Bacterial toxins, called superantigens, are produced by Staphylococcus aureus and are known to activate γδ T cells. γδ T cells contribute to long-lasting immunity against bacterial skin infections caused by S. aureus. γδ T cells are a distinct subgroup of T cells containing the T cell receptor (TCR) γ and δ chains. The γδ TCR harbouring the variable chains TRGV9/TRDV2 is the most common TCR in human peripheral blood and is known to be activated by superantigens and are also promising candidates for tumor immunotherapy. However, detailed analyses of antigen binding to γδ TCR have been severely hampered by difficulties in producing large amounts of γδ TCR. In this study, we report a protocol to produce recombinant γδ TCR (TRGV9/TRDV2) by fusing the variable domains γδ TCR with the constant domains of αβ TCR. Subsequently, binding analyses were executed applying microscale thermophoresis showing a clear binding between superantigen and the γδ TCR in the micro molar range. In addition, the superantigen SEA was shown to induce cytokine expression in γδ T cells with moderate MHC dependence, suggesting that other receptors can act as antigen presenting molecules upon γδ T cell activation. These results pave the way towards a better understanding of superantigen recognition by the γδ T cells and facilitates the future use of γδ TCR in cellular tumor immunotherapy.

2025-04-01·European Journal of Immunology

Pamidronate‐Induced Clinical Remission in Chronic Non‐bacterial Osteomyelitis Is Associated with Reduced Vγ9Vδ2 T‐Cell Receptor Expression

Article

作者: Jones, Gareth W. ; Watson, Lily ; Chew, Christine ; Oliver, Elizabeth ; V Ramanan, Athimalaipet ; Segers, Francisca ; Goenka, Anu

In children with chronic non-bacterial osteomyelitis, clinical and transcriptional changes in peripheral blood were examined after pamidronate treatment. Clinically effective treatment with pamidronate was associated with reduced expression of two genes (TRDV2 and TRGV9) that encode the subunits of the Vγ9Vδ2 T-cell receptor.

1989-01-01·Nucleic Acids Research2区 · 生物学

First genomic sequence of the human T-cell receptor δ2 gene (TRDV2)

2区 · 生物学

ArticleOA

作者: Piona Dariavach ; Marie-Paule Lefranc

The genomic sequence of the human Vδ2 (TRDV2) gene is reported.One clone, λLY67Vδ2, was isolated from the LY67 library by screening with a 250-bp HindIII-PvuI fragment containing the 5' region of the cDNA X13 clone and shown to contain the TRDV2 gene.Comparison of the sequence of the genomic TRDV2 gene with those of 4 V2 cDNAs confirms that, in the human TRD locus, as in TRG, there is no somatic mutation and the variability of the delta chains depends on the combinatorial diversity of the V, D, and J gene segments and on the presence of N regions.

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