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KANAZAWA, Japan, Aug. 26, 2024 /PRNewswire/ -- Researchers at the Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, used high-speed atomic force microscopy to observe dynamic changes in AMPA receptors, which are vital for brain communication. Their findings, published in ACS Nano, reveal how these receptors adapt during signal transmission and suggest potential targets for neurological therapies. This study, led by Mikihiro Shibata, delves into the complex behavior of AMPA receptors (AMPARs), which are vital for communication between nerve cells in the brain. AMPARs are responsible for fast excitatory neurotransmission, a process crucial for learning, memory, and overall cognitive function. The research particularly focuses on the GluA2 subunit of AMPARs, a key component in transmitting signals at synapses, the junctions where neurons connect. The team employed an advanced imaging technique known as high-speed atomic force microscopy (HS-AFM) to observe the real-time behavior of the N-terminal domain (NTD) in the GluA2 subunit. The NTD is the starting segment of the protein, playing a critical role in how AMPARs function and cluster at synapses. The study also examined how the GluA2 subunit interacts with TARP γ2, a regulatory protein that fine-tunes the receptor's response to signals. One of the key findings is the behavior of the NTD in different states: resting, activated, and desensitized. The researchers discovered that in the activated state, the NTD dimers—pairs of NTDs—can split into single units or monomers. This process, known as subunit exchange, allows parts of one receptor to s another, potentially altering the receptor's function. This novel observation was supported by molecular dynamics simulations, which showed that these monomeric states are stable in their lipid environment, providing a potential mechanism for receptor adaptability and diversity. In the desensitized state, where the receptor becomes less responsive to signals, the NTD dimers separate, but their movement is more restricted compared to the activated state. This desensitization helps protect nerve cells from overstimulation, which could lead to cellular damage. The study's insights into the structural changes of the NTDs in different functional states highlight the dynamic nature of AMPARs and their ability to adapt to various conditions within the synaptic environment. The research also sheds light on the role of neuronal pentraxin 1 (NP1), a protein that aids in the clustering of AMPARs at synapses. NP1 forms a ring-shaped structure that binds to the tips of the NTDs, potentially facilitating the gathering of multiple AMPARs into clusters. This clustering is essential for efficient synaptic transmission, as it brings receptors closer together, allowing for more effective signaling between neurons. By linking multiple receptors, NP1 enhances the strength and reliability of the synaptic connection, contributing to the overall efficiency of neural communication. The study's findings contribute significantly to our understanding of how AMPARs function and adapt during neurotransmission. By revealing the dynamic structural changes in the NTDs and highlighting the role of NP1 in receptor clustering, the research offers new insights into the molecular processes that underlie synaptic plasticity—the ability of synapses to strengthen or weaken over time, which is essential for learning and memory. These discoveries could have important implications for developing treatments for neurological disorders where AMPAR function is disrupted, such as in epilepsy, Alzheimer's disease, and other cognitive impairments. As the authors conclude, "Our research reveals the dynamic structural changes that occur within AMPA receptors, underscoring their remarkable adaptability. Understanding these mechanisms not only deepens our knowledge of brain function but also opens new avenues for therapeutic interventions targeting synaptic transmission and plasticity." Glossary AMPA Receptors (AMPARs) Proteins on the surface of nerve cells essential for communication between cells in the brain. They respond to signals by allowing charged particles to enter the cell, triggering various processes like learning and memory formation. N-terminal domain (NTD) The beginning part of a protein that influences how the protein behaves and interacts with other molecules. For AMPARs, the NTD helps them cluster together at nerve cell connections. Synapse The point where two nerve cells connect and communicate, allowing signals to pass from one cell to another. TARP γ2 A regulatory protein that interacts with AMPARs to control their response to signals. High-speed atomic force