ContextCervicitis is a common gynecol. inflammatory disease.The Chinese herbal prescription Kang-Gong-Yan (KGY) is clin. effective against cervicitis; however, the chem. constituents and therapeutic mechanism of KGY remain elusive.ObjectiveTo analyze the chem. constituents of KGY and explore the potential mechanism of KGY in treating cervicitis.Materials and methodsUHPLC-Q-Exactive Plus Orbitrap MS was used to identify the active compounds of KGY; Sprague-Dawley (SD) female rats were randomly divided into the control, model, and KGY groups.Phenol mucilage (25%) was slowly injected into the vagina and cervix of the rats to establish the cervicitis model.Then, rats in the KGY groups (low dose: 1 g/kg/d; medium dose: 5 g/kg/d; high dose: 10 g/kg/d) were continuously gavaged KGY for one week.HE staining was used to observe the cervical tissues of rats; ELISA was used to detect inflammatory factors in plasma; non-targeted metabolomics was used to analyze metabolites; 16S rRNA sequencing was used to analyze intestinal microorganisms.ResultsKGY exerted anti-cervicitis effects and decreased the levels of IL-6, IL-1β, and TNF-α.The mechanism of KGY in treating cervicitis is mainly associated with betaine, amino acid, pyrimidine, and phospholipid metabolism by regulating fifteen metabolites.Moreover, KGY reversed cervicitis-induced gut dysbiosis by mediating five bacteria.Discussion and conclusionsThe Chinese herbal prescription KGY may alleviate cervicitis by modulating metabolites and gut microbiota disorders.These findings provide a scientific basis for the clin. application of KGY and a new strategy for treating cervicitis in Chinese medicine.