更新于：2024-11-01

EEF1A1

更新于：2024-11-01

基本信息

别名

EE1A1、EEF1A、eEF1A-1

+ [13]

简介

Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis (PubMed:26651998, PubMed:26593721, PubMed:36264623, PubMed:36123449, PubMed:36638793). Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome (PubMed:26651998, PubMed:26593721, PubMed:36264623, PubMed:36123449, PubMed:36638793). The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation (PubMed:26651998, PubMed:26593721, PubMed:36264623, PubMed:36123449). Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed:17177976). (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection.

关联

项与 EEF1A1 相关的药物

Plitidepsin

靶点

EEF1A1

作用机制

EEF1A1抑制剂

在研机构

原研机构

在研适应症

非在研适应症

最高研发阶段批准上市

首次获批国家/地区

澳大利亚

首次获批日期2018-12-10

Malifutai

吗利福肽

靶点

EEF1A1 x eNOS

作用机制

EEF1A1抑制剂 [+1]

在研机构

八加一药业股份有限公司

原研机构

广东八加一医药有限公司

在研适应症

缺血性卒中 [+1]

非在研适应症-

最高研发阶段临床2期

首次获批国家/地区-

首次获批日期1800-01-20

NAI-Acne

靶点

EEF1A1

作用机制

EEF1A1抑制剂

在研机构-

原研机构

Pfizer Inc.

在研适应症-

非在研适应症

寻常痤疮

最高研发阶段无进展

首次获批国家/地区-

首次获批日期1800-01-20

项与 EEF1A1 相关的临床试验

EUCTR2022-002489-34-BE / Not yet recruiting临床2期

A Multicentre, Open label, Randomised, Controlled, Basket, Pragmatic, Phase II, Clinical and Translational Study to Determine the Efficacy and Safety of Plitidepsin versus Control in Immunocompromised Adult Patients with Symptomatic COVID-19 requiring Hospital Care (NEREIDA)

开始日期2023-05-30

申办/合作机构

Pharma Mar SA

NCT05705167 / Terminated临床2期

A Multicentre, Open Label, Randomised, Controlled, Basket, Pragmatic, Phase II, Clinical and Translational Study to Determine the Efficacy and Safety of Plitidepsin Versus Control in Immunocompromised Adult Patients With Symptomatic COVID-19 Requiring Hospital Care

The primary objective of this study is to evaluate efficacy of plitidepsin in pre-specified groups of immunocompromised patients with symptomatic COVID-19 requiring hospital care versus control in terms of mortality.

开始日期2023-04-19

申办/合作机构

Pharma Mar SA

NCT05121740 / Completed临床1/2期

Extension Study in a Cohort of Adult Patients With SARS-CoV-2 Infection Requiring Hospital Admission and Received Treatment With Plitidepsin in the APLICOV-PC Study

The main objective of this study is to evaluate the incidence of post-COVID morbidity and characterize the complications profile in patients who participated in the APLICOV-PC study. APLICOV-PC study was a multicenter, randomized, proof-of-concept clinical trial to assess the safety profile of 3 different dose levels of plitidepsin (1,5 mg, 2,0 mg and 2,5 mg) administered three consecutive days, in adult patients with confirmed diagnosis of COVID-19 who require hospital admission.

开始日期2022-01-18

申办/合作机构

Pharma Mar SA

[+1]

100 项与 EEF1A1 相关的临床结果

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100 项与 EEF1A1 相关的转化医学

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0 项与 EEF1A1 相关的专利（医药）

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2,390

项与 EEF1A1 相关的文献（医药）

2024-12-01·Computational and Structural Biotechnology Journal

Analysis of Haemonchus embryos at single cell resolution identifies two eukaryotic elongation factors as intervention target candidates

Article

作者: Taki, Aya C ; Young, Neil D ; Byrne, Joseph J ; Chang, Bill C H ; Korhonen, Pasi K ; Wang, Tao ; Gasser, Robin B ; Campos, Tulio L

