A high-performance liquid chromatog. (HPLC) method was established for the determination of chlorogenic acid content in Shuanghuanglian effervescent tablets.After treated with methanol, the samples were determined by using C18 column (200 mm × 4.6 mm, 5 μm) with the mobile phase of methanol-water-glacial acetic acid (15:85:1) under detection wavelength at 324 nm.Within the injection volume range of 0.3-1.8 μg, the injection volume and peak area showed a good linear relationship, the recovery of this method reached 99.68% with RSD=0.74%.The method was specific, accurate and repeatable for determination of chlorogenic acid in Shuanghuanglian effervescent tablets.