Objective To explore the mechanism of tilianin in the treatment of coronary heart disease using network pharmacol. methods and mol. docking technol.Methods TCMSP database was used to obtain the biol. information of tilianin, and the Swiss target prediction website was established to predict the active mol. targets of tilianin, and the relationship network between tilianin and targets was constructed.Go(gene ontol.) anal. and KEEG pathway annotation anal. were performed by DAVID database.Used the drug bank database to search known targets of coronary heart disease with the key words of coronary heart disease, and using Venny 2.1.0(csic.es)website constructed the Venn map to screen the potential target and common target of tilianin.Autodock Vina 1.1.2 was used for mol. docking. and the conformation with the lowest binding energy of each docking result was used as the stable conformation.Through the establishment of a rat cardiomyocyte H9 c2 hypoxia-reoxygenation injury model, the efficacy of tilianin was exptl. verified.Results A total of 34 potential targets were predicted by Swiss target prediction website.Enrichment anal. showed that tilianin could inhibit cell apoptosis, inhibit calcium overload, activate vascular endothelial growth factor and promote cell proliferation.A total of 61 coronary heart disease targets were found through the database of drugbank, and 9 potential targets and coronary heart disease common targets were screened by constructing wenntu.Mol. docking showed tilianin has good binding ability to key targets such as CD38, NOX4, ADORA3, MMP1, ALDH2, AKR1 B1.CCK-8 results showed that tilianin could significantly promote the proliferation of H9 c2 injured by hypoxia reoxygenation.Conclusion Tilianin play a role in the treatment of coronary heart disease through multiple ways and targets.