Performances of four alk.-resistant protein A affinity chromatog. media and two small mol. affinity chromatog. media were compared to provide a reference for selection of purification procedure for monoclonal antibodies (mAbs). The dynamic binding capacities (DBCs) of four protein A affinity chromatog. media MabSelectSuRe, POROS MabCaptureA, Absolute High Cap and TOYOPEARL AF-rProtein A-650F as well as two small mol. affinity chromatog. media Mabsorbent A2P HF and Fabsorbent F1P HF were determined by plotting breakthrough curves of two purified monoclonal antibodies (mAb1 and mAb2) with retention times of 4, 6 and 8 min, resp. The performance of these six affinity media, including recovery rate and removal of foreign matters, were further investigated with clarified cell culture supernatant containing mAb2. At 5% breakthrough point, the DBCs of these media were 35-73 g/L. The recovery rate of mAb2 by Mabsorbent A2P HF was 89%, while those by other five media were not less than 96%. The residual host cell protein (HCP) contents in mAb2 purified by the six media were less than 2 000 ppm, while the residual protein A contents were less than 20 ppm. Taking into consideration of flow rate, DBC, recovery rate and removal of foreign matters, alk.-resistant protein A or small mol. affinity chromatog. media suitable for downstream purification process of therapeutic antibodies may be selected from the six affinity chromatog. media.