Phase II Trial O6-benzylguanine(BG) and Temozolomide(TMZ) Therapy of Glioblastoma Multiforme (GBM) With Infusion of Autologous P140K MGMT+Hematopoietic Progenitors to Protect Hematopoiesis

This phase II trial studies the effect of P140K MGMT hematopoietic stem cells, O6-benzylguanine, temozolomide, and carmustine in treating participants with supratentorial glioblastoma or gliosarcoma who have recently had surgery to remove most or all of the brain tumor (resected). Chemotherapy drugs, such as 6-benzylguanine, temozolomide, and carmustine, work in different ways to stop the growth of tumor cells, either by killing the cells, by stopping them from dividing. Placing P140K MGMT, a gene that has been created in the laboratory into bone marrow making the bone more resistant to chemotherapy, allowing intra-patient dose escalation which kills more tumor cells while allowing bone marrow to survive.

开始日期2023-01-20

申办/合作机构

The Case Comprehensive Cancer Center

[+1]

NCT02343666

/ Withdrawn临床1期IIT

A Clinical Trial of Gene-Modified Stem Cells to Generate HIV-Resistant Cells in Conjunction With Standard Chemotherapy for Treatment of Lymphoma in Patients With HIV Infection

This pilot phase I trial studies the side effects and best dose of human immunodeficiency virus (HIV)-resistant gene modified stem cells in treating HIV-positive patients who are undergoing first-line treatment for Hodgkin or Non-Hodgkin Lymphoma. Stem cells are collected from the patient and HIV-resistance genes are placed into the stem cells. The stem cells are then re-infused into the patient. These genetically modified stem cells may help the body make cells that are resistant to HIV infection.

开始日期2016-08-15

申办/合作机构

Fred Hutchinson Cancer Research Center

[+2]

NCT01769911

/ WithdrawnN/AIIT

AUTOLOGOUS TRANSPLANTATION AND STEM CELL BASED-GENE THERAPY FOR THE TREATMENT OF HIV-ASSOCIATED LYMPHOMA

This clinical trial studies genetically modified peripheral blood stem cell transplant in treating patients with HIV-associated non-Hodgkin or Hodgkin lymphoma. Giving chemotherapy before a peripheral stem cell transplant stops the growth of cancer cells by stopping them from dividing or killing them. After treatment, stem cells are collected from the patient's blood and stored. More chemotherapy or radiation therapy is then given to prepare the bone marrow for the stem cell transplant. Laboratory-treated stem cells are then returned to the patient to replace the blood-forming cells that were destroyed by the chemotherapy and radiation therapy

开始日期2015-02-01

申办/合作机构

Fred Hutchinson Cancer Research Center

[+1]

100 项与苄基鸟嘌呤相关的临床结果

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100 项与苄基鸟嘌呤相关的转化医学

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100 项与苄基鸟嘌呤相关的专利（医药）

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739

项与苄基鸟嘌呤相关的文献（医药）

2025-06-01·Molecular Therapy-Methods & Clinical Development

In vivo HSC transduction in humanized mice mediated by novel capsid-modified HDAd vectors

Article

作者: Li, Chang ; Wang, Hongjie ; Georgakopoulou, Aphrodite ; Lieber, André ; Kim, Jiho