Advances in single cell technologies are allowing investigations of a wide range of biological processes and pathways in animals, such as the multicellular model organism Caenorhabditis elegans - a free-living nematode. However, there has been limited application of such technology to related parasitic nematodes which cause major diseases of humans and animals worldwide. With no vaccines against the vast majority of parasitic nematodes and treatment failures due to drug resistance or inefficacy, new intervention targets are urgently needed, preferably informed by a deep understanding of these nematodes' cellular and molecular biology - which is presently lacking for most worms. Here, we created the first single cell atlas for an early developmental stage of Haemonchus contortus - a highly pathogenic, C. elegans-related parasitic nematode. We obtained and curated RNA sequence (snRNA-seq) data from single nuclei from embryonating eggs of H. contortus (150,000 droplets), and selected high-quality transcriptomic data for > 14,000 single nuclei for analysis, and identified 19 distinct clusters of cells. Guided by comparative analyses with C. elegans, we were able to reproducibly assign seven cell clusters to body wall muscle, hypodermis, neuronal, intestinal or seam cells, and identified eight genes that were transcribed in all cell clusters/types, three of which were inferred to be essential in H. contortus. Two of these genes (i.e. Hc-eef-1A and Hc-eef1G), coding for eukaryotic elongation factors (called Hc-eEF1A and Hc-eEF1G), were also demonstrated to be transcribed and expressed in all key developmental stages of H. contortus. Together with these findings, sequence- and structure-based comparative analyses indicated the potential of Hc-eEF1A and/or Hc-eEF1G as intervention targets within the protein biosynthesis machinery of H. contortus. Future work will focus on single cell studies of all key developmental stages and tissues of H. contortus, and on evaluating the suitability of the two elongation factor proteins as drug targets in H. contortus and related nematodes, with a view to finding new nematocidal drug candidates.

2024-10-01·Cell Stem Cell

Selective translation of nuclear mitochondrial respiratory proteins reprograms succinate metabolism in AML development and chemoresistance

Article

作者: Zhou, Fuling ; Liu, Zheming ; Zhao, Shanshan ; Wang, Jing ; Sun, Yan ; Zheng, Yanbing ; Lu, Pengbo ; Yin, Rong ; Song, Kehan ; Zhang, Haojian ; Zhang, Tiantian ; Han, Guoqiang ; Liu, Weidong ; Cao, Ziyan ; Guo, Rongxia ; Lin, Xiangpeng ; Hu, Jin ; Cui, Manman ; Gao, Kexin ; Shen, Min ; Li, Weiming ; Yao, Shuxin ; Chai, Jihua ; Tian, Wen ; Zhang, Yuhui

Mitochondrial adaptations dynamically reprogram cellular bioenergetics and metabolism and confer key properties for human cancers. However, the selective regulation of these mitochondrial responses remains largely elusive. Here, inspired by a genetic screening in acute myeloid leukemia (AML), we identify RAS effector RREB1 as a translational regulator and uncover a unique translation control system for nuclear-encoded mitochondrial proteins in human cancers. RREB1 deletion reduces mitochondrial activities and succinate metabolism, thereby damaging leukemia stem cell (LSC) function and AML development. Replenishing complex II subunit SDHD rectifies these deficiencies. Notably, inhibition of complex II re-sensitizes AML cells to venetoclax treatment. Mechanistically, a short RREB1 variant binds to a conserved motif in the 3' UTRs and cooperates with elongation factor eEF1A1 to enhance protein translation of nuclear-encoded mitochondrial mRNAs. Overall, our findings reveal a unique translation control mechanism for mitochondrial adaptations in AML pathogenesis and provide a potential strategy for targeting this vulnerability of LSCs.

2024-10-01·Plant Disease

First Report of Diaporthe tulliensis Causing Leaf Spot on Millettia speciosa in China

Article

作者: Liu, Qin ; Ya, Cai-Na ; Liu, Yao ; Geng, Xiao-Shan ; Pan, Jie-Ming ; Su, Ping-Ping ; Lai, Jie-Ling ; Jiang, Yuan-Yuan ; Bei, Yong-Jian ; Yu, Ye

