The quality control method of quant. testing of endotoxin in rhGM-CSF was established.Kinetic chromogenic method was applied and quant. method of testing endotoxin in rhGM-CSF was established via reliability experiment of standard curve and interference experimentIn the reliability experiment of standard curve, R2 values of the curve were all above 0.98, which indicated the validity of the standard curve, in the interference experiment, the sample was redissolved and diluted five times before 0.5 EU/mL of standard endotoxin was added into the sample as recovered solutionThe recovery rates of endotoxin were all close to 100%.Under the dilution ratio, the interference of sample concentration was minor to the endotoxin experiment, in addition, the contents of endotoxin in two batches of samples were all lower than the limited value through routine testing, and the test results were consistent with that of gel method.The kinetic chromogenic method is applicable to the quant. testing of endotoxin in rhGM-CSF.