We developed an in vivo hematopoietic stem cell (HSC) gene therapy approach consisting of HSC mobilization and intravenous injection of helper dependent adenovirus (HDAd) vectors. While we have demonstrated safety and efficacy of the in vivo approach in CD46-transgenic mice and rhesus macaques, studies in mice with a humanized hematopoietic system could facilitate its potential clinical translation for the treatment of hemoglobinopathies and HIV. Using mild myelo-conditioning in NSGW41 mice and cryopreserved human CD34⁺ cells from healthy donors we achieved ∼10% human chimerism in peripheral blood. Engrafted primitive human CD45⁺/CD34⁺/CD90⁺-HSCs efficiently mobilized by different approaches involving AMD3100 in combination with granulocyte colony-stimulating factor G-CSF, truncated Groβ (tGroβ), or WU106/tGroβ. At the peak of mobilization, integrating HDAd-GFP vectors were injected intravenously followed by O⁶BG/BCNU in vivo selection. Long-term stable GFP expression was shown for HDAd5/35 and the new vector platforms HDAd6/3 and HDAd5/35_lam, a fiber/penton-modified vector. Two months post transduction, GFP marking in the periphery were 22.38% (8.17%), 41.12% (10.62%), and 32.15% (4.49%) for HDAd5/35, HDAd6/3, and HDAd5/35_lam, respectively. GFP levels in bone marrow were 33.53% (8.96%), 53.51% (6.95%), and 33.29% (5.21%) and in spleen 32.6% (9.25%), 33.75% (5.47%), and 20.79% (6.15%). Our study describes a new animal model for in vivo HSC transduction with HDAds, with implications for studies with other vectors.

2025-05-01·AMERICAN JOURNAL OF HUMAN GENETICS

Identifying active and inhibitor-resistant MGMT variants for gene therapy

Article

作者: Fisher, Adam ; Bashyam, Ashvin ; Forget, Anthony ; Nagel, Zachary David ; Peters, Robert ; Cheong, Ana

O6-methylguanine-DNA methyltransferase (MGMT) reverses alkylating-agent-induced methylation by 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) at the O⁶ position of guanine. MGMT is irreversibly inhibited by O⁶-benzylguanine (O6BG), while the Pro140Lys (P140K) variant is resistant. Combining the use of O6BG/BCNU with gene transfer of MGMT P140K into hematopoietic stem cells (HSCs) has enabled in vivo enrichment of gene-modified HSCs for therapeutic effect in preclinical studies. However, the P140K substitution cannot reliably be made using currently available gene-editing approaches. Identifying functional MGMT variants that are resistant to inhibitors and amenable to gene editing would enable in vivo enrichment of HSCs edited at both MGMT and a therapeutic locus. We used computational analyses to select putative variants and generated a library of MGMT variant-expressing plasmids (pMGMTs). For our functional screen, we treated MGMT-deficient U251 cells with O6BG and co-transfected them with pMGMT together with a plasmid cocktail including a fluorescent host cell reactivation reporter plasmid (mPlum_O⁶MeG) for MGMT activity. Flow cytometric analysis of MGMT activity identified active and O6BG-resistant MGMT variants. Treatment with a second MGMT inhibitor, PaTrin-2, confirmed these results. We also found MGMT variants that are detectable in the general population and tumors to be active and O6BG sensitive. Taken together, our findings establish a functional database for MGMT variants and a cell-based platform for screening DNA-repair proteins for unknown functional properties.

2025-03-12·ACS Applied Materials & Interfaces

Enzyme Reactions Are Accelerated or Decelerated When the Enzymes Are Located Near the DNA Nanostructure

Article

作者: Nakata, Eiji ; Morii, Takashi ; Lin, Peng ; Kinoshita, Masahiro ; Hayashi, Tomohiko ; Dinh, Huyen

It is known experimentally that enzymatic reactions are often accelerated when the enzymes are assembled on the scaffold of DNA nanostructures. However, the exact mechanism by which this acceleration occurs remains unclear. Here, we study the reactions of enzymes with different catalytic mechanisms assembled on a DNA scaffold with various substrates. Analysis of the hydration properties of the substrates using our accurate statistical mechanics theory classifies the substrates into two groups that behave as hydrophilic and hydrophobic solutes, respectively. The reaction of the enzyme on the DNA scaffold is accelerated with a hydrophilic substrate but decelerated with a hydrophobic substrate. We propose a mechanism of acceleration or deceleration in which, due to the formation of a high-density layer of water near the DNA surface with high negative charge density, the concentration of a substrate with high energetic affinity for water within the layer becomes higher than that near a free enzyme, whereas that of a substrate with low energetic affinity becomes lower within the layer. This study provides chemical and physical insights into a general case of biocatalysts, where the rates of chemical reactions occurring at the interface of biomolecules in aqueous environments can differ substantially from those in the bulk solution due to variations in the local concentration of a given ligand.

