Objective To establish UPLC fingerprints of Glycyrrhizae Radix et Rhizoma, and to provide a basis to develop the quality standard Methods RP-UPLC fingerprints were adopted, and Waters Acquity UPLC BEH C18(1.7 μm, 2.1 mm x 100 mm) was set as the chromatog. column. Acetonitrile-0.05% phosphoric acid was used as the mobile phase, for gradient elution; the flow rate was 0.4 mL/min; the detection wavelengths were set at 237 nm and 355 nm; the column temperature was kept at 30 °C; the sample volume was 2 μL; the recording time was 51 min. Results The methodol. findings of UPLC fingerprints met the tech. standard 24 fingerprint peaks were marked in Glycyrrhizae Radix et Rhizoma. 9 fingerprint peaks including glycyrrhizic acid, isoliquiritin apioside, licochalcone A, isoliquiritigenin, liquiritin, liquiritigenin, isoliquiritin, echinatin and glabridin were identified. The similarities of 11 batches of Glycyrrhizae Radix et Rhizoma were among 0.940-0.988 at 237 nm and among 0.831-0.982 at 355 nm. Conclusion The established UPLC fingerprints of Glycyrrhizae Radix et Rhizoma can provide basis for the establishment of its quality standard