An Exploratory SPECT Imaging Study to Assess the Utility of High-specific Activity 99mTc-HMPAO Labeling as a Tool to Detect PBMC and Lymphocyte Trafficking in the Small Bowel or Ileo-caecal Region of Crohn's Disease Patients

Using scintigraphic imaging including planar scintigraphy and SPECT, this study will evaluate the utility of two different ex vivo 99mTc-HMPAO labelled mononuclear cell populations in order to select the optimal methodology (using PBMC or purified lymphocyte subpopulations) for future drug intervention studies in Crohn's disease.
Two parallel exploratory approaches will be investigated to enrich for lymphocyte populations expressing leukocyte trafficking inhibitors. In the first, whole blood will be fractionated on a ficoll gradient to purify a heterogeneous population of all the peripheral blood mononuclear cells (PBMC) for labelling. Secondly, further enrichment will be attempted using depletion of PBMC fractions of monocytes and B cells.

开始日期2010-01-01

申办/合作机构

GSK Plc

100 项与锝[99mTc]依沙美肟相关的临床结果

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100 项与锝[99mTc]依沙美肟相关的转化医学

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100 项与锝[99mTc]依沙美肟相关的专利（医药）

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255

项与锝[99mTc]依沙美肟相关的文献（医药）

2024-03-01·Journal of Nuclear Medicine

Triggered Seizures for Ictal SPECT Imaging: A Case Series and Feasibility Study

Article

作者: Wiest, Roland ; Barlatey, Sabry L ; Friedrichs-Maeder, Cecilia ; Nowacki, Andreas ; Baud, Maxime O ; Schindler, Kaspar ; Mignardot, Camille G ; Rominger, Axel ; Haenggi, Matthias ; Pollo, Claudio ; Z'Graggen, Werner J ; Pyka, Thomas

Ictal SPECT is an informative seizure imaging technique to tailor epilepsy surgery. However, capturing the onset of unpredictable seizures is a medical and logistic challenge. Here, we sought to image planned seizures triggered by direct stimulation of epileptic networks via stereotactic electroencephalography (sEEG) electrodes. Methods: In this case series of 3 adult participants with left temporal epilepsy, we identified and stimulated sEEG contacts able to trigger patient-typical seizures. We administered ^99mTc-HMPAO within 12 s of ictal onset and acquired SPECT images within 40 min without any adverse events. Results: Ictal hyperperfusion maps partially overlapped concomitant sEEG seizure activity. In both participants known for periictal aphasia, SPECT imaging revealed hyperperfusion in the speech cortex lacking sEEG coverage. Conclusion: Triggering of seizures for ictal SPECT complements discrete sEEG sampling with spatially complete images of early seizure propagation. This readily implementable method revives interest in seizure imaging to guide resective epilepsy surgery.

2021-11-01·Clinical Nuclear Medicine3区 · 医学

No Evidence to Favor 99mTc-HMPAO or 99mTc-ECD for Ictal Brain Perfusion SPECT for Identification of the Seizure Onset Zone

3区 · 医学

Article

作者: Stodieck, Stefan ; Apostolova, Ivayla ; Lanz, Michael ; Jaber, Mariam ; Sauvigny, Thomas ; Voges, Berthold ; Lindenau, Matthias ; Martens, Tobias ; Klutmann, Susanne ; House, Patrick M ; Buchert, Ralph ; Taherpour, Jila

PurposeIctal brain perfusion SPECT with the tracer 99mTc-HMPAO or 99mTc-ECD is widely used for identification of the epileptic seizure onset zone (SOZ) in presurgical evaluation if standard pointers are uncertain or inconsistent. For both tracers, there are theoretical arguments to favor it over the other for this task. The aim of this study was to compare the performance of ictal brain perfusion SPECT between 99mTc-HMPAO and 99mTc-ECD in a rather large patient sample.Patients and MethodsThe study retrospectively included 196 patients from clinical routine in whom ictal perfusion SPECT had been performed with stabilized 99mTc-HMPAO (n = 110) or 99mTc-ECD (n = 86). Lateralization and localization of the SOZ were obtained by the consensus of 2 independent readers based on visual inspection of the SPECT images.ResultsThe 99mTc-HMPAO group and the 99mTc-ECD group were well matched with respect to age, sex, age at first seizure, duration of disease, seizure frequency, history of previous brain surgery, and findings of presurgical MRI. The proportion of lateralizing ictal SPECT did not differ significantly between 99mTc-HMPAO and 99mTc-ECD (65.5% vs 72.1%, P = 0.36). Sensitivity of ictal perfusion SPECT (independent of the tracer) for correct localization of the SOZ in 62 patients with temporal lobe epilepsy and at least worthwhile improvement (Engel scale ≤ III) 12 months after temporal epilepsy surgery was 63%.ConclusionsThis study does not provide evidence to favor 99mTc-HMPAO or 99mTc-ECD for identification of the SOZ by ictal perfusion SPECT.

