The sensitive simultaneous detection of multiplex biomarkers is critical for the early cancer diagnosis. Herein, a dual potential- and waveband-resolved electrochemiluminescence (ECL) platform is reported for multiplexing immunoassay, using a rotating band-pass filter array for ECL spectrum discrimination. Three lung cancer biomarkers, neuron-specific enolase (NSE), carcinoembryonic antigen (CEA) and cytokeratin 19 fragment antigen (CYFRA21-1) were adopted as model analytes, with nanocomposites of gold nanoparticles/graphene oxide/N, N′- dicaproate sodium-3,4,9,10-perylenedicarboximide (AuNPs/GO/PDI), CdSe nanocrystals (NCs) and CdTe NCs as ECL tags, resp. After forming corresponding sandwich-type immune-complexes on the surface of modified electrode via an one-pot immune-reaction, the intensities from the ECL tags of AuNPs/GO/PDI (Ep = -0.41∼ -0.46 V), CdSe NCs (λmax = 550 nm, Ep = -1.44∼ -1.46 V), and CdTe NCs (λmax=750 nm, Ep = -1.49 ∼ -1.51 V) in the signal antibody bio-conjugates were measured in a single cathodic potential scan run with K2S2O8 as the coreactant. The interference from the ECL of O2/S2O2-8 enhanced by the three ECL tags was corrected by the intensity at 649 nm in the waveband-resolved ECL mode. Under optimized conditions, NSE, CYFRA21-1 and CEA were simultaneously quantified with the detection limit values of 0.86, 2.6 and 0.53 fg mL-1, resp.