项与苄基鸟嘌呤相关的新闻（医药）

2024-12-11

·CPHI制药在线

酵母微胶囊技术的研究应用进展

关注并星标CPHI制药在线微囊化是一种固定化技术，其通过提供一个物理屏障克服外界环境对包封分子的影响，从而提高包合物的稳定性。酵母是一类单细胞真菌，酵母及其生物制剂已经广泛应用在食品工业、生物医药等领域。酵母微囊从酵母中提取，其成分主要是 β-葡聚糖，形状呈椭圆形，具有约2-5 μm 的中空多孔结构。β-葡聚糖是酵母细胞壁的主要结构成分，在结构上主要由 β-1,3-葡聚糖的主链和β-1,6-葡聚糖的侧链组成，其单糖组成为葡萄糖。其中 β-1,3-葡聚糖既构成了葡萄糖刚性网络结构，也为酵母微囊提供了免疫活性。β-1,3-葡聚糖是真菌的主要病原体相关分子模式，它能够被肠道免疫细胞通过多种模式识别受体(包括补体受体 3 和 Dectin-1 受体)识别出来，从而引起相关免疫反应。研究对比多种微生物（如霉菌、原生虫、酵母菌）作为壁材的包埋效果，结果发现采用酵母微胶囊包埋效果最理想。对于所有的包封体系，包封分子与基质之间的相互作用对其稳定性和生物利用度都有着重要的影响。而经酵母包埋后的活性物质稳定性、生物利用度、溶解性和缓释性均有所提高。酵母细胞以分散的单细胞状态存在，其完整的细胞壁和细胞膜结构具有一定的强度和通透性，从而使酵母细胞具有很好的吸附性能，可作为一种新型的微胶囊壁材。酵母细胞有一层磷脂膜，因此它可以充当脂质体，既可以封装疏水化合物，也可以封装亲水化合物。而且酵母微囊由β-葡聚糖构成了一个内部中空、表面多孔且带负电荷的结构，因此大多数的药物都可以在酵母微囊的内部被封装或者吸附在酵母微囊表面，有利于药物的递送。随着研究的不断发展，酵母微囊的药物装载策略逐渐增多，主要包括以下5 种。 (1) 通过静电吸附作用将带正电的药物装载到酵母微囊内部。例如，有学者制备了带有正电荷的顺铂(cisplatin, CDDP)前体纳米粒(PreCDDP)，并将其封装到酵母微囊中，开发了一种安全有效的口服顺铂药物递送平台。此外，通过在酵母微囊表面负载带有相反电荷的胰岛素，并用海藻酸盐进行包被，可成功实现胰岛素的口服递送。 (2) 对于带负电荷的药物，可以通过非共价作用力修饰带正电荷的载体材料，再通过静电吸附作用装载到酵母微囊中。如首先将小檗碱(berberine, BBR)、表没食子儿茶素没食子酸酯(epigallocatechin gallate, EGCG)和 Mn2+通过非共价分子间作用力自组装成纳米颗粒(BBR/MPNNPs)，而后进一步装载到酵母微囊中。将DOX装载在3-巯基丙酸(3-mercaptopropionic acid, MPA)修饰的纳米氧化锌(ZnO NPs)上，构建了带负电荷的载药纳米粒(MPA-ZnO-DOX)，再用聚乙烯亚胺(polyethyleneimine, PEI)将酵母微囊内部修饰成正电荷，使 MPA-ZnO-DOX 能够装载在酵母微囊中。 (3) 可以利用被动扩散或疏水作用将不带电的药物修饰进酵母微囊中。如通过被动扩散将牛血清蛋白(bovine serum albumin, BSA)包封在被壳聚糖包被的酵母微囊里。此外，咖啡因、维生素 B12 等都可以通过相互孵育利用被动扩散装载到酵母微囊中。 (4) 部分药物还可以通过逐层合成(LBL)进行装载。