent with our histopathological findings, which revealed the ameliorative effect of EMPA on AF-induced inflammatory and fibrotic lesions. Mechanistically, EMPA dose-dependently downregulated nuclear factor-kappa B (NF-κB) and hypoxia-inducible factor (HIF)-1α expressions. Besides, it attenuated the pro-apoptotic active caspase-3 while augmenting the anti-apoptotic B-cell lymphoma 2 expressions. Furthermore, EMPA dose-dependently suppressed cardiac phosphatidylinositol 3-kinase (PI3K)/Akt signaling. In conclusion, this study demonstrates that EMPA intervention, within AF induction, protects against ACh/CaCl₂-induced AF in rats, exerting powerful antioxidant, anti-inflammatory, anti-fibrotic, and anti-apoptotic effects. These effects are mainly mediated through the targeting of the NF-κB/HIF-1α regulatory axis in a dose-dependent manner.

2024-11-01·Journal of Veterinary Diagnostic Investigation

A congenital periocular leiomyosarcoma in a dairy calf

Article

作者: García, Juan A. ; Moscuzza, Hernán C. ; García, Jorge P. ; Rivulgo, Margarita V. ; Cantatore, Sofía E. ; Rosatti, Juan J. ; Uzal, Francisco A. ; Tambella, Victoria M. ; Riccio, Belén M. ; Viviani, Paula

A mass was removed surgically from the right orbit of a 1-d-old Holstein calf. Grossly, the mass filled the rostral part of an enlarged orbit and compressed the globe toward the caudal pole of the orbit. The brown, 6-cm tumor had central yellow and brown areas, and a smooth, glistening cut surface. Microscopically, the neoplasm was highly cellular and composed of spindle cells arranged in irregular, broad, interlacing streams and bundles, forming a herringbone pattern and supported by a sparse collagenous matrix. Neoplastic cells infiltrated surrounding soft tissues and compressed the globe. The neoplastic cells had positive immunolabeling for α–smooth muscle actin, desmin, and vimentin, and negative immunolabeling for factor VIII, myoglobin, cytokeratin, and skeletal muscle actin. Histopathology and immunohistochemistry results confirmed a diagnosis of leiomyosarcoma. To our knowledge, congenital periocular leiomyosarcoma has not been reported in cattle previously. This rare tumor could be included as a differential diagnosis in newborn calves with periocular masses.

2024-11-01·Biomedicine & Pharmacotherapy

Soybean Extract Ameliorates Lung Injury induced by Uranium Inhalation: An integrated strategy of network pharmacology, metabolomics, and transcriptomics

Article

作者: Liu, Qimiao ; Su, Lixia ; Wu, Qingdong ; Pang, Wanqing ; Liang, Hongying ; Dong, Ruifeng ; Gu, Xiaona ; Xue, Xiangming ; Liu, Mengya ; Yang, Xin ; Tang, Yufu ; Zhan, Jingming

AIMThis study aimed to evaluate the protective effect of soybean extract (SE) against uranium-induced lung injury in rats.MATERIALS AND METHODSA rat lung injury model was established through nebulized inhalation of uranyl nitrate. Pretreatment with SE or sterile water (control group) by gavage for seven days before uranium exposure and until the experiment endpoints. The levels of uranium in lung tissues were detected by ICP-MS. Paraffin embedding-based hematoxylin & eosin staining and Masson's staining for the lung tissue were performed to observe the histopathological imaging features. A public database was utilized to analyze the network pharmacological association between SE and lung injury. The expression levels of proteins indicating fibrosis were measured by enzyme-linked immunosorbent assay. RNA-seq transcriptomic and LC-MS/MS targeted metabolomics were conducted in lung tissues.RESULTSUranium levels in the lung tissues were lower in SE-pretreated rats than in the uranium-treated group. Inflammatory cell infiltration and the deposition of extracellular matrix were attenuated, and the levels of alpha-smooth muscle actin, transforming growth factor beta1, and hydroxyproline decreased in SE-pretreated rats compared to the uranium-treated group. Active ingredients of SE were related to inflammation, oxidative stress, and drug metabolism. A total of 67 differentially expressed genes and 39 differential metabolites were identified in the SE-pretreated group compared to the uranium-treated group, focusing on the drug metabolism-cytochrome P450, glutathione metabolism, IL-17 signaling pathway, complement, and coagulation cascades.CONCLUSIONSThese findings suggest that SE may ameliorate uranium-induced pulmonary inflammation and fibrosis by regulating glutathione metabolism, chronic inflammation, and immune regulation.

