Allergic rhinitis is a common chronic non-infectious inflammatory disease of the upper respiratory tract, which affects patients' health, quality of life and work efficiency for a long time. Clinical manifestations are mainly nasal congestion, paroxysmal sneezing, clear watery nasal discharge and nasal itching, among which nasal congestion caused by nasal mucosal edema is the main manifestation of allergic rhinitis. Nasal mucosa has a rich network of arteriovenous anastomosis, and there are many large venous sinuses constitute a spongy structure, with a strong capacity for expansion and contraction, in the onset of allergic rhinitis, the volume of venous sinuses in the nasal mucosa can be rapidly changed, venous sinus expansion and filling can be induced by nasal mucosal congestion and nasal mucosal edema nasal congestion symptoms. Aescine sodium has been widely used in the clinical treatment of local tissue edema and the effect is remarkable, a large number of clinical results have proved that it has a significant anti-inflammatory, anti-edema, enhance the venous tension, improve blood circulation and anti-allergic effect, can be improved through the improvement of the vascular endothelial cell barrier, and enhance the epithelial cells close connection to improve the local edema and acute inflammation. The current treatment strategies for allergic rhinitis mainly include environmental control, medication, immunotherapy and health education, however, some patients are still unable to effectively control their symptoms in the long term after standardized treatment, and nasal congestion and runny nose cause great disturbance to the quality of their daily life. Therefore, it is urgent to investigate the mechanism of aescine sodium in relieving nasal mucosal edema in allergic rhinitis.

开始日期2024-12-01

申办/合作机构

南京医科大学第一附属医院

ChiCTR2200061992

/ Suspended临床4期IIT

Study on the efficacy and safety of sodium aescinate tablets (Oukai) in the treatment of postoperative soft tissue edema in patients with limbs closed fracture

开始日期2022-08-15

申办/合作机构

南昌大学第一附属医院

NCT05263167

/ Not yet recruiting临床4期IIT

Reducing Edema After intraCerebral Hemorrhage

The REACH trial is a prospective multicenter double-blind randomized placebo-controlled trial with blinded end-point adjudication. Participants are randomized (1:1) to receive either sodium aescinate or matching placebo (0.9% saline). The primary outcome is the absolute volume of PHE evaluated based on brain CT image on day 14 after ICH.

开始日期2022-03-15

申办/合作机构

北京天坛医院

100 项与 PGF2α 相关的临床结果

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100 项与 PGF2α 相关的转化医学

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0 项与 PGF2α 相关的专利（医药）

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项与 PGF2α 相关的文献（医药）

2025-08-01·The Veterinary Journal

Development of a cell culture system from bovine and bubaline endometrial cells recovered by a minimally invasive technique using a cytobrush

Article

作者: Swangchan-Uthai, Theerawat ; Sirivaidyapong, Sudson ; Jongsuwanwattana, Ruchikon

Ruminant endometrial cell culture is a common model system used to study uterine receptivity and related signaling in the maternal endometrium and pregnancy. The present study aimed to develop a minimally invasive technique by employing a cytobrush for collecting endometrial cell samples retrieved from cattle and buffalo and to establish and characterize the cells. The study was divided into two experiments. Experiment I evaluated the tissue invasiveness associated with different brush cytology rotations for 3, 5, 7, and 9 rounds compared with biopsy samples. In total, 30 areas of endometrial tissue samples from the uteri of six fresh, healthy, non-pregnant bovines from a slaughterhouse were examined histologically to determine tissue invasiveness based on the perpetual endometrial layer. The seven rounds of cytobrush rotation provided optimal results with detached epithelial cell lining and sloughed-off basement membrane while maintaining intact endometrial stroma, suggesting its suitability for subsequent cell culture. In Experiment II, primary endometrial cell cultures were established employing the 7-round brush cytology method from ten healthy, Holstein Friesian and swamp buffalo cows (n = 5, each). Endometrial cells were subcultured up to the third passage for further validation. Physical characterization utilized immunocytochemistry with pan-cytokeratin and vimentin co-staining. The cultured cells were functionally assessed by quantifying prostaglandin F2 alpha (PGF2α) secretion following 0 and 100 nM oxytocin challenge. Our findings showed that cytobrush sampling yielded sufficient seeding cells for culture. The proportions of endometrial epithelial cells relative to the stromal cells at passage 3 were 89.98 ± 7.21 % and 85.18 ± 2.66 % for cows and buffaloes, respectively. The secretion of PGF2α at 24 h increased significantly in bovine endometrial cell culture with 100 nM oxytocin (931.37 ± 292.69 pg/mL) compared with 0 nM oxytocin (194.06 ± 43.95 pg/mL; p = 0.026), as well as in bubaline endometrial cell culture, at 100 nM oxytocin (5.17 ± 1.54 pg/mL) compared 0 nM oxytocin (1.92 ± 0.76 pg/mL; p > 0.05). In conclusion, minimally tissue-invasive in vivo brush cytology sampling methods are effective for establishing a primary endometrial cell culture system in cows and buffaloes, providing a valuable model for studying reproductive physiology.

