更新于：2024-11-01

细菌群体感应蛋白

更新于：2024-11-01

基本信息

别名-

简介-

关联

靶点

细菌群体感应蛋白

作用机制

细菌群体感应蛋白抑制剂

在研机构

山东大学

原研机构

山东大学

在研适应症

细菌感染

非在研适应症-

最高研发阶段临床前

首次获批国家/地区-

首次获批日期1800-01-20

EDP-5

靶点

细菌群体感应蛋白

作用机制

细菌群体感应蛋白抑制剂

在研机构-

原研机构

BioLineRx Ltd.

在研适应症-

非在研适应症

细菌感染

最高研发阶段无进展

首次获批国家/地区-

首次获批日期1800-01-20

100 项与细菌群体感应蛋白相关的临床结果

登录后查看更多信息

100 项与细菌群体感应蛋白相关的转化医学

登录后查看更多信息

0 项与细菌群体感应蛋白相关的专利（医药）

登录后查看更多信息

项与细菌群体感应蛋白相关的文献（医药）

2021-08-01·3 Biotech4区 · 工程技术

Tryptophan interferes with the quorum sensing and cell surface hydrophobicity of Staphylococcus aureus: a promising approach to inhibit the biofilm development

4区 · 工程技术

Article

作者: Mandal, Sukhendu ; Das, Amlan ; Dastidar, Debabrata Ghosh ; Sarker, Ranojit K ; Chakraborty, Poulomi ; Tribedi, Prosun ; Paul, Payel ; Bhattacharjee, Surajit ; Chatterjee, Ahana ; Maiti, Debasish

Staphylococcus aureus, a Gram-positive bacterium has been implicated in a plethora of human infections by virtue of its biofilm-forming ability. Inhibition in microbial biofilm formation has been found to be a promising approach towards compromising microbial pathogenesis. In this regard, various natural and synthetic molecules have been explored to attenuate microbial biofilm. In this study, the role of an amino acid, L-tryptophan was examined against the biofilm-forming ability of S. aureus. The compound did not execute any antimicrobial characteristics, instead, showed strong antibiofilm activity with the highest biofilm inhibition at a concentration of 50 µg/mL. Towards understanding the underlying mechanism of the same, efforts were given to examine whether tryptophan could inhibit biofilm formation by interfering with the quorum-sensing property of S. aureus. A molecular docking analysis revealed an efficient binding between the quorum-sensing protein, AgrA, and tryptophan. Moreover, the expression of the quorum-sensing gene (agrA) got significantly reduced under the influence of the test compound. These results indicated that tryptophan could interfere with the quorum-sensing property of the organism thereby inhibiting its biofilm formation. Further study revealed that tryptophan could also reduce the cell surface hydrophobicity of S. aureus by downregulating the expression of dltA. Moreover, the tested concentrations of tryptophan did not show any significant cytotoxicity. Hence, tryptophan could be recommended as a potential antibiofilm agent to manage the biofilm-associated infections caused by S. aureus.SUPPLEMENTARY INFORMATIONThe online version contains supplementary material available at 10.1007/s13205-021-02924-3.

2003-07-08·Proceedings of the National Academy of Sciences1区 · 综合性期刊

Use of in vivo -induced antigen technology (IVIAT) to identify genes uniquely expressed during human infection with Vibrio cholerae

1区 · 综合性期刊

Article

作者: Asaduzzaman, Muhammad ; Vanderspurt, Cecily ; Bridges, Emily Anna ; Kirn, Thomas J. ; Taylor, Ronald K. ; Ryan, Edward T. ; Progulske-Fox, Ann ; Hillman, Jeffrey D. ; Calderwood, Stephen B. ; Hang, Long ; Handfield, Martin ; John, Manohar

International Journal of Molecular Sciences2区 · 生物学

Intra-Species Bacterial Quorum Sensing Studied at Single Cell Level in a Double Droplet Trapping System

2区 · 生物学

ArticleOA

作者: Jie Pan ; Martin Welch ; Steven D. Bowden ; Chris Abell ; Simon Poulter ; Yunpeng Bai ; Wilhelm T. S. Huck ; Santoshkumar N. Patil ; George P. C. Salmond

In this paper, we investigated the intra-species bacterial quorum sensing at the single cell level using a double droplet trapping system. Escherichia coli transformed to express the quorum sensing receptor protein, LasR, were encapsulated in microdroplets that were positioned adjacent to microdroplets containing the autoinducer, N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL). Functional activation of the LasR protein by diffusion of the OdDHL across the droplet interface was measured by monitoring the expression of green fluorescent protein (GFP) from a LasR-dependent promoter. A threshold concentration of OdDHL was found to induce production of quorum-sensing associated GFP by E. coli. Additionally, we demonstrated that LasR-dependent activation of GFP expression was also initiated when the adjacent droplets contained single E. coli transformed with the OdDHL synthase gene, LasI, representing a simple quorum sensing circuit between two droplets.