1区 · 医学
ArticleOA
作者: Yao, Weiguo ; Lu, Shuaiyao ; A, Ruhan ; Zhan, Zhenzhen ; Zhang, Luyao ; Zhang, Yujian ; Li, Yuhua ; Huang, Baoying ; Liu, Zhongmin ; Li, Hangwen ; Niu, Peihua ; Wang, Wen ; Liu, Jingjing ; Zhao, Jincun ; Li, Shiqiang ; Wang, Wenling ; Tan, Wenjie ; Ma, Xiaopin ; Lin, Ang ; Zhao, Li ; Yang, Ren ; Huang, Lei ; Peng, Xiaozhong ; Liang, Xingjie ; Wang, Yufei ; Deng, Yao
Abstract:Although inoculation of COVID-19 vaccines has rolled out globally, there is still a critical need for safe and effective vaccines to ensure fair and equitable supply for all countries. Here, we report on the development of a highly efficacious mRNA vaccine, SW0123 that is composed of sequence-modified mRNA encoding the full-length SARS-CoV-2 Spike protein packaged in core–shell structured lipopolyplex (LPP) nanoparticles. SW0123 is easy to produce using a large-scale microfluidics-based apparatus. The unique core–shell structured nanoparticle facilitates vaccine uptake and demonstrates a high colloidal stability, and a desirable biodistribution pattern with low liver targeting effect upon intramuscular administration. Extensive evaluations in mice and nonhuman primates revealed strong immunogenicity of SW0123, represented by induction of Th1-polarized T cell responses and high levels of antibodies that were capable of neutralizing not only the wild-type SARS-CoV-2, but also a panel of variants including D614G and N501Y variants. In addition, SW0123 conferred effective protection in both mice and non-human primates upon SARS-CoV-2 challenge. Taken together, SW0123 is a promising vaccine candidate that holds prospects for further evaluation in humans.