2025-07-01·Domestic Animal Endocrinology

Effectors and predictors of conceptus survival in cattle: What is next?

Article

作者: Silva, Felipe A C C ; Lopez-Duarte, Maria Camila ; Rocha, Cecilia Constantino ; Gonella-Diaza, Angela ; Martins, Thiago ; Binelli, Mario ; Pugliesi, Guilherme

In cattle, the physiological process of switching from cycling to pregnant is complex. Ultimately, that process relies on endometrial luminal epithelial cells and is based on the paracrine context of the uterine lumen. Cells either release luteolytic pulses of prostaglandin F2 alpha to keep the animal cycling or respond to cues released by the elongated conceptus that block prostaglandin F2 alpha pulses to maintain luteal function and pregnancy. That process, however, is highly regulated and subjected to error that occurs in every 30 to 40 % of attempted bovine pregnancies. This review addresses novel aspects of endometrial cell function, luteal function, intraluminal endometrial-trophoblast communication, heat stress, and artificial intelligence as effectors, predictors, and tools to be explored and employed to reduce pregnancy loss.

2025-06-01·Cytokine

The synergistic effects of prostaglandin and IL-1β on myometrial and cervical stromal cells at the onset of labor

Article

作者: Huang, Qian ; Liu, Huishu ; Li, Pin ; Sha, Xiaoyan ; Jiang, Yanmin ; Wang, Lele ; Lin, Baohua ; Zheng, Zheng ; Bao, Junjie

Inflammatory cytokines such as IL-1β and prostaglandins (PGs) are pivotal in the initiation of labor. Nevertheless, the synergistic interaction between PGs and IL-1β remains to be fully elucidated. Labor is defined as regular and gradually increasing uterine contractions accompanied by progressive dilation of the cervix, and descent of the fetal. This study employed Luminex to monitor alterations in inflammatory cytokine levels within myometrial tissue (n = 10) during labor compared to non-labor (n = 10) conditions. And the synergistic relationship between PGs and IL-1β by investigating the primary myometrium cells and cervical stromal cells culture. The results showed that the inflammatory cytokines of IL-1β, IL-6, IL-8 and TNF-α in the myometrium tissue were increased in labor group. In myometrium cells, PGF2α and IL-1β synergistically up-regulated COX-2 mRNA, upregulated the transcription of PRA and PRB, PGF2α alleviated that IL-1β up-regulated IL-8 mRNA. In cervical stromal cells, IL-1β up-regulated the COX-2 and PRB protein expression. PGE2 abated that IL-1β up-regulated IL-8 mRNA. PGE increased the expression of PRs, which is more pronounced with the prolonged duration. Ratio of PRA/PRB show an increased trend with IL-1β and PGE2 co-regulated. This study further clarified the synergistic regulatory mechanism of IL-1β and PGs, offering a theoretical foundation for the development of strategies aimed at labor induction and the prevention and treatment of preterm birth.

