AIM To establish the HPLC fingerprints of Panax notoginseng(Burk.) F. H. Chen and to determine the contents of five constituents.METHODS The anal. of the 70% methanol extract from P. notoginseng was developed on a 25 °C thermostatic Waters XBridge C_(18 )column(4.6 mmx250 mm,5 μm), with the mobile phase comprising of acetonitrile-water flowing at 1.5 mL/min in a gradient elution manner, and the detection wavelength was set at 203 nm.RESULTS There were five common peaks in the fingerprints of fifteen batches of samples, with the similarities of more than 0.99.Notoginsenoside R_1, ginsenoside Rg_1, ginsenoside Re, ginsenoside Rb_(1 )and ginsenoside Rd showed good linear relationships within the ranges of 0.000 76-0.567 64(r=0.999 9),0.002 69-2.017 43(r=0.999 8),0.000 38-0.283 82(r=1.000 0),0.002 83-2.124 83(r=0.999 8),0.000 78-0.582 98 mg/mL(r=0.999 8),whose average recoveries were 101.78%,97.22%,102.14%,98.96%,101.73% with the RSDs of 1.58%,1.31%,2.17%,1.55%,1.80%,resp.CONCLUSION This stable and reliable method can be used for the quality control of P. notoginseng.