Topical application of Glyteer (5% ointment) (GL) inhibited homologous passive cutaneous anaphylaxis (PCA) in rats with the same potency as that of betamethasone 17-valerate (BV) (0.12% ointment). GL also inhibited the degranulation of mast cells induced by PCA. GL did not exert an inhibitory effect on the reversed cutaneous anaphylaxiss in rats, bufexamac (BM), indomethacin (ID), and BV did. The direct passive Arthus reaction was markedly inhibited with BV, but not with GL, BM, and ID. GL (0.2, 1, and 5%) showed a concentration-dependent inhibition on the delayed-type hypersensitivity response induced by oxazolone in mice, the activity was stronger than those of ID and BM.