作者: Araújo, Fabiana ; da Cunha, Karina Patrícia Vieira ; Pinheiro, Thaís Lopes ; Capelo-Neto, José ; Monicelli, Fernanda ; Becker, Vanessa ; Frota, André

Cyanobacterial blooms have been a growing problem in water bodies and attracted attention from researcher and water companies worldwide. Different treatment methods have been researched and applied either inside water treatment plants or directly into reservoirs. We tested a combination of coagulants, polyaluminium chloride (PAC) and iron(III) chloride (FeCl₃), and ballasts, luvisol (LUV) and planosol (PLAN), known as the 'Floc and Sink' technique, to remove positively buoyant cyanobacteria from a tropical reservoir water. Response Surface Methodology (RSM) based on Central Composite Design (CCD) was used to optimize the two reaction variables - coagulant dosage (x₁) and ballast dosage (x₂) to remove the response variables: chlorophyll-a, turbidity, true color, and organic matter. Results showed that the combination of LUV with PAC effectively reduced the concentration of the response variables, while PLAN was ineffective in removing cyanobacteria when combined to PAC or FeCl₃. Furthermore, FeCl₃ presented poorer floc formation and lower removal efficiency compared to PAC. This study may contribute to the theoretical and practical knowledge of the algal biomass removal for mitigating eutrophication trough different dosages of coagulants and ballasts.

2023-01-02·Journal of Immunoassay and Immunochemistry

A simple and high -performance immobilization technique of membrane protein from crude cell lysate sample for a membrane-based immunoassay application

Article

作者: Khemthongcharoen, Numfon ; Jeamsaksiri, Wutthinan ; Yookong, Nutthapon ; Juntasaro, Ekachai ; Uawithya, Panapat ; Sripumkhai, Witsaroot ; Pattamang, Pattaraluck ; Kamnerdsook, Ampol ; Chanasakulniyom, Mayuree ; Promptmas, Chamras ; Houngkamhang, Nongluck

Membrane proteins are difficult to be extracted and to be coated on the substrate of the immunoassay reaction chamber because of their hydrophobicity. Traditional method to prepare membrane protein sample requires many steps of protein extraction and purification that may lead to protein structure deformation and protein dysfunction. This work proposes a simple technique to prepare and immobilize the membrane protein suspended in an unprocessed crude cell lysate sample. Membrane fractions in crude cell lysate were incorporated with the large unilamellar vesicle (LUV) that was mainly composed of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) before coating in the polystyrene plate by passive adsorption technique. Immunofluorescence staining and the Enzyme-Linked Immunosorbent Assay (ELISA) examination of a strictly conformation-dependent integral membrane protein, Myelin Oligodendrocyte Glycoprotein (MOG), demonstrate that LUV incorporated cell lysate sample obviously promotes MOG protein immobilization in the microplate well. With LUV incorporation, the dose-response curve of the MOG transfected cell lysate coating plate can be 2-9 times differentiated from that of the untransfected cell lysate coating plate. The LUV incorporated MOG transfected cell lysate can be efficiently coated in the microplate without carbonate/bicarbonate coating buffer assistance.

2021-05-01·Plant Disease2区 · 农林科学

Variability of Phytoplasma Infection Density in Poinsettia and Evaluation of its Association with the Level of Branching in Host Plants

2区 · 农林科学

Article

作者: Chu, Chia-Ching ; Lee, Shin ; Chu, Chien-Young

It has long been established that phytoplasma infection is the cause of the free-branching phenotype in poinsettia. However, relatively little is known about the ecology of the pathogen in planta. The present study evaluated the infection pattern of poinsettia branch-inducing phytoplasma (PoiBI) and its association with the poinsettia phenotype during cutting propagation. The presence of this pathogen in the poinsettia variety Luv U Pink was determined using PCR and sequence analysis. The infection density of PoiBI in distinct tissue types of different plant segments were then determined using quantitative PCR coupled with plasmid-based standard curves. Both vegetative-stage and flowering-stage plants were tested. The results showed that, despite being considerably variable among plants, the infection densities of PoiBI tend to be higher in source leaves located in the lower parts of the plant. The densities were consistently lower in tissues located at the top of the plants, regardless of the tissue type. Analysis of the infection densities among samples collected from six stock plants used in commercial production also revealed significantly different levels of PoiBI load. An association between PoiBI infection density in the stock plants and the level of branching in cutting-propagated plants (derived from the stock plants) was also observed; stock plants with low infection densities tended to produce smaller proportions of plants exhibiting higher degrees of branching both before and after pinching. These data suggest that uneven distribution of PoiBI within and among stock plants may lead to the production of cuttings with variable phytoplasma densities which may, in turn, affect the phenotypic uniformity of the plants produced. Overall, findings from the present work add to the understanding of PoiBI’s ecology and could provide implications to commercial poinsettia production.

100 项与 ETC-588 相关的药物交易

登录后查看更多信息

外链

KEGG	Wiki	ATC	Drug Bank
-	-	-	DB05456

研发状态

10 条进展最快的记录,

后查看更多信息

适应症	最高研发状态	国家/地区	公司	日期
急性冠状动脉综合征	临床2期	-	Pfizer Inc.	-
急性冠状动脉综合征	临床2期	美国	Esperion Therapeutics, Inc.	-
急性冠状动脉综合征	临床2期	加拿大	-	-
动脉粥样硬化	临床2期	美国	Esperion Therapeutics, Inc.	-
动脉粥样硬化	临床2期	-	Pfizer Inc.	-
动脉粥样硬化	临床2期	加拿大	-	-