Thymol

At present, the treatment of diabetic wounds is challenging and time-consuming. In this study, a delivery system combining thymol-loaded Pickering emulsion and gel film has been investigated to accelerate wound healing. The nanoparticles (SPI-CMC) composed of soy protein isolate (SPI) and carboxymethyl cellulose (CMC) were used to stabilize Pickering emulsion and loaded into polyvinyl alcohol (PVA)-porous starch (PS)-hyaluronic acid (HA) gel membrane (PPH) matrix. The PPH matrix was loaded with thymol (PPH+T), which effectively solves the problem of solubility and compatibility of thymol. Moreover, the PPH+T matrix based on Pickering emulsion and gel membrane achieving the antibacterial, anti-inflammatory, angiogenesis and tissue collagen formation effect. The composite gel membrane exhibited a long-term sustained release of thymol for up to 48 h, and had good antibacterial activity against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus). The antibacterial diameters of the two were 19.73 ± 1.375 mm and 21.94 ± 0.486 mm, respectively. It was verified in animal experiments that the healing of normal wounds and diabetic wounds was accelerated, and the final healing rates were 97 % and 94 %, respectively. Therefore, the composite gel membrane prepared in this study has great potential in wound antibacterial and healing, and provides a basis for effective treatment of chronic wounds.

The purpose of this research was to assess the effectiveness of a combination of monoterpenes (thymol and carvacrol) in treating juvenile Nile tilapia ( Oreochromis niloticus ) infected with Edwardsiella tarda as a potential substitute for the antibiotic oxytetracycline. The study utilised different concentrations of the monoterpenes blend (MTs): 0.4, 0.6 and 0.8 g kg −1 of feed. A negative control group received a diet without the additive, while a positive control group was given medicated feed containing oxytetracycline (OTC) (11.85 g kg −1 ) for a 10‐day treatment period. Cumulative mortality (%), haematological and immunological parameters were assessed after the feed administration. The negative control group experienced a mortality rate of 53%, while fish treated with medicated feed had a significantly lower mortality rate of 3%. The addition of MTs at concentrations of 0.4 g kg −1 (13% mortality), 0.6 g kg −1 (8% mortality) and 0.8 g kg −1 (0% mortality) also resulted in reduced mortality rates, with the highest concentration showing survival rates comparable to the oxytetracycline group. Following the 10‐day treatment period, no mortality was observed in the treated groups, indicating the efficacy of MTs‐supplemented feed in alleviating the clinical symptoms of Edwardsiellosis. The haematological parameters indicated that as the dosage of MTs increased, the mean corpuscular volume (MCV) decreased. The MCV was significantly lower in the 0.6 and 0.8 g kg −1 treatments compared to the control. Mean corpuscular haemoglobin initially increased with the 0.4 g kg −1 MTs treatment but then decreased with higher doses. The mean corpuscular haemoglobin concentration was higher in the treated groups compared to the control. Red blood cell levels were higher in the 0.8 g kg −1 MTs treatment and lower in the 0.4 g kg −1 treatment. Ht was higher in the control group and lower in the 0.4 g kg −1 MTs treatment. Haemoglobin levels followed a similar trend, with higher values in the control group. The 0.8 g kg −1 MTs treatment had the highest number of total leucocytes and lymphocytes. The control group had the highest number of neutrophils, while the 0.8 g kg −1 MTs group had higher numbers of monocytes, eosinophils, basophils and thrombocytes. The negative control exhibited the lowest antimicrobial titre compared to the OTC and the various concentrations of MTs. The concentrations of MTs (0.4, 0.6 and 0.8 g kg −1 ) demonstrated similar average values and were more effective than the control and OTC. There was no significant difference in agglutinating titre among MTs, control and OTC. Thymol and carvacrol notably decreased the mortality of tilapia infected with E. tarda , with a dosage as high as 0.8 g kg −1 showing equivalent effectiveness to oxytetracycline. Furthermore, they enhanced haematological and immunological parameters, indicating that MTs could be a promising alternative to antibiotic use.