Objective To compare the efficiencies of filtration of bulk of live attenuated varicella vaccine by cloth filter bag and filter column. Methods The bulk of live attenuated varicella vaccine was filtrated by cloth filter bag and filter column resp., and observed by microscopy, of which the clarities and virus titers were compared. The bulks filtered by two methods were filled, and the prepared final product of vaccine was tested for heat stability at 37°C, sterility, virus titer, residual bovine serum albumin and residual antibiotics according to the standard for license of live attenuated varicella vaccine and the requirements in Chinese Pharmacopoeia (Volume III, 2010 edition). Results No intact cells were observed in the bulks filtered by two methods, while the virus titers were basically identical. However, the clarity of bulk filtered with filter column was significantly superior to that by cloth filter bag. All the indexes of final products prepared with bulks filtered by two methods showed no significant difference, which met the standard for license of live attenuated varicella vaccine. Conclusion The efficiency of filtration by filter column during production of live attenuated varicella vaccine was significantly superior to that by cloth filter bag.