项与 PGF2α 相关的新闻（医药）

2024-12-14

·药时代

最新成果|PNAS|徐华强/吴灿荣团队揭示前列腺素D2受体DP2的分子机制

前列腺素是通过环氧合酶（COX）途径合成的生物活性脂质，在炎症、免疫反应和多种细胞过程中发挥着重要作用。它们通过与特定的脂质G蛋白偶联受体相互作用，诱发多种生物功能，包括前列腺素D2受体DP1和DP2、前列环素受体IP、前列腺素E2受体EP1至EP4、前列腺素F2α受体FP以及血栓素受体TP。前列腺素D2（PGD2）主要在中枢神经系统和免疫系统中产生，并在睡眠调节和炎症反应中发挥重要作用。DP2，也被称为T辅助细胞2型表达的趋化因子受体同源分子（CRTH2），在过敏性疾病中尤为重要。尽管DP2与其他前列腺素受体的序列同源性较低，但它与趋化因子受体的关系密切，主要在T辅助细胞2型、嗜酸性粒细胞等免疫细胞中表达。DP2受体通过与Gi亚型G蛋白偶联，调节cAMP和Ca2+的水平，并能与β-arrestin蛋白相互作用。研究团队发现，PGD2主要通过Gαi信号通路激活DP2，而吲哚美辛则倾向于通过β-arrestin蛋白信号通路进行激活 (图1A-C)。利用冷冻电子显微镜（cryo-EM）技术，团队成功解析了三种DP2-Gi复合物的结构，包括未结合配体的DP2-Gi复合物、结合内源性配体PGD2的复合物，以及结合吲哚美辛的复合物（图1D-F）。这些结构揭示了PGD2与吲哚美辛在DP2上的不同结合模式，为理解受体激活和信号转导提供了关键见解（图1G）。通过结构分析结合功能实验，研究深入探讨了这两种分子独特的受体激活机制，并揭示了吲哚美辛偏向性激活β-逮捕蛋白的分子机制（图1H-J）。此外，研究还发现了一个新的磷脂结合位点，该位点位于DP2与G蛋白的交互界面。结合质谱分析、G蛋白招募实验、AlphaScreen结合力测定以及分子动力学模拟，研究表明该磷脂类在调节DP2信号转导中发挥了重要作用 (图1K-N)。这一发现不仅丰富了对DP2功能的理解，也为未来的药物开发提供了新的思路。图1. (A) 吲哚美辛和PGD2的化学结构。(B) 吲哚美辛和PGD2对DP2激活的pEC50值。(C) 人DP2受体信号偏向的示意图。(D, E, F) PGD2-DP2-Gi复合物、吲哚美辛-DP2-Gi复合物和DP2-Gi复合物的冷冻电镜密度图。(G) PGD2、吲哚美辛和W2596.48之间的距离测量。(H) 吲哚美辛、fevipiprant及参与受体激活的关键残基的位置。(I) 吲哚美辛结合的DP2中吲哚美辛与非活性DP2中F2947.43之间的冲突。(J) 吲哚美辛结合的DP2与非活性DP2的叠加，二者在TM6和TM7的中点对齐；突出显示了吲哚美辛结合的DP2中的N296与非活性DP2中TM6骨架之间的冲突。(K) DP2-Gi与16:0-18:1 PI之间的相互作用界面；残基以棒状表示。(L) 质谱分析结果。(M) 野生型DP2与突变体之间Gi招募效率的比较。(N) AlphaScreen相互作用测定结果，显示在不同磷脂存在下，10 nM纯化的生物素-Gi与10 nM H8标记的DP2蛋白的招募。该团队此前已报道前列腺素F2α（Nature Communications, 2023）和前列环素受体（Science Advances, 2024）的结构药理学机制，当前研究是在前列腺素受体结构药理学领域的又一重要突破。研究团队希望通过进一步探索，推动DP2相关药物的临床应用，为过敏性疾病患者带来新的治疗选择。随着对DP2机制的深入了解，科学家们期待能够设计出更具针对性和安全性的抗过敏性哮喘药物，从而改善患者的生活质量。本研究中的冷冻电镜数据在上海市高峰电镜中心收集。上海科技大学的徐久银、上海药物研究所的徐有伟副研究员、南京中医药大学的博士生侯丽和上海药物研究所博士生何欣恒为该论文的共同第一作者；上海药物所的徐华强研究员和吴灿荣副研究员为共同通讯作者。上海药物所为本研究的第一完成单位。清华大学瑕瑜教授和临港实验室蒋轶研究员对此研究做出了重要贡献。该工作获得了国家自然科学基金委、科技部、中科院先导专项以及上海市市级科技重大专项等项目的资助。 Nature Reviews Endocrinology 综述丨徐华强/段佳团队概述冷冻电镜在GPCR药物发现中的突破性研究 Nature |上海药物所徐华强课题组与合作者共同揭示“冰毒”与其受体TAAR1的分子识别机制 Cell Research | 徐华强团队合作揭示多巴胺受体系统介导药物分子多重药理学活性的结构基础 Nat Chem Bio | 上海药物研究所庄友文/徐华强揭示过敏毒素识别补体受体系统的分子模式和选择性机理 NSMB | 徐华强/谢欣/蒋轶发现孤儿受体GPR119内源性配体的分子机制首发 | 中科院徐华强/尹万超团队联合攻关新冠病毒奥密克戎（Omicron）变异株刺突蛋白及其与受体和抗病毒抗体的复合物结构点击这里，查看更多精彩！