microscopy (HS-AFM) This imaging technique uses a nanosized tip at the end of a cantilever that is scanned over a sample. It can be used to determine the topography of a sample surface from the change in the strength of forces between the tip and the sample with distance, and the resulting deflection of the cantilever. It was first developed in the 1980s but a number of modifications have augmented the functionality of the technique since. It is better suited to imaging biological samples than the scanning tunnelling microscope developed that had been developed because it does not require a conducting sample. In the 2000s Toshio Ando at Kanazawa University was able to improve the scanning speed to such an extent that moving images could be captured. This allowed people to use the technique to visualize molecular processes for the first time. Desensitized state A condition where the receptor becomes less responsive to signals, often to prevent overstimulation. Neuronal pentraxin 1 (NP1) A protein that clusters AMPARs at synapses, improving their signaling ability. Subunit exchange The process where parts of a protein (subunits) s receptors, potentially altering their function. Related figure and caption High-speed AFM directly visualized the real-space and real-time structural dynamics of AMPARs in the presence of ligands in a liquid environment. © 2024 Sumino, et al., ACS Nano Reference Ayumi Sumino, Takashi Sumikama, Yimeng Zhao, Holger Flechsig, Kenichi Umeda, Noriyuki Kodera, Hiroki Konno, Motoyuki Hattori, and Mikihiro Shibata, High-speed atomic force microscopy reveals dynamic dimer splitting of the N-terminal domain of GluA2 ionotropic glutamate receptor-auxiliary subunit complex, Published online 23 August 2024, ACS Nano 2024. DOI: 10.1021/acsnano.4c06295 URL: Funding acknowledgements This research was supported by the World Premier International Research Center Initiative (WPI), MEXT, Japan (to M.S.), JSPS KAKENHI grant numbers JP20K21122 (to M.S.) and JP21H01771 (to M.S.), JSPS Bilateral Program Number JP120247404 (to M.S.), the Mochida Memorial Foundation for Medical and Pharmaceutical Research (to M.S.), the Uehara Memorial Foundation (to M.S.), the National Natural Science Foundation of China (32071234, 32271244, 32250610205, and 32411540020 to M.H.), an innovative research team of high-level local universities in Shanghai and a key laboratory program of the Education Commission of Shanghai Municipality (ZDSYS14005 to M.H.), and JST/CREST (JPMJCR1762 to N.K. and H.F.). Contact Kimie Nishimura (Ms) Project Planning and Outreach NanoLSI Administration Office, Nano Life Science Institute (WPI-NanoLSI) Kanazawa University Kakuma-machi, Kanazawa 920-1192, Japan Email: [email protected] Tel: +81 (76) 234-4555 About Nano Life Science Institute (WPI-NanoLSI), Kanazawa University Understanding nanoscale mechanisms of life phenomena by exploring "uncharted nano-realms". Cells are the basic units of almost all life forms. We are developing nanoprobe technologies that allow direct imaging, analysis, and manipulation of the behavior and dynamics of important macromolecules in living organisms, such as proteins and nucleic acids, at the surface and interior of cells. We aim at acquiring a fundamental understanding of the various life phenomena at the nanoscale. About the World Premier International Research Center Initiative (WPI) The WPI program was launched in 2007 by Japan's Ministry of Education, Culture, Sports, Science and Technology (MEXT) to foster globally visible research centers boasting the highest standards and outstanding research environments. Numbering more than a dozen and operating at institutions throughout the country, these centers are given a high degree of autonomy, allowing them to engage in innovative modes of management and research. The program is administered by the Japan Society for the Promotion of Science (JSPS). See the latest research news from the centers at the WPI News Portal: Main WPI program site: About Kanazawa University As the leading comprehensive university on the Sea of Japan coast, Kanazawa University has contributed greatly to higher education and academic research in Japan since it was founded in 1949. The University has three colleges and 17 schools offering courses in subjects that include medicine, computer engineering, and humanities. The University is located on the coast of the Sea of Japan in Kanazawa – a city rich in history and culture. The city of Kanazawa has a highly respected intellectual profile since the time of the fiefdom (1598-1867). Kanazawa University is divided into two main campuses: Kakuma and Takaramachi for its approximately 10,200 students including 600 from overseas. SOURCE Kanazawa University