A rapid method to assess freshness is crucial for the safe handling of reconstituted infant formula (RIF) and prevention of food-borne illnesses in infants.This study explored the use of elec. impedance measurement (EIM) at a constant frequency for real-time freshness evaluation of RIF.Initial elec. impedance of 89.16 ± 0.47 Ω decreased to 74.29 ± 0.23 Ω within 48 h at room temperature (20 °C) storage.Under temperature abuse conditions (30 °C), the elec. impedance rapidly dropped from 75.48 ± 0.52 Ω to 60.15 ± 4.76 Ω within 18 h, indicating more rapid spoilage.Notable changes in the elec. impedance were observed during temperature abuse.Empirical model anal. revealed a strong pos. correlation between elec. impedance and conventional freshness parameters such as pH, protein, and microbial growth.This study demonstrates the potential of EIM for the rapid detection of freshness changes in RIF, offering valuable insights into the prevention of food-borne illnesses in infants.