[Objective] To investigate effects of hyperoside on the antioxidant capability and immune function of mice. [Method] The BALB/c mice were randomly divided into 5 groups: blank control group, immunosuppressed group, drug group, pos. control group. Then the level of MDA, H2O2, thymus index, spleen index T-AOC, T-SOD, MPO, XOD, GSH-PX and POD were measured. [Result] The expression of thymus index, spleen index, T-AOC, T-SOD, MPO, GSH-PX and POD decreased significantly, and the level of MDA, H2O2 and XOD increased significantly compared with blank control group. After treatment with hyperoside, the level of thymus index, spleen index, T-AOC, T-SOD, MPO, GSH-PX and POD increased significantly, while the level of MDA, H2O2 and XOD reduced significantly compared with the immunosuppressed group. [Conclusion] Hyperoside could improve the antioxidant capability and immune function by increasing the level of thymus index, spleen index, T-AOC, T-SOD, MPO, GSH-PX and POD and decreasing the level of MDA, H2O2, and XOD in immunosuppressed mice.