通过静电相互作用将带有相反电荷的多糖依次沉积在预先装载花青素的酵母微囊上，制备出用于花青素包封和稳定的口服递送载体。如将替莫唑胺 (temozolomide,TMZ) 装载在聚乳酸-羟基乙酸共聚物 (poly(lactic-co-glycolic acid), PLGA)中，并通过逐层合成依次包裹 O6-苄基鸟嘌呤(O6-benzylguanine,O6-BG)接枝的壳聚糖(BG-chitosan, BG-CS)层和酵母微囊，最终获得了一个稳定的 TMZ 口服递送平台。 (5) 某些药物还可以将其与衍生化的酵母微囊进行结合。如使用原位合成酵母微囊包覆镍纳米颗粒，将带有His-tag的Cda2 蛋白通过非共价作用力装载进酵母微囊中。使用 NaIO4 对酵母微囊进行氧化，使其具有醛基，进而与卵清蛋白(ovalbumin, OVA)的伯胺基反应，将 OVA 通过化学连接到酵母微囊表面，从而在体内产生有效的特异性免疫反应。酵母微胶囊技术作为口服药物递送系统的研究应用 1、降血糖 Ⅰ型糖尿病是一种与碳水化合物代谢受损相关的代谢紊乱，可导致慢性高血糖，该疾病的特征是β 细胞进行性自身免疫破坏，导致胰岛素不足或胰岛素分泌缺陷。胰岛素皮下注射用于糖尿病的日常治疗。但是由于胰岛素给药带来的不便，很难满足患者的依从性。为了克服这个困难，可优先选择口服胰岛素输送系统。然而，口服胰岛素必须克服某些生理障碍。侵入性和强化方法促使人们需要一种相容的胰岛素给药途径。酿酒酵母的微胶囊可以通过静电力介导的自发沉积结合不同的带电纳米粒子，长期服用酵母微胶囊在治疗慢性疾病方面显示出良好的安全性。有研究首次提出了一种利酵母微胶囊包埋胰岛素抑制糖尿病的生物激励方法。利用胰岛素和酵母微胶囊之间的静电相互作用促使酵母微胶囊包埋胰岛素的形成，并在荧光成像下证实了这一点。在酵母微胶囊包埋胰岛素的表面装载海藻酸钠涂层可以保护酵母微胶囊免受恶劣的胃肠道环境影响，防止胰岛素降解。药理学实验发现，糖尿病大鼠口服海藻酸钠包覆的酵母微胶囊包埋胰岛素后血糖明显降低。 2、治疗心血管疾病心血管疾病的发生和动脉硬化紧密相关。近年来，基于纳米粒子的靶向策略被认为是治疗动脉粥样硬化（AS）的新途径。有学者研发了一种用于靶向治疗心血管疾病的方法，该方法由酵母衍生微胶囊（YC）介导，利用静电驱动自发沉积的方式使不同带电纳米颗粒成功负载到 YC 中。口服给药后，包埋纳米颗粒的酵母胶囊（YDMC）可以优先到达关节炎和肿瘤的病变部位，并显著降低斑块面积，增强 AS 斑块的稳定性。最重要的是，实验发现 YC介导的纳米疗法比未包装的纳米药物更有效，对毒性测定的结果显示包装后的纳米药物在长期口服后表现出良好的安全性，因此该方法或可用于 AS 和其他血管疾病的靶向治疗。单核细胞趋化蛋白 -1（MCP-1）在 AS 中扮演着关键的角色，是 AS 发生早期的重要因素。有学者利用上述YC 介导的仿生方法成功将一种 MCP-1 的特异性合成抑制剂靶向输送到 AS 斑块内部，结果显示这种抑制剂的作用明显增强，有效减少了单核细胞在 AS 斑块中的聚集。 3、治疗炎症相关疾病胃肠道存在许多生物屏障，即使是当前最先进的纳米粒子靶向传递系统口服给药后也面临来自胃肠道障碍的挑战。有学者尝试将含有消炎药吲哚美辛或抗肿瘤药物紫杉醇的纳米粒负载到酵母胶囊中，口服后经过一系列的体内运输过程，包埋在酵母胶囊中的纳米粒最终被传递到小鼠或大鼠炎症或肿瘤的远程病变部位。