Millettia speciosa Champ, renowned for its diverse applications in traditional medicine, is extensively cultivated in the Guangxi region of China, spanning roughly 5,973 hectares. In July 2021, a plantation in Yulin, Guangxi, China (22°64′N; 110°29′E), exhibited severe leaf spot disease on M. speciosa. Notably, a 46,690 square meters area had over 40% leaf spot incidence. Initially, symptoms appeared as small, circular, pale-yellow lesions on the leaves, then turned into irregular, dark brown spots with yellow halos, leading to the wilt and defoliation of leaves. To identify the responsible pathogen, a total of five symptomatic leaves were collected and sterilized systematically. Small tissue segments (5×5 mm) from lesion peripheries were aseptically excised, then surface sterilized with 75% ethanol for 10 s, and 1% sodium hypochlorite (NaClO) for 3 min. Following this, the sterilized tissues were triple-rinsed with sterile water and cultured on potato dextrose agar (PDA) at 28 °C in the dark for 7 d. A total of seven isolates were obtained through single-spore isolation, and one representative isolate, N2-3, was selected for further analysis. After 7 d of incubation, colonies displayed flat, white, and extensively branched aerial hyphae. Over time, the reverse side of the colony changed from white to yellowish-white. The pycnidia were black with conidial droplets ranging from cream to pale yellow exuding from their ostioles. The α-conidia were one-celled, hyaline, ovoid to cylindrical, typically with one or two droplets, 2.6 to 5.9 ×1.4 to 3.9 μm (n=50). These morphological traits align with those of the genus Diaporthe, as reported by Li et al. (2022) and Crous et al. (2015). To identify the species, isolate N2-3 underwent sequencing of the internal transcribed spacer (ITS), β-tubulin (BT), and translation elongation factor 1 alpha (EF1-α) sections (Huang et al. 2021). Obtained sequences of ITS, BT and EF1-α (Genebank accessions nos. OR600532, OR662169 and OR662168) displayed a 99% similarity to Diaporthe tulliensis (Genebank accessions nos. OP219651, ON932382, OL412437, respectively). Based on the concatenated ITS, BT and EF1-α, a neighbor-joining phylogenetic analyses using MEGA7.0 clustered with D. tulliensis. Therefore, the fungus was identified as D. tulliensis (teleomorph name) based on morphological and molecular features. A pathogenicity test was conducted on 1-year-old M. speciosa seedlings by gently abrading healthy leaves with sterilized toothpicks to create superficial wounds. Wounded leaves were then inoculated with 5 mm diameter mycelial plugs, while control seedlings received PDA plugs. Three leaves per plant and five plants per treatment were selected for assessment. All seedlings were kept in a controlled greenhouse (12/12h light/dark, 25 ± 2 °C, 90% humidity). After 7 d, the inoculated leaves showed symptoms like those in the field, while control plants remained healthy. The fungus was consistently reisolated from the infected leaves, satisfying Koch's postulates. Notably, D. tulliensis has caused Boston ivy leaf spot, bodhi tree leaf spot, cacao pod rot, and jasmine stem canker (Huang et al. 2021; Li et al. 2022; Serrato-Diaz et al. 2022; Hsu et al. 2023). This discovery is significant as it marks the first report of Diaporthe tulliensis causing leaf spot on Millettia speciossa in China, which has direct implications for the development of diagnostic tools and research into potential disease management strategies.

