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De novo RNA-sequencing of Wolfiporia cocos mycelia cultured with filter paper composed of cellulose as the sole carbon source revealed a total of five expressed β-glucosidase genes. Among these, the β-glucosidase named Wcbg1B-1, which is composed of 539 amino acid residues and belongs to the GH1 family, had the highest mRNA abundance, accounting for 65 % of the total mRNA of the five expressed β-glucosidases. The recombinant Wcbg1B-1 was successfully expressed in Escherichia coli, with an optimal pH of 6.0 and an optimal temperature of 40°C using p-nitrophenyl-β-d-glucopyranoside (pNPG) as the substrate. Recombinant Wcbg1B-1 has a K_m value of 0.32 mmol/L, a V_max of 416 μmol/min/mg and specific activity of 461.5 U/mg. Recombinant Wcbg1B-1 has strict β-glycosidic bond specificity, the highest hydrolysis activity to cellobiose, followed by lactose, and the lowest hydrolysis activity to gentian. Recombinant Wcbg1B-1 exhibits hydrolytic activity towards macromolecular cellulose such as sodium carboxymethyl cellulose, microcrystalline cellulose and filter paper. Additionally, its high tolerance to high concentrations of glucose and salt makes it more practical for cellulose hydrolysis. Recombinant Wcbg1B-1 can hydrolyze lactose at low temperatures, indicating that it could also be a potential biocatalyst for lactose-reduced dairy industry.

Four previously undescribed steroids, named sarmutogenin diginoside, sarmutogenin oleandroside, 16-hydroxy-17βH sarmutogenin digitaloside and 12-dehydroxy sarmutogenin diginoside (1-4), along with eight known ones (5-12) were isolated from the stems and leaves of Strophanthus divaricatus. The chemical structures of the new compounds including their absolute configurations, were characterized by extensive spectroscopic methods, electronic circular dichroism (ECD), X-ray diffraction, as well as comparison with literature data. Most of the isolated compounds demonstrated superior antiproliferative activities against A549 non-small cell lung cancer (NSCLC) cells compared to cisplatin, a first-line treatment regimen for NSCLC. Among these, compounds 2 and 12 can potently inhibited the growth of a panel of NSCLC cells by inducing apoptosis and G2/M cell cycle arrest.

Mucosal antigen-specific T cells are pivotal for pathogen clearance and immune modulation in respiratory infections. Dysregulated T cell responses exacerbate coronavirus disease 2019 severity, marked by cytokine storms and respiratory failure. Despite extensive description in peripheral blood, the characteristics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cells in the lungs remain elusive. Here we conducted integrated single-cell profiling of SARS-CoV-2-specific T cells in 122 bronchoalveolar lavage fluid (BALF) and 280 blood samples from 159 patients, including 27 paired BALF and blood samples from 24 patients. SARS-CoV-2-specific T cells were robustly elicited in BALF irrespective of prior vaccination, correlating with diminished viral loads, lessened systemic inflammation and improved respiratory function. SARS-CoV-2-specific T cells in BALF exhibited profound activation, along with proliferative and multi-cytokine-producing capabilities and a glycolysis-driven metabolic signature, which were distinct from those observed in peripheral blood mononuclear cells. After viral clearance, these specific T cells maintained a polyfunctional tissue-resident memory phenotype, highlighting their critical roles in infection control and long-term protection.