项与 ACTA1 相关的新闻（医药）

2022-06-14

·生物谷

新发现！科学家揭示了结直肠癌成纤维细胞异质性新机制

结直肠癌是世界上第三大常见恶性肿瘤，预计到2030年，全球发病率将超过200万，死亡率将达到100万。在结直肠癌等实体瘤患者中，超过90%的死亡是由于转移。结直肠癌是世界上第三大常见恶性肿瘤，预计到2030年，全球发病率将超过200万，死亡率将达到100万。在结直肠癌等实体瘤患者中，超过90%的死亡是由于转移。这突出了研究CRC进展生物学的重要性，以确定更好的预后标志物和设计有针对性的治疗。全球公认的分类系统将结直肠癌和分层患者的共识分子亚型(CM)定义为四个亚组(CMS1-4)。其中，CMS4预后最差，与肿瘤转移和化疗耐药有关。重要的是，这个亚型在突变负担、体细胞拷贝数改变或CpG岛甲基化方面与其他亚型没有区别，而是存在间质细胞和间充质(或转化生长因子-β激活)基因表达特征。因此，在转移细胞和转移细胞之间似乎存在着重要的联系。图片来源: https://doi.org/10.1002/jev2.12226 近日，来自南安普敦大学的研究者们在J Extracell Vesicles杂志上发表了题为“Epithelial to mesenchymal transition influences fibroblast phenotype in colorectal cancer by altering miR-200 levels in extracellular vesicles”的文章，该研究为结直肠癌成纤维细胞异质性的发展提供了一种新的机制，通过EV介导的miRNAs转移，并解释了为什么转移潜能较大的结直肠癌富含CAF。具有间充质基因表达特征的结直肠癌(CRC)具有最大的远处转移倾向，其特征是与癌症相关的成纤维细胞在间质中聚集。研究者研究了结直肠癌细胞上皮向间充质转化的状态是否影响成纤维细胞的表型，重点是细胞外小泡(EV)的转移，作为细胞间通讯的受控手段。上皮性结直肠癌EVS抑制转化生长因子-β诱导的肌成纤维细胞分化，而间叶性结直肠癌EVS不抑制其分化。这是由miR-200(miR-200a/b/c，-141)驱动的，它富含上皮性CRC-EV，并转移到受体成纤维细胞。在成纤维细胞中异位表达miR-200或ZEB1基因敲除，同样抑制肌成纤维细胞的分化。研究者发现在TCGA数据集中的一组CRC患者中，miR-200和肌成纤维细胞标志物之间存在强烈的负相关。在体内实验中，研究者通过将上皮或间充质CRC细胞与成纤维细胞共同注射，并分析肌成纤维细胞表型的基质标记。与间叶性肿瘤相比，上皮性肿瘤的成纤维细胞表达更多的miR-200，表达更少的ACTA和FN。 CRC EMT在体内测定成纤维细胞表型中的作用图片来源: https://doi.org/10.1002/jev2.12226 总体而言，本研究揭示了CMS4肿瘤中肌成纤维细胞间质积聚的机制，主要是成纤维细胞中的miR-200/ZEB1轴，以及肿瘤细胞来源的EVS对其调节。CRC的EMT状态决定成纤维细胞表型的证明是理解不同成纤维细胞表型如何在肿瘤中存在的关键环节。本研究为最近发表的实体癌间质异质性的观察提供了机制上的见解。更好地理解这一概念将使预后基质生物标志物的开发和调节CAF表型的能力获得治疗优势。（生物谷 Bioon.com）参考文献 Rahul Bhome et al. Epithelial to mesenchymal transition influences fibroblast phenotype in colorectal cancer by altering miR-200 levels in extracellular vesicles. J Extracell Vesicles. 2022 May;11(5):e12226. doi: 10.1002/jev2.12226.