结果显示，在该急性炎症模型中，酵母胶囊介导的纳米探针的靶向效率甚至比静脉注射相同剂量的纳米探针的靶向效率还要高。口服经酵母胶囊介导的吲哚美辛或紫杉醇纳米疗法在炎症和肿瘤动物模型中与单独纳米治疗相比，作用显著增强。利用酵母细胞包封姜黄素研究其在体外的抗炎作用，发现克服姜黄素生物利用度低、提高靶向传递主要是酵母细胞中纯化的葡聚糖颗粒发挥作用。 4、抗肿瘤口服是最常见和最有效的给药方式，具有较高的安全性、方便性和用药依从性。目前，口服抗肿瘤药物在治疗癌症方面越来越受欢迎。但一些治疗癌症的口服药物存在亲水性低、对上皮细胞渗透性差、毒副作用大等问题。卡巴他赛是一种新型半合成紫杉醇，具有很强的抗肿瘤活性，与其他紫杉醇类似，卡巴他赛的口服给药仍然受到限制，有学者将载药聚合物-脂质杂化纳米粒子（NPs）负载到多孔空心酵母细胞壁微颗粒（YPs）中，制备了一种载药纳米微载体，用于巨噬细胞靶向口服卡巴他赛；在模拟胃肠道环境中，NPs 负载 YPs的体外释药速度较 NPs 慢，药物稳定性较高；体内药动学研究中，卡巴他赛溶液的口服利用度提高了5倍。顺铂是当前联合化疗中最常用的药物之一，将制备好的顺铂衍生的前体纳米粒子（PreCDDP）封装到酵母胶囊中得到可以口服给药的 PreCDDP/YC，并从其理化性质、体外药物释放、体外抗肿瘤活性、口服靶向性、体内药动学和体内药效等方面对酵母胶囊仿生给药系统进行考察，结果 PreCDDP/YC在细胞内释放后仍具有抗肿瘤活性，口服载于酵母胶囊中的 PreCDDP 比口服或静脉注射游离的顺铂具有更好的安全性和更高的生物利用度。上述研究表明载药纳米微载体技术是增加小分子药物口服吸收的一种可能途径。酵母微囊作为口服递送载体具有诸多优势。首先，因为人体胃肠道中缺乏可以降解 β-葡聚糖的酶，而且 β-葡聚糖具有耐酸的特性，所以以 β-葡聚糖为主要成分的酵母微囊可以避免在恶劣的肠道环境中被消化降解，保护药物可以顺利通过复杂的肠道环境而不被破坏。其次， β-葡聚糖是一种来源于酵母细胞的载体，因此可以模仿酵母的行为，被 M 细胞上的 Dectin-1 识别，从而通过复杂的巨噬细胞介导运动，将各种药物准确、安全地靶向地送到病变部位。酵母微囊对极性和非极性的小分子具有良好的渗透性，而且可以通过调节表面紧密连接程度促进对生物大分子的吸收，所以可以有效地装载各类大分子和小分子的药物，例如蛋白质、DNA、抗原、姜黄素等。最后，酵母微囊具有无细胞毒性以及安全的特点，这是作为口服递送载体的一大根本要求。参考资料 [1]李文,刘夫锋.基于益生菌口服药物递送系统的研究进展[J].微生物学报,2024,64(11):4086-4105. [2]贾丽娟,戴智强,高青青,等.酵母微胶囊包埋新技术及药效评价的研究进展[J].中南药学,2022,20(04):871-876. 作者简介：小泥沙，食品科技工作者，食品科学硕士，现就职于国内某大型药物研发公司，从事营养食品的开发与研究。 END 【智药研习社近期直播预告】来源：CPHI制药在线声明：本文仅代表作者观点，并不代表制药在线立场。本网站内容仅出于传递更多信息之目的。如需转载，请务必注明文章来源和作者。投稿邮箱：Kelly.Xiao@imsinoexpo.com ▼更多制药资讯，请关注CPHI制药在线▼ 点击阅读原文，进入智药研习社~

