更新于：2024-11-01

amylase x S1: Chymotrypsin x Trypsin

更新于：2024-11-01

基本信息

关联

项与 amylase x S1: Chymotrypsin x Trypsin 相关的药物

POP-1

靶点

Trypsin x amylase

作用机制

S1: Chymotrypsin stimulants [+2]

在研机构

Propanc Biopharma, Inc. [+1]

原研机构

Propanc Biopharma, Inc.

在研适应症

实体瘤 [+3]

非在研适应症-

最高研发阶段临床前

首次获批国家/地区-

首次获批日期1800-01-20

JBP-1

靶点

Trypsin x amylase

作用机制

S1: Chymotrypsin stimulants [+2]

在研机构-

原研机构

Propanc Biopharma, Inc.

在研适应症-

非在研适应症

肿瘤

最高研发阶段终止

首次获批国家/地区-

首次获批日期1800-01-20

100 项与 amylase x S1: Chymotrypsin x Trypsin 相关的临床结果

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100 项与 amylase x S1: Chymotrypsin x Trypsin 相关的转化医学

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0 项与 amylase x S1: Chymotrypsin x Trypsin 相关的专利（医药）

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项与 amylase x S1: Chymotrypsin x Trypsin 相关的文献（医药）

2024-06-01·Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology

Understanding the toxicity mechanism of gelsemine in zebrafish

Article

作者: Cai, Xiaoxu ; Zhao, Baoquan ; Ma, Chenglong ; Wang, Xueyan ; Qi, Chelimuge ; Dong, Wu ; Feng, Yuanzhou ; He, Yanan ; Chang, Xu ; Bai, Meirong ; Wang, Huan

Gelsemium elegans (GE), also known as Duanchangcao, is a plant associated with toxic symptoms related to the abdomen; however, the toxicity caused by GE remains unknown. Gelsemine (GEL) is an alkaloid extracted from GE and is one of the most toxic alkaloids. This study used zebrafish as an animal model and employed high-throughput gene sequencing to identify genes and signaling pathways related to GEL toxicity. Exposure to GEL negatively impacted heart rate, swim bladder development, and activity in zebrafish larvae. Transcriptomics data revealed the enrichment of inflammatory and phagocyte signaling pathways. RT-PCR analysis revealed a decrease in the expression of pancreas-related genes, including the pancreatic coagulation protease (Ctr) family, such as Ctrl, Ctrb 1, and Ctrc, due to GEL exposure. Furthermore, GEL exposure significantly reduced Ctrb1 protein expression while elevating trypsin and serum amylase activities in zebrafish larvae. GEL also resulted in a decrease in pancreas-associated fluorescence area and an increase in neutrophil-related fluorescence area in transgenic zebrafish. This study revealed that GEL toxicity in zebrafish larvae is related to acute pancreatic inflammation.

2021-03-01·Pancreatology3区 · 医学

Mouse model suggests limited role for human mesotrypsin in pancreatitis

3区 · 医学

Article

作者: Miklós Sahin-Tóth ; Dóra Mosztbacher

Mesotrypsin is a low-abundance human trypsin isoform with a unique evolutionary mutation that conferred resistance to trypsin inhibitors and restricted substrate specificity. Mesotrypsin degrades the serine protease inhibitor Kazal type 1 (SPINK1) and thereby might increase risk for pancreatitis. Here, we report a mouse model designed to test the role of mesotrypsin in pancreatitis. We introduced the human mesotrypsin evolutionary signature mutation into mouse cationic trypsinogen (isoform T7), resulting in a Gly to Arg change at the corresponding position 199. In biochemical experiments using purified proteins, the p.G199R T7 mutant recapitulated all salient features of human mesotrypsin. T7G199R mice developed normally with no spontaneous pancreatitis or other obvious phenotypic changes. Cerulein-induced acute pancreatitis in C57BL/6N and T7G199R mice showed similar severity with respect to inflammatory parameters and acinar cell necrosis while plasma amylase activity was higher in T7G199R mice. Neither SPINK1 degradation nor elevated intrapancreatic trypsin activation was apparent in T7G199R mice. The results indicate that in T7G199R mice the newly created mesotrypsin-like activity has no significant impact on cerulein-induced pancreatitis. The observations suggest that human mesotrypsin is unimportant for pancreatitis; a notion that is consistent with published human genetic studies.

2021-01-01·Frontline Gastroenterology

How to manage: patient with a low faecal elastase

Article

作者: Lam, Kwan Wai ; Leeds, John

Pancreatic exocrine insufficiency (PEI) is underdiagnosed; however, the use of the faecal elastase-1 (Fel-1) test is common and recommended in the investigation of gastrointestinal symptoms. The Fel-1 test has been validated against a number of other tests of exocrine pancreatic function but often in very specific circumstances. If used routinely in gastrointestinal practice, an abnormal result will frequently occur but this cannot be assumed to mean that the patient has PEI. This finding should prompt the clinician to arrange further investigations to identify whether there is evidence of primary PEI, secondary PEI or whether there is another reason why the Fel-1 level is low. This review will provide an overview of Fel-1 testing, propose relevant investigations and discuss the potential pitfalls around management. Fel-1 is the most commonly utilised test in the UK for assessing exocrine pancreatic function and is recommended for investigation of chronic diarrhoea.1 2 Fel-1 is a proteolytic enzyme secreted by the exocrine pancreas and accounts for about 6% of total enzyme output.3 Elastase-1 binds to bile salts and minimally degraded during passage through the gastrointestinal tract and is chemically stable in faeces for up to a week at room temperature.4 Compared with direct tests, Fel-1 is simple to perform, non-invasive and more cost-effective.5 Multiple studies have shown good correlations between Fel-1 level and pancreatic enzyme output including amylase, trypsin and lipase.4–6 Fel-1 has been compared with other indirect tests and is superior to faecal chymotrypsin,2 4 6 faecal lipase,7 the 13C mixed triglyceride breath test8 and the pancreolauryl test.9 Most laboratories report an abnormal level as <200 µg/g stool; however, there are a number of caveats and pitfalls concerning a single sample and therefore a low level does not always indicate PEI. Borderline results (200–250 µg/g) should be considered …