项与 EEF1A1 相关的新闻（医药）

2024-10-10

·生物探索

Nat Commun丨许文明、谭跃球联合揭示揭示中心体蛋白在男性不育发生过程中的新机制

引言精子发生是一个高度复杂的生物学过程，涉及精原细胞的增殖分化、减数分裂和精子细胞变形等过程。这一过程需要精确的基因表达调控，尤其是在精子变形期，由于染色质高度浓缩，转录活动逐渐停止，翻译后调控在这一阶段显得尤为重要。近年来，液-液相分离（LLPS）介导的生物分子凝聚体在调控细胞功能方面的作用引起了广泛关注。而在精子变形过程中，一个非常显著的事件是近端中心粒与远端中心粒的迁移与形成，其在精子发生与精子中心体形成中均具有关键作用，目前已鉴定出超过30多种的CEP蛋白，但其在精子变形与受精等过程中的作用尚未完全阐明。近日四川大学华西第二医院生殖遗传与表观遗传实验室许文明团队和中信湘雅生殖与遗传专科医院谭跃球团队合作在Nature Communications上发表了题为CEP112 Coordinates Translational Regulation of Essential Fertility Genes During Spermiogenesis Through Phase Separation in Humans and Mice的研究论文。这项研究揭示了中心体蛋白112（CEP112）在精子形成过程中的关键作用，并为男性不育的分子机制提供了新的见解。研究团队首先通过全外显子组测序在两例少弱畸形精子症患者中发现了CEP112基因的双等位基因变异。为深入了解CEP112的功能，研究人员构建了Cep112基因敲除小鼠模型。实验结果显示，Cep112缺失导致雄性小鼠不育，表现为精子数量减少、活力下降和形态异常。研究人员发现CEP112在成熟精子定位于精子的颈部和非典型中心粒，并在精子发育过程中形成RNA颗粒，富集了如Fsip2、Cfap61和Cfap74等调控精子发生的关键mRNA。通过多组学分析和实验验证，研究团队证明了CEP112调控目标mRNA的翻译。通过免疫共沉淀和质谱分析，他们确定了CEP112与翻译相关蛋白的相互作用，包括hnRNPA2B1、EEF1A1和EIF4A1。此外，FRAP与体外生化实验证实CEP112与hnRNPA2B1能够在体外发生液-液相分离，形成凝聚体并招募重要的翻译因子和其调控的mRNA。值得注意的是，患者来源的CEP112突变体显示出相分离能力的降低和翻译效率的下降，为理解CEP112突变导致不育的分子机制提供了线索。这些结果表明CEP112通过液-液相分离介导RNA颗粒的组装，从而控制男性生育关键基因的转录后表达。模式图（Credit: Nature Communications）本研究深入阐释了CEP112在精子发生中的关键角色，并拓展了我们对生物分子凝聚体调控生殖细胞功能的认识。继刘默芳团队2022年在Science发表FXR1通过相分离调控精子发生中mRNA翻译的研究之后，当前工作进一步突显了中心体蛋白可通过与精子鞭毛发育过程中的关键RNA结合，并通过液-液相分离机制发挥作用，为生殖生物学中相分离现象的理解奠定了基础。同时与临床相关的分析也发现，尽管Cep112的KO小鼠的ICSI的发育结局正常，CEP112基因突变患者在接受ICSI治疗后,出现胚胎发育停滞和种植失败。这一现象揭示了中心粒在小鼠和人类精子功能中的显著差异，表明其作用并非在物种间完全保守。研究结果强调了人类CEP蛋白在男性因素相关的早期胚胎发育中的关键作用。因此,对CEP相关基因突变的分析对于临床靶向检测和干预策略的制定具有重要意义。这些成果不仅为男性不育症的诊疗提供了新思路，也为针对男性不育的调控相分离过程的药物设计与RNA治疗策略设计均提供了可能的分子靶点与治疗思路。参考文献 https://doi.org/10.1038/s41467-024-52705-8 责编|探索君排版|探索君文章来源|“BioArt” End 往期精选围观一文读透细胞死亡(Cell Death) | 24年Cell重磅综述（长文收藏版）热文 Cell | 是什么决定了细胞的大小？热文 Nature | 2024年值得关注的七项技术热文 Nature | 自身免疫性疾病能被治愈吗？科学家们终于看到了希望热文 CRISPR技术进化史 | 24年Cell综述