2024-09-12

·生物谷

碳酸饮料成“罪魁祸首”？！Sci. Rep. | 饮用碳酸饮料的痛经发生率比不饮用碳酸饮料的高1.24倍

原发性痛经（Primary dysmenorrhea，PD）是一种常见的经期腹痛疾病，通常无盆腔病理变化，但严重影响女性的生活质量，超过40%中国女大学生受到痛经困扰。近年来，软饮料（如碳酸饮料、果汁饮料、乳饮料等）和咖啡的消费在中国显著增加，软饮料含糖量高，咖啡则含有刺激性咖啡因。一些研究表明，高糖软饮料的消费可能与PD发生率增加有关，但咖啡与PD的关系仍存在争议。近日，滨州医科大学研究团队在Scientific Reports发表了题为“Associations between soft drinks intake and primary dysmenorrhea among Chinese undergraduate female students”的研究性论文，首次对中国女大学生的软饮料消费与原发性痛经的发生率之间的关系进行了分析。在这项研究中，共招募了1809名参与者，多数为汉族，平均年龄为19.7岁。47.1%的参与者在过去三个月经历过PD。在PD组中，51.2%曾饮用碳酸饮料，44.8%曾饮用咖啡。PD严重程度分布如下：轻度24.4%、中度49.6%、重度26.0%。非PD组和PD组在月经周期上相似，PD组中62.3%的参与者月经量较大，非PD组中37.7%的参与者月经量较大。研究发现，碳酸软饮料的摄入与PD发生率的增加相关，碳酸饮料组的PD发生率比非碳酸饮料组高1.24倍。此外，碳酸饮料的摄入频率与PD的严重程度呈剂量反应关系：摄入频率越高，痛经的严重程度越重。这与高糖饮料引发的营养不平衡所导致肌肉痉挛、以及排卵周期子宫内膜中前列腺素（PGs）水平升高有关，尤其是前列腺素F2α在痛经女性中的浓度更高，导致子宫收缩和疼痛感受器激活。此外，高糖饮料还可能通过提高皮质醇水平影响PD的发展，皮质醇与压力反应系统失调相关。相比之下，咖啡的摄入则可能降低痛经风险。研究显示，饮用咖啡的中度至重度痛经参与者出现严重症状的几率较低，但咖啡饮用频率与痛经严重程度之间未发现显著关联。研究人员推测，咖啡因可能通过收缩血管和减少子宫血流量来减轻痛经，目前也有研究表明咖啡因可能通过增加前列腺素E1（PGE1）来放松子宫。总体而言，本研究表明，频繁摄入碳酸软饮料会增加女性痛经的风险，而适量饮用咖啡可能对缓解痛经有所帮助。因此，预防痛经或需要重视限制碳酸饮料的摄入，并根据自身情况，适量饮用咖啡。参考文献： Wang, L., Wen, S., Li, X. et al. Associations between soft drinks intake and primary dysmenorrhea among Chinese undergraduate female students. Sci Rep 14, 21210 (2024). https://doi.org/10.1038/s41598-024-71802-8 本文仅用于学术分享，转载请注明出处。若有侵权，请联系微信：bioonSir 删除或修改！点击下方「阅读原文」，前往生物谷官网查询更多生物相关资讯~