2019-11-01·Journal of Applied Physiology3区 · 医学

Pharmacokinetics of ^99mTc-HMPAO in isolated perfused rat lungs

3区 · 医学

Article

作者: Barry, Katherine ; Jacobs, Elizabeth R. ; Rizzo, Benjamin M. ; Clough, Anne V. ; Audi, Said H.

Lung uptake of technetium-labeled hexamethylpropyleneamine oxime (HMPAO) increases in rat models of human acute lung injury, consistent with increases in lung tissue glutathione (GSH). Since 99mTc-HMPAO uptake is the net result of multiple cellular and vascular processes, the objective was to develop an approach to investigate the pharmacokinetics of 99mTc-HMPAO uptake in isolated perfused rat lungs. Lungs of anesthetized rats were excised and connected to a ventilation-perfusion system. 99mTc-HMPAO (56 MBq) was injected into the pulmonary arterial cannula, a time sequence of images was acquired, and lung time-activity curves were constructed. Imaging was repeated with a range of pump flows and perfusate albumin concentrations and before and after depletion of GSH with diethyl maleate (DEM). A pharmacokinetic model of 99mTc-HMPAO pulmonary disposition was developed and used for quantitative interpretation of the time-activity curves. Experimental results reveal that 99mTc-HMPAO lung uptake, defined as the steady-state value of the 99mTc-HMPAO lung time-activity curve, was inversely related to pump flow. Also, 99mTc-HMPAO lung uptake decreased by ~65% after addition of DEM to the perfusate. Increased perfusate albumin concentration also resulted in decreased 99mTc-HMPAO lung uptake. Model simulations under in vivo flow conditions indicate that lung tissue GSH is the dominant factor in 99mTc-HMPAO retention in lung tissue. The approach allows for evaluation of the dominant factors that determine imaging biomarker uptake, separation of the contributions of pulmonary versus systemic processes, and application of this knowledge to in vivo studies. NEW & NOTEWORTHY We developed an approach for studying the pharmacokinetics of technetium-labeled hexamethylpropyleneamine oxime (99mTc-HMPAO) in isolated perfused lungs. A distributed-in-space-and-time computational model was fit to data and used to investigate questions that cannot readily be addressed in vivo. Experimental and modeling results indicate that tissue GSH is the dominant factor in 99mTc-HMPAO retention in lung tissue. This modeling approach can be readily extended to investigate the lung pharmacokinetics of other biomarkers and models of lung injury and treatment thereof.

100 项与锝[99mTc]依沙美肟相关的药物交易

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KEGG	Wiki	ATC	Drug Bank
-	锝[99mTc]依沙美肟	V09AA01	DB09163

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适应症	国家/地区	公司	日期
炎症性肠病	美国	GE Healthcare, Inc.	2013-08-05
腹腔感染	美国	GE Healthcare, Inc.	2013-08-05
脑卒中	美国	GE Healthcare, Inc.	2013-08-05
脑血管疾病	日本	Nihon Medi-Physics Co., Ltd.	1989-03-31

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适应症	最高研发状态	国家/地区	公司	日期
克罗恩病	临床前	荷兰	GSK Plc	2010-01-01
克罗恩病	临床前	比利时	GSK Plc	2010-01-01

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研究	分期	人群特征	评价人数	分组	结果	评价	发布日期


EANM2018	N/A	-	-	99mTc- HMPAO (Plasma group)	範鬱簾範襯蓋鏇鹹鏇淵(鬱齋襯淵艱襯夢築獵膚) = 觸蓋願糧遞獵蓋襯蓋鑰獵簾襯夢鬱顧鏇選築築 (窪觸廠範蓋願顧鹹鹽構, 3.82) 更多	积极	2018-09-18
EANM2018	N/A	-	-	99mTc- HMPAO (Saline group)	範鬱簾範襯蓋鏇鹹鏇淵(鬱齋襯淵艱襯夢築獵膚) = 醖醖觸範範築遞蓋醖齋獵簾襯夢鬱顧鏇選築築 (窪觸廠範蓋願顧鹹鹽構, 3.48) 更多	积极	2018-09-18
SNMMI2018	N/A	感染	-	99mTc-HMPAOO-labeled leukocyte (99mTc-HMPAO labeled leukocytes)	(顧襯製襯鬱淵襯壓齋網) = 選鬱鏇淵膚淵憲蓋壓簾餘憲夢網艱範鏇積壓醖 (窪範醖鏇積鹽醖鹽壓糧 ) 更多	-	2018-05-23