信使RNA

2024-10-02

·生物探索

CSC丨张好建/周芙玲/孙妍团队合作揭示核编码线粒体蛋白的独特翻译控制系统及其在急性髓系白血病发生发展与耐药中的重要作用

引言线粒体是细胞能量代谢中心和系统信号中枢，具有强大适应能力，以满足各种生理病理条件下能量需求，确保细胞存活【1】。急性髓系白血病（AML）是最为常见的成人白血病，由造血干祖细胞恶性转化的白血病干细胞发展而来。目前，AML（急性早幼粒细胞白血病除外）治疗手段有限、复发率高、死亡率高，5年总体生存率低<30%。线粒体适应性在AML发生发展及耐药中发挥着重要作用【2】。绝大多数的线粒体蛋白是细胞核DNA编码，这些核编码线粒体蛋白是如何被选择性调控的，目前却知之甚少。 2024年10月1日，武汉大学医学研究院/免疫与代谢前沿科学中心/口腔医学院/泰康生命医学中心张好建课题组，武汉大学中南医院周芙玲课题组，和广州省人民医院/广东省医学科学院孙妍课题组在Cell Stem Cell杂志上发表了题为：Selective translation of nuclear mitochondrial respiratory proteins reprograms succinate metabolism in AML development and chemoresistance的研究论文。该研究发现了RAS响应元件结合蛋白RREB1促进AML发生发展的重要作用，揭示了其同源异构体RREB1S选择性调控核编码线粒体蛋白翻译，促进琥珀酸代谢和线粒体活性，进而维持白血病干细胞干性和药物抗性的分子机制。造血干细胞往往通过严格的翻译控制来维持干性和静息状态【3】。在前期研究中，张好建课题组解析了正常造血干细胞恶变为白血病干细胞的染色质变化和RNA m6A修饰重塑规律，揭示了AML发生的表观驱动力【4-6】。然而，AML发生过程的蛋白翻译控制体系如何变化？聚焦此科学问题，研究者们利用OP-Puro定量分析蛋白合成速率，并结合染色质变化规律和基因筛选，鉴定出了RREB1为潜在的蛋白翻译调控因子。采用条件性基因敲除小鼠和AML模型等，证实了RREB1调控AML发生发展、维持白血病干细胞干性和功能的重要作用。进一步探索发现RREB1缺失降低线粒体呼吸链复合物相关亚基的蛋白翻译，损伤白血病干细胞线粒体活性和代谢体系；其中复合物II受损最为显著，同时该复合物介导的琥珀酸代谢受阻，进而损害白血病干细胞干性和功能；回补复合体 II 亚基 SDHD或者其下游代谢产物延胡索酸和辅酶Q10 可以恢复这些表型。有意思的是，研究者发现了一个新的RREB1同源异构体，将其命名为RREB1S。RREB1S缺失核定位序列，改变了其在细胞内的空间定位和功能，使其可以特异增强白血病细胞和多种肿瘤细胞的蛋白翻译速率。在机制上，研究者们在核DNA编码的线粒体呼吸链基因的3’UTR区域发现了一段保守的翻译增强序列，并证明了RREB1S可以特异识别并结合该段序列，招募翻译延长因子eEF1A1，进而促进核编码线粒体呼吸链复合物相关亚基的蛋白翻译。另外，研究者们还发现RREB1S的高表达导致AML细胞对临床药物BCL2抑制剂——维奈克拉（Venetoclax）的抗性，抑制复合物II的活性可恢复耐药AML细胞对维奈克拉的敏感性。模式图（Credit: Cell Stem Cell）综上所述，该研究发现了一种新的核编码线粒体蛋白的翻译控制系统，揭示了线粒体适应性调控AML发生发展与耐药的作用与机制，为靶向白血病干细胞和克服耐药提供了新治疗策略。参考文献 1. Faubert, B., Solmonson, A., and DeBerardinis, R.J. (2020). Metabolic reprogramming and cancer progression. Science 368, eaaw5473. 10.1126/science.aaw5473. 2. Lagadinou, E.D., Sach, A., Callahan, K., Rossi, R.M., Neering, S.J., Minhajuddin, M., Ashton, J.M., Pei, S.S., Grose, V., O'Dwyer, K.M., et al. (2013). BCL-2 Inhibition Targets Oxidative Phosphorylation and Selectively Eradicates Quiescent Human Leukemia Stem Cells. Cell Stem Cell 12, 329-341. 10.1016/j.stem.2012.12.013. 3. Signer, R.A.J., Magee, J.A., Salic, A., and Morrison, S.J. (2014). Haematopoietic stem cells require a highly regulated protein synthesis rate. Nature 509, 49-54. 10.1038/nature13035. 4. Wang, J., Li, Y., Wang, P., Han, G., Zhang, T., Chang, J., Yin, R., Shan, Y., Wen, J., Xie, X., et al. (2020). Leukemogenic Chromatin Alterations Promote AML Leukemia Stem Cells via a KDM4C-ALKBH5-AXL Signaling Axis. Cell stem cell 27, 81-97.e88. 10.1016/j.stem.2020.04.001. 5. Yin, R., Chang, J., Li, Y., Gao, Z., Qiu, Q., Wang, Q., Han, G., Chai, J., Feng, M., Wang, P., et al. (2022). Differential m6A RNA landscapes across hematopoiesis reveal a role for IGF2BP2 in preserving hematopoietic stem cell function. Cell stem cell 29, 149-159.e147. 10.1016/j.stem.2021.09.014. 6. Cheng, Y., Gao, Z.Y., Zhang, T.T., Wang, Y.H., Xie, X.Q., Han, G.Q., Li, Y.S., Yin, R., Chen, Y.L., Wang, P.P., et al. (2023). Decoding m6A RNA methylome identifies PRMT6-regulated lipid transport promoting AML stem cell maintenance. Cell stem cell 30, 69-85. 10.1016/j.stem.2022.12.003. https://www.cell.com/cell-stem-cell/abstract/S1934-5909(24)00322-9 责编|探索君排版|探索君文章来源|“BioArt” End 往期精选围观一文读透细胞死亡(Cell Death) | 24年Cell重磅综述（长文收藏版）热文 Cell | 是什么决定了细胞的大小？热文 Nature | 2024年值得关注的七项技术热文 Nature | 自身免疫性疾病能被治愈吗？科学家们终于看到了希望热文 CRISPR技术进化史 | 24年Cell综述