2023-05-09

·FierceBiotech

SNAPing together a universal receptor system for T cell therapies

The researchers designed two different types of receptors containing SNAPtag: chimeric antigen receptors and SynNotch receptors, synthetic receptors that can be programmed to turn on or off virtually any gene T cell therapies work well for blood cancers, but they come with caveats—a major one being that they’re cumbersome and expensive to tailor to a patient’s specific cancer type. On top of that, the therapy must be re-customized to home in on a new target each time the cancer evolves. Now, a new universal receptor system designed by a research team from the University of Pittsburgh could make engineering custom T cell therapies far more efficient. Part of the company’s technology has already been licensed by Pennsylvania-based biotech Coeptis Therapeutics. In a study published May 9 in Nature Communications, the scientists described how they engineered T cells with receptors containing a special enzyme called SNAPtag. The engineered cells are administered alongside antibodies for proteins specific to a patient’s cancer. These antibodies are labeled with a molecule called benzylguanine, which forms a covalent bond—the strongest type there is—with SNAPtag. This welds the antibodies to the receptor so it’s able to recognize the cancer cells. “We wanted to make receptors that wouldn’t just bind with the adapter antibodies—it would actually fuse with them,” senior author Jason Lohmueller, Ph.D., told Fierce Biotech Research. “This would allow them to have more potent activity so we could get by with a lower amount of that antibody and get more sensitive responses.” Lohmueller’s lab, together with the lab of Alexander Deiters, Ph.D., also at the University of Pittsburgh, is one of the first to use SNAPtag in a therapeutic setting. Benzylguanine by itself is an antineoplastic agent that’s been used to make tumor cells more vulnerable to cancer drugs. SNAPtag and benzylguanine together have been used in imaging in animals and to label proteins. “Before, the enzyme was being viewed more as a tool,” Lohmueller explained. The researchers designed two different types of receptors containing SNAPtag: chimeric antigen receptors and SynNotch receptors, synthetic receptors that can be programmed to turn virtually any gene on or off. Importantly, multiple antibodies can be fused to the receptor at once or in succession. That makes it theoretically possible to head off mutations, prevent relapse and circumvent the costly process of re-engineering T cells for another round of therapy, Lohmueller said. The researchers tested out their system in human cells and mouse models, targeting human CD20 and HER2 to assess its potency against blood cancer and solid tumor antigens. All of the mice that received SNAP-CAR T cells along with the antibodies showed significant tumor regressions. Around 80% of the mice appeared to be tumor-free in the experiments targeting the HER2+ tumors. There were five mice per group and the experiments were replicated multiple times, according to Lohmueller. The team is now working on gathering data about the technology’s efficacy against other tumor types. Coeptis has licensed the company’s SNAP-CAR system and plans to first target HER2-expressing cancer in a potential clinical program, according to Vice President of Operations Dan Yerace. Then, the biotech will identify additional targets with the hopes of addressing unmet medical needs in oncology.

