Objective To explore and find out different sealing gas for ampoule sealing to nitrite faild solution of sterile water for injection detection process. Methods In the process of sterile water for injection in the production, each working procedure were collected and tested resp., and would be used for sealing by oxygen - natural gas, oxygen - hydrogen combustion ampoule filling on sterile water for injection, the effect of different sealing gas for sterile water for injection were detected, according to second section of China pharmacopoeia (2015 Edition), nitrite and elec. conductivity in sterile water for injection were detected. Results Before and after oxygen-gas flame and oxygen hydrogen flame two sealing device for ampoule sealing, each sampling position taken by sterile water samples were qualified with conductivity value, which didn′t exceed the limit value of 25 μS/cm; Oxygen-gas sealing device for ampoule sterilization after nitrite melting before sterile water for injection failed the test results, test results of the oxygen-hydrogen gas sealing device for nitrite ampoule after the bottle melting were qualified, and the two test results were consistent. Conclusion The oxygen-hydrogen combustion can be sealed for ampoule, and preparation for the reliable quality of sterile water for injection, and finally reach the finished product test standard.