2023-09-12

·生物谷

中山大学研究者们揭示了纳米粒介导的lncBCMA沉默促进eEF1A1泛素化并抑制乳腺癌生长和转移

随着医学技术的快速发展，多年来癌症的诊断和治疗取得了巨大的成就。然而，转移仍然发生在许多癌症患者中，并占癌症相关死亡的90%。随着医学技术的快速发展，多年来癌症的诊断和治疗取得了巨大的成就。然而，转移仍然发生在许多癌症患者中，并占癌症相关死亡的90%。在过去的十年里，尽管由于早期诊断的快速发展，癌症患者的存活率得到了显著的提高，但在转移性癌症的治疗方面却取得了有限的成就。目前，化疗和靶向治疗仍是转移性癌症患者的一线治疗方式。然而，耐药性和严重的毒副作用严重制约了治疗结果。因此，了解肿瘤转移的生物学过程，找出导致肿瘤转移的关键调控因子，对于制定有效的治疗策略至关重要。图片来源：https://doi.org/10.1016/j.apsb.2022.12.004 近日，来自中山大学的研究者们在Acta Pharmaceutica Sinica B杂志上发表了题为“Nanoparticles (NPs)-mediated lncBCMA silencing to promote eEF1A1 ubiquitination and suppress breast cancer growth and metastasis”的文章，该研究揭示了纳米粒介导的lncBCMA沉默促进eEF1A1泛素化并抑制乳腺癌生长和转移。长非编码RNA(LncRNAs)在肿瘤转移中起重要作用。探索转移相关的lncRNA，开发有效的体内靶向调控lncRNA功能的策略，对于转移性肿瘤的治疗至关重要，但这仍然是一个巨大的挑战。在本研究中，研究者发现了一种新的功能性lncRNA(记为lncBCMA)，它可以通过拮抗真核细胞翻译延长因子1A1(EEF1A1)的泛素化来促进三阴性乳腺癌(TNBC)的生长和转移，从而稳定其表达。基于这一调控机制，设计了一种可用于系统递送lncBCMA siRNA(SiBCMA)的内体pH响应型纳米颗粒(NP)平台。这种NPs介导的siBCMA可以有效地沉默lncBCMA的表达，促进eEF1A1的泛素化，从而显著抑制TNBC肿瘤的生长和转移。这些结果表明，lncBCMA可作为预测TNBC患者预后的潜在生物标志物，NPs介导的lncBCMA沉默可能是转移性TNBC治疗的一种有效策略。 NPS介导的LncBCMA沉默抑制PDX肿瘤模型中TNBC的生长和转移图片来源：https://doi.org/10.1016/j.apsb.2022.12.004 综上所述，我们发现了一种新的具有功能的lncRNA(即lncBCMA)，它在调节TNBC肿瘤的生长和转移中发挥重要作用。分子机制研究表明，该lncRNA可以通过拮抗其泛素化来稳定eEF1A1的表达，从而促进TNBC细胞的增殖、迁移和侵袭。利用胞体pH响应型纳米粒体内携带siBCMA，可以有效地抑制lncBCMA的表达，促进eEF1A1泛素化，从而显著抑制肿瘤的生长和转移。NncBCMA可用于预测TNBC患者的预后，NPs介导的lncBCMA沉默可能是治疗转移性TNBC的一种有前途的策略。（生物谷 Bioon.com）参考文献 Ke Yang et al. Nanoparticles (NPs)-mediated lncBCMA silencing to promote eEF1A1 ubiquitination and suppress breast cancer growth and metastasis. Acta Pharm Sin B. 2023 Aug;13(8):3489-3502. doi: 10.1016/j.apsb.2022.12.004.