细胞疗法

100 项与苄基鸟嘌呤相关的药物交易

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外链

KEGG	Wiki	ATC	Drug Bank
-	苄基鸟嘌呤	-	DB11919

研发状态

10 条进展最快的记录,

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适应症	最高研发状态	国家/地区	公司	日期
多形性胶质母细胞瘤	临床3期	美国	-	2001-09-01
神经胶质肉瘤	临床3期	美国	-	2001-09-01
皮肤难治性T细胞淋巴瘤	临床2期	美国	National Cancer Institute	2010-02-01
塞扎里综合征	临床2期	美国	National Cancer Institute	2010-02-01
混合型少突星形细胞瘤	临床2期	美国	Keryx Biopharmaceuticals, Inc.	2006-10-01
少突神经胶质瘤	临床2期	美国	Keryx Biopharmaceuticals, Inc.	2006-10-01
脑干肿瘤	临床2期	美国	National Cancer Institute	2006-02-01
高级别胶质瘤	临床2期	美国	National Cancer Institute	2006-02-01
复发性胶质母细胞瘤	临床2期	美国	Duke University	2004-04-01
局部晚期软组织肉瘤	临床2期	美国	The University of Chicago	2000-06-01

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临床结果

适应症

分期

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研究	分期	人群特征	评价人数	分组	结果	评价	发布日期


