Objective: To detect the content of menthol in the export Jingwanhong Ointment. Methods: The gas chromatog. (GC method) was capillary column that using polyethylene glycol 20000 (PEG-20M) as the stationary phase (column was 30 m long, internal diameter of column was 0.32 mm, film thickness was 0.25 μm), and inlet temperature was 220°C, flame ionization detector temperature was 250°C, the column temperature was 110°C, the split ratio was 5:1. Results: The content of menthol in the range of 0.04-0.4 μg had a good linear correlation, coefficient r=0.9998, and the average recovery was 98.76%, RSD=1.43%. Conclusion: GC method is reproducible, simple and reliable that can be used for detecting the content of menthol in the export Jingwanhong Ointment.