临床研究

2024-09-10

·生物探索

Cell Metab丨肖玲等揭示棕色脂肪中的m⁶A mRNA甲基化通过独立于UCP1的前列腺素信号调控全身胰岛素敏感性

引言棕色脂肪组织（BAT）因其通过解偶联蛋白1（UCP1）产生热量，成为治疗和预防肥胖及相关代谢疾病的潜在靶点。研究表明，冷刺激激活的BAT可以通过能量消耗减少体脂，改善心脏代谢健康，特别在超重和肥胖个体中效果显著。尽管BAT的产热功能备受关注，近年来越来越多的证据表明，BAT还通过分泌各种有益因子（"BATokine"，如肽类、蛋白质、脂质、miRNA等）对全身代谢产生积极调节。其中，一类被称为“BAT lipokine”的脂质因子尤为重要【1,2】，它与胰岛素敏感性、葡萄糖耐量以及炎症调节密切相关。 m6A（N6-甲基腺嘌呤）是RNA中最常见的内部甲基化修饰之一，广泛存在于真核生物的mRNA和非编码RNA中，在RNA剪接、翻译、稳定性和降解等过程中发挥重要作用。m6A修饰与干细胞分化、肿瘤发生、免疫调控和代谢紊乱等生理与病理过程密切相关。尽管已知m6A在棕色脂肪产热中具有重要作用，但m6A是否通过调控棕色脂肪分泌的"BATokine"影响全身代谢尚不清楚。 2024年9月9日，哈佛医学院及Joslin糖尿病研究中心（Joslin Diabetes Center）的Rohit N. Kulkarni教授团队在Cell Metabolism在线发表了题为m6A mRNA Methylation in Brown Fat Regulates Systemic Insulin Sensitivity via an Inter-Organ Prostaglandin Signaling Axis Independent of UCP1的研究成果。该研究通过基因组学、甲基化组学和脂质组学等多组学手段，首次揭示了：1. 由甲基转移酶14（METTL14）介导的m6A修饰，通过负向调控BAT中前列腺素合成酶的表达，抑制了前列腺素PGE2和PGF2α的合成；2. 敲除BAT中的METTL14显著提高了PGE2和PGF2α的水平，从而改善了胰岛素敏感性，促进了白色脂肪棕色化，并降低了肥胖小鼠体重；3. 揭示了PGE2和PGF2α作为新型胰岛素增敏剂的新分子机制。研究者首先检测了不同胰岛素抵抗小鼠模型中BAT的m6A甲基转移酶水平，发现胰岛素抵抗小鼠BAT中METTL14高表达，提示其与胰岛素敏感性负相关。进一步通过特异性敲除小鼠BAT中的METTL14，结果显示，敲除后小鼠全身多种代谢组织的胰岛素敏感性显著提升。这表明METTL14的缺失能够通过BAT与其他代谢组织的相互作用改善胰岛素敏感性，且不依赖于UCP1的产热功能。通过脂质组学分析，研究者发现，在METTL14缺失的小鼠棕色脂肪组织及人源性棕色脂肪细胞中，PGE2和PGF2α的水平显著升高。进一步研究表明，PGE2和PGF2α能够显著增强代谢细胞的胰岛素敏感性。在肥胖小鼠模型中注射这两种前列腺素，表现出胰岛素增敏及抗肥胖的表型特征，并伴随白色脂肪组织的米色化现象。值得注意的是，PGE2和PGF2α的血液水平在肥胖及胰岛素抵抗人体中与胰岛素敏感性呈显著正相关。机制研究进一步揭示，PGE2和PGF2α通过特异性GPCR介导的信号通路，抑制AKT磷酸酶的活性，从而提高胰岛素敏感性。模式图（Credit: Cell Metabolism）为了进一步研究m6A如何调控BAT中前列腺素的合成，研究者结合基因组学和m6A甲基化组学，发现METTL14的缺失通过降低m6A甲基化水平，增强了前列腺素合成酶基因的稳定性，增加了其表达。综上所述，该研究揭示了m6A通过调控前列腺素在棕色脂肪中的合成，进而影响全身代谢的新机制，拓展了m6A及其他表观遗传修饰在代谢调控中的研究思路。模式图（Credit: Cell Metabolism）参考文献 1 SHAMSI, F.; WANG, C. H.; TSENG, Y. H. The evolving view of thermogenic adipocytes - ontogeny, niche and function. Nat Rev Endocrinol, v. 17, n. 12, p. 726-744, Dec 2021. ISSN 1759-5029 (Print) 1759-5029. 2 CYPESS, A. M. Reassessing Human Adipose Tissue. N Engl J Med, v. 386, n. 8, p. 768-779, Feb 24 2022. ISSN 0028-4793. 3 DE JESUS, D. F. et al. m(6)A mRNA Methylation Regulates Human β-Cell Biology in Physiological States and in Type 2 Diabetes. In: (Ed.). Nat Metab. Germany, v.1, 2019. p.765-774. ISBN 2522-5812 (Electronic) 2522-5812 (Linking). 4 D, F. D. J. et al. Redox regulation of m(6)A methyltransferase METTL3 in β-cells controls the innate immune response in type 1 diabetes. Nat Cell Biol, v. 26, n. 3, p. 421-437, Mar 2024. ISSN 1465-7392. 5 KAHRAMAN, S. et al. m6A mRNA Methylation Regulates Early Pancreatic β-Cell Differentiation. bioRxiv, p. 2023.08.03.551675, 2023. Disponível em: < https://www.biorxiv.org/content/biorxiv/early/2023/08/03/2023.08.03.551675.full.pdf >. https://doi.org/10.1016/j.cmet.2024.08.006 责编|探索君排版|探索君文章来源|“BioArt” End 往期精选围观一文读透细胞死亡(Cell Death) | 24年Cell重磅综述（长文收藏版）热文 Cell | 是什么决定了细胞的大小？热文 Nature | 2024年值得关注的七项技术热文 Nature | 自身免疫性疾病能被治愈吗？科学家们终于看到了希望热文 CRISPR技术进化史 | 24年Cell综述

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