NCT01269424 (ASCO2019)	临床1期	胶质母细胞瘤	10	O6BG (MGMT-P140K transfected hematopoetic progenitor cells)	淵襯願窪醖壓築獵鹽夢(範鑰壓壓製鬱鹹顧醖糧) = 3/10 patients suffering grade 3-4 hematologic toxicity 鑰餘齋襯獵廠繭鹹艱壓 (鏇鹽顧襯簾鑰廠憲鹽鹽 ) 更多	积极	2019-05-26
NCT00669669 (CSCGT, CTgov)	临床1/2期	神经胶质肉瘤 \| 多形性胶质母细胞瘤 \| 胶质母细胞瘤	12	Intensity-Modulated Radiation Therapy+Temozolomide+Carmustine+O6-Benzylguanine+Filgrastim+Plerixafor	艱鑰鑰餘夢餘廠網夢鬱 = 獵範壓廠襯艱壓鬱憲憲觸鏇膚願襯淵艱窪獵遞 (壓壓蓋憲積窪顧餘選鏇, 餘艱構膚繭鑰觸繭繭繭 ~ 網觸選衊鏇窪鏇窪鏇衊) 更多	-	2018-12-28
NCT00961220 (Pubmed \| JAMA Dermatol) 人工标引	临床1/2期	蕈样真菌病	17	Carmustine+O6-Benzylguanine	廠淵鏇壓廠網網鏇遞衊(選壓鹽獵蓋製鹽壓鹽醖) = 襯鬱憲範顧網遞獵齋鏇築顧鏇憲願憲築鹽製鏇 (製繭鏇積餘鏇範糧願憲 ) 更多	积极	2017-05-01
NCT00436436 (CTgov)	临床2期	多形性胶质母细胞瘤	12	temozolomide+O6-benzylguanine	襯壓淵醖選網積鹹糧窪 = 製願鹽鏇鹽鏇窪夢襯鹽艱網獵顧積鹹觸築醖糧 (選鹽構築顧艱壓獵壓構, 鏇糧簾壓觸鬱淵鬱廠鬱 ~ 鏇壓餘選鏇範鹽簾顧鏇) 更多	-	2017-03-28
NCT00017147 (S0001, Pubmed) 人工标引	临床3期	神经胶质肉瘤 \| 胶质母细胞瘤一线	179	BCNU+radiation therapy+O?-benzylguanine	觸構鬱醖壓觸衊獵範獵(艱願襯網醖選蓋夢膚鑰) = 夢觸簾憲鑰鏇選鹽齋醖鬱製衊範鏇積獵顧餘鏇 (鹹願糧膚廠選鬱壓鏇選, 8 ~ 13) 更多	不佳	2015-08-01
NCT00017147 (S0001, Pubmed) 人工标引	临床3期	神经胶质肉瘤 \| 胶质母细胞瘤一线	179	BCNU+radiation therapy	觸構鬱醖壓觸衊獵範獵(艱願襯網醖選蓋夢膚鑰) = 願衊製築簾艱獵選構糧鬱製衊範鏇積獵顧餘鏇 (鹹願糧膚廠選鬱壓鏇選, 8 ~ 12) 更多	不佳	2015-08-01
NCT00961220 (CTgov)	临床1/2期	塞扎里综合征 \| 皮肤难治性T细胞淋巴瘤	17	Laboratory Biomarker Analysis+Carmustine+O6-Benzylguanine	鏇憲觸壓顧選窪顧廠夢 = 齋積積憲鹽膚夢鹽襯襯膚獵鑰願鏇鏇鹹襯範餘 (簾鏇顧艱選鏇遞鬱鹹廠, 醖鹽顧顧襯壓醖醖鏇膚 ~ 鏇顧製蓋膚夢鏇遞淵鹽) 更多	-	2015-03-23
AACR2014	N/A	乳腺癌	-	Fulvestrant	憲襯窪網築憲願齋襯廠(獵淵積鹹夢鬱鑰餘蓋夢) = 膚鏇築襯鑰願繭廠簾鏇餘憲遞膚蓋鑰鑰鑰鑰顧 (窪憲遞衊蓋觸顧艱鑰顧 )	-	2014-10-01
NCT00275002 (CTgov)	临床2期	脑干肿瘤 \| 高级别胶质瘤	41	Temozolomide+O6-Benzylguanine (Recurrent High-Grade Gliomas)	積憲窪膚鏇願鹹網襯醖 = 鹽觸觸築襯襯蓋糧顧築蓋窪觸鏇願齋餘願顧繭 (憲鏇憲選膚蓋選築鏇網, 憲簾遞餘糧鑰範膚鏇選 ~ 鬱鹽鏇鹽構衊餘夢構積) 更多	-	2011-08-09
NCT00275002 (CTgov)	临床2期	脑干肿瘤 \| 高级别胶质瘤	41	Temozolomide+O6-Benzylguanine (Recurrent Brain Stem Tumors)	積憲窪膚鏇願鹹網襯醖 = 鑰夢鹽糧蓋積繭襯鏇選蓋窪觸鏇願齋餘願顧繭 (憲鏇憲選膚蓋選築鏇網, 選積獵糧壓艱廠網窪憲 ~ 壓構鹽壓構簾齋膚願窪) 更多	-	2011-08-09
AACR2009	临床2期	脑干肿瘤 \| 复发性恶性胶质瘤	-	O6-Benzylguanine	顧觸範壓鏇選網艱獵餘(醖膚範鏇鏇蓋艱積廠鬱) = 壓鹹願壓繭窪範網遞獵襯艱齋壓窪艱醖艱網構 (鹹艱願構顧襯醖顧築鬱 )	-	2009-05-01
AACR2009	临床2期	脑干肿瘤 \| 复发性恶性胶质瘤	-	Temozolomide	顧觸範壓鏇選網艱獵餘(醖膚範鏇鏇蓋艱積廠鬱) = 膚獵觸夢鬱齋鬱淵夢壓襯艱齋壓窪艱醖艱網構 (鹹艱願構顧襯醖顧築鬱 )	-	2009-05-01
NCT00003765 (Pubmed \| Pediatr Blood Cancer)	临床1期	中枢神经系统肿瘤	36	O6-benzylguanine	積襯艱襯範鬱網齋觸簾(遞醖廠衊衊繭築鹹鏇網) = 繭膚構顧醖選鹽構遞醖願積衊艱繭壓簾窪構顧 (獵鑰襯艱蓋構鏇膚範網 